Increased permeability of the capillaries of the rat heart to the plasma albumin with asphyxiation and with perfusion

Sutherland, T. M.; Young, Donald A.

doi:10.1113/jphysiol.1966.sp007854

Cited by 29 publications

(22 citation statements)

References 14 publications

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“…Although the observation that albumin permeation of coronary vessels is higher in these experiments than in vivo is consistent with previous reports (Sutherland and Young, 1966;Young, 1968), the increased k 21 does not necessarily imply vascular damage, but may be accounted for by shifts in pressure gradients across vessel walls when blood is replaced with a lowviscosity asanguineous perfusate.…”

Section: Discussionsupporting

confidence: 92%

Hyaluronidase does not prevent deterioration of vascular functional integrity during reperfusion after no-flow ischemia in isolated rabbit hearts.

Tilton

Cole

Larson

et al. 1985

Circulation Research

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SUMMARY. Effects of hyaluronidase on myocardial water content and distribution, and on coronary vascular hemodynamics and endothelial cell transport function were assessed in isolated rabbit hearts during 3.5 hours of reperfusion after 30 minutes of global, no-flow ischemia. In nonischemic control hearts, perfusion pressure, left ventricular end-diastolic pressure, maximum +dP/dt, and intravascular clearance of radiolabeled albumin remained constant during 5 hours of continuous perfusion, while the mean-transit time and vascular into extravascular space clearance of radiolabeled albumin increased 1.5X and 2.5X baseline, respectively. During reperfusion after 30 minutes of no flow, perfusion pressure increased 53% and interstitial fluid volume increased 2-fold, while left ventricular end-diastolic pressure and maximum +dP/dt returned to control levels. The rate of intravascular clearance of radiolabeled albumin decreased 38%, and the mean-transit time and vascular-into-extravascular space clearance of albumin increased ~3x and 5x baseline, respectively. Hyaluronidase blocked the ischemia-reperfusion-induced increases in total water content and in interstitial fluid volume and reduced the increases in perfusion pressure and mean-transit time of radiolabeled albumin by 40% and 45%, respectively, but did not prevent the increase in albumin vascular-into-extravascular space clearance and the decrease in albumin clearance from the coronary vasculature. These findings indicate that hyaluionidase does not prevent ischemia-reperfusion-induced increases in albumin permeation of the coronary vasculature, and suggest that its protective effect on ischemic myocardium is mediated, instead, by reducing interstitial edema and vascular resistance. (Circ Res 56: [839][840][841][842][843][844][845][846][847][848][849][850] 1985)

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Section: Discussionsupporting

confidence: 92%

Hyaluronidase does not prevent deterioration of vascular functional integrity during reperfusion after no-flow ischemia in isolated rabbit hearts.

Tilton

Cole

Larson

et al. 1985

Circulation Research

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“…The markers used were: [U-14C]D-sorbitol mc/m-mole; Radiochemical Centre, Amersham), non-radioactive sorbitol, sucrose, raffinose and inulin (all from British Drug Houses Ltd., Poole, Dorset). Details of the preparation of the conjugate of Evans Blue and albuimin have been given elsewhere (Sutherland & Young, 1966).…”

Section: Methodsmentioning

confidence: 99%

Factors controlling the washout of the interstitial space of the isolated, perfused rat heart

Young¹

1968

The Journal of Physiology

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SUMMARY1. The time course of the washout of the extracellular markers, inulin, raffinose, sucrose and sorbitol, has been determined for the isolated rat heart perfused for 20 min with Krebs bicarbonate medium containing the marker, and washed out with marker-free perfusate.2. The filtration problem for the perfused rat heart was found to be due to omission of the gassing of the concentrated sodium bicarbonate solution with CO2 in the preparation of the perfusate. This probably caused calcium phosphate to be precipitated. The reduced contractility of the heart was reflected in the washout of the extracellular markers.3. The logarithmic plot of the heart content of the marker against time showed two components. The slower was an exponential process, the rate of which was linearly related to the diffusion coefficient for the various markers.4. The faster component was absent in the quiescent heart, and in the beating heart its contribution to the washout of the interstitial space was related to the strength of contraction.5. Similar washout curves were obtained for Evans Blue-albumin conjugate, which readily penetrates the interstitial space of the isolated rat heart. 6. It is suggested that the two components of efflux are a diffusion process limited by perfusate flow, and bulk movements of fluid.

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“…In the case of the heart the comparison is complicated by the fact that the preparations are not alike. This is important since it has been found that heart capillary permeability may 54 CAPILLARY PERMEABILITY be greatly influenced by experimental conditions (Stubbs & Widdas, 1959;Sutherland & Young, 1966;Young, 1968). Furthermore, comparison of results in the contracting heart with investigations in resting skeletal muscle is complicated by the influence of contractility on transcapillary flux of water and solutes (Stubbs & Widdas, 1959;Young, 1968).…”

Section: Introductionmentioning

confidence: 99%

Heart capillary permeability to lipid‐insoluble molecules

Álvarez¹,

Yudilevich²

1969

The Journal of Physiology

122

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2. The method used to study the exchange was the 'indicator diffusion' technique. It consists in a rapid arterial injection of a mixture containing a diffusible and a non-diffusible molecule, followed by a rapid split collection of the venous outflow, up to 30 sec. The fractional extraction, E, of the diffusible substance was obtained by comparing the relative concentrations of both tracers in injected medium and in each venous sample.3. E for [3H]water was the highest (0.90 + 0.03), and it did not vary with flow. All other molecules had values for E that decreased as flow increased.4. Capillary permeability constant, P, was estimated from PS = -F In(1-E), in which S is the surface area of exchange and F is the blood perfusion rate. To test the validity of the equation, E was measured at different blood perfusion rates. It was found that the equation did not apply at relatively low flows for the more diffusible substances. 5. The average values of P estimated for inulin, sucrose, glucose, glycerol and urea were 0x27, 0-8, 1D0, 1-5 and 3-1 x 105 (cm/sec), respectively. The ratio P/D (in which D is the free diffusion in water constant) was the same for all these substances. This can be interpreted as showing that if pores exist in the capillary endothelium, they must be larger than 80-100 A diameter. It is concluded that the pores could actually be the intercellular slits as previously suggested by electron microscope studies. Endothelial cell participation in the exchange appears to be small except for [3H]water.

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Increased permeability of the capillaries of the rat heart to the plasma albumin with asphyxiation and with perfusion

Cited by 29 publications

References 14 publications

Hyaluronidase does not prevent deterioration of vascular functional integrity during reperfusion after no-flow ischemia in isolated rabbit hearts.

Hyaluronidase does not prevent deterioration of vascular functional integrity during reperfusion after no-flow ischemia in isolated rabbit hearts.

Factors controlling the washout of the interstitial space of the isolated, perfused rat heart

Heart capillary permeability to lipid‐insoluble molecules

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