Increased platelet phospholipase activity in diabetic subjects

Takeda, Haruo; Maeda, Hiroshi; Fukushima, Hideo; Nakamura, Nobuhisa; Uzawa, Haruo

doi:10.1016/0049-3848(81)90038-4

Cited by 36 publications

(11 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…8 The increased collagen-induced TxA 2 synthesis in washed platelets from the diabetic group in this report is in support of findings demonstrating increases in collagen-induced aggregation by platelets from diabetic subjects when these platelets were either suspended in plasma 522 or isolated from plasma. 5 The increased thrombin-induced TxA 2 synthesis reported here in platelets from the diabetic group is in support of Takeda et al, 7 who, using platelets prelabeled with 14 Carachidonic acid, found increased thrombin-induced phospholipase activity in platelets from diabetic subjects, and LaGarde et al, 5 who, also using platelets prelabeled with 14 Carachidonic acid, reported increased thrombin-induced synthesis of TxA 2 in platelets from diabetic subjects.…”

Section: Discussionsupporting

confidence: 88%

“…3 Increased platelet aggregation has been reported in human diabetic subjects 45 and experimental animal diabetes. 6 Increased phospholipase activity 7 and increased synthesis of prostaglandin E (PGE) 8 and the highly potent platelet aggregator thromboxane A 2 49 have been reported in platelets from human diabetic subjects. We have reported increased thrombin-induced TxA 2 and PGE 2 biosynthesis in platelets from streptozotocindiabetic rats.…”

mentioning

confidence: 97%

See 1 more Smart Citation

Interrelation of Platelet Vitamin E and Thromboxane Synthesis in Type I Diabetes Mellitus

et al. 1984

View full text Add to dashboard Cite

Platelet vitamin E content and thromboxane A2 (TxA2) synthesis have been investigated in type I diabetic subjects and age- and sex-matched controls. Platelets, but not plasma, from diabetic subjects contained significantly lower vitamin E levels and synthesized significantly greater amounts of TxA2 when challenged with collagen or thrombin than platelets from control subjects. Conversion of exogenously added arachidonic acid to TxA2 was unaltered between platelets from control and diabetic groups. Platelet vitamin E content from control and diabetic groups combined exhibited a significant negative linear correlation with collagen- and thrombin-induced TxA2 production. These data suggest that low platelet vitamin E levels could be a contributing factor to the increased thromboxane synthesis demonstrated by platelets from the above type I diabetic subjects.

show abstract

Section: Discussionsupporting

confidence: 88%

mentioning

confidence: 97%

Interrelation of Platelet Vitamin E and Thromboxane Synthesis in Type I Diabetes Mellitus

et al. 1984

View full text Add to dashboard Cite

show abstract

“…Such an increase indicates an activation of p38 MAP kinase. Given that activated p38 MAP kinase is involved in the phosphorylation and activation of cPLA 2 , the key enzyme in arachidonic acid release, cPLA 2 activation could be possible in platelets from diabetic patients, in agreement with previous observations (28,29). However, and in contrast to cPLA 2, we show that cyclooxygenase, thromboxane synthase, and lipoxygenase enzymes were not activated, which is consistent with an earlier report (28).…”

Section: Discussionsupporting

confidence: 82%

Diabetic Patients Without Vascular Complications Display Enhanced Basal Platelet Activation and Decreased Antioxidant Status

et al. 2004

View full text Add to dashboard Cite

Vascular complications are the leading causes of morbidity and mortality in diabetic patients. The contribution of platelets to thromboembolic complications is well documented, but their involvement in the initiation of the atherosclerotic process is of rising interest. Thus, the aim of the present study was to evaluate basal arachidonic acid metabolism in relation to the redox status of platelets in both type 1 and type 2 diabetic patients, in the absence of vascular complications, as compared with respective control subjects. For the first time, we show that basal thromboxane B 2 , the stable catabolite of thromboxane A 2 , significantly increased in resting platelets from both type 1 and type 2 diabetic patients (58 and 88%, respectively), whereas platelet malondialdehyde level was only higher in platelets from type 2 diabetic subjects (67%). On the other hand, both vitamin E levels and cytosolic glutathione peroxidase activities were significantly lower in platelets from diabetic patients as compared with respective control subjects. We conclude that platelet hyperactivation was detectable in well-controlled diabetic patients without complications. This abnormality was associated with increased oxidative stress and impaired antioxidant defense in particular in type 2 diabetic patients. These alterations contribute to the increased risk for occurrence of vascular diseases in such patients. Diabetes

show abstract

“…Significantly enhanced phospholipid activity of platelets was described in 35 diabetics in comparison to 27 healthy controls. 203 In another study 90 platelet coagulant activity was increased in diabetics with retinopathy.…”

Section: Other Platelet Factorsmentioning

confidence: 97%

Detection of Prethrombotic States in Patients with Atherosclerotic Lesions

Breddin¹

1986

Semin Thromb Hemost

View full text Add to dashboard Cite

Increased platelet phospholipase activity in diabetic subjects

Cited by 36 publications

References 26 publications

Interrelation of Platelet Vitamin E and Thromboxane Synthesis in Type I Diabetes Mellitus

Interrelation of Platelet Vitamin E and Thromboxane Synthesis in Type I Diabetes Mellitus

Diabetic Patients Without Vascular Complications Display Enhanced Basal Platelet Activation and Decreased Antioxidant Status

Detection of Prethrombotic States in Patients with Atherosclerotic Lesions

Contact Info

Product

Resources

About