Increased poly-β-hydroxybutyrate production from carbon dioxide in randomly mutated cells of cyanobacterial strain Synechocystis sp. PCC 6714: Mutant generation and characterization

Kamravamanesh, Donya; Kovács, Tamás; Pflügl, Stefan; Druzhinina, Irina S.; Kröll, Paul; Lackner, Maximilian; Herwig, Christoph

doi:10.1016/j.biortech.2018.06.057

Cited by 95 publications

(56 citation statements)

References 48 publications

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“…carbon source [27,28], which is significantly above the other reported values from literature. The strain had been subjected to UV light mutations to increase the PHB productivity.…”

Section: Phb Production By Cyanobacteria: Current State Of Knowledgementioning

confidence: 40%

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Cyanobacteria for PHB Bioplastics Production: A Review

Markl¹,

Grünbichler²,

Lackner³

2019

Algae

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Cyanobacteria, or blue-green algae, can be used as host to produce polyhydroxyalkanoates (PHA), which are promising bioplastic raw materials. The most important material thereof is polyhydroxybutyrate (PHB), which can replace the commodity polymer polypropylene (PP) in many applications, yielding a bio-based, biodegradable alternative solution. The advantage from using cyanobacteria to make PHB over the standard fermentation processes, with sugar or other organic (waste) materials as feedstock, is that the sustainability is better (compare first-generation biofuels with the feed vs. fuel debate), with CO 2 being the only carbon source and sunlight being the sole energy source. In this review article, the state of the art of cyanobacterial PHB production and its outlook is discussed. Thirty-seven percent of dry cell weight of PHB could be obtained in 2018, which is getting close to up to 78% of PHB dry cell weight in heterotrophic microorganisms in fermentation reactors. A good potential for cyanobacterial PHB is seen throughout the literature.

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“…carbon source [27,28], which is significantly above the other reported values from literature. The strain had been subjected to UV light mutations to increase the PHB productivity.…”

Section: Phb Production By Cyanobacteria: Current State Of Knowledgementioning

confidence: 40%

“…Genetic engineering is commonly deployed to increase the yield of PHB compared to wild types [26,31,32]. Also, bioprocess optimization is carried out [27,28]. Growth is typically followed by nitrogen and/or phosphorous limitation.…”

Section: Phb Production By Cyanobacteria: Current State Of Knowledgementioning

confidence: 99%

Cyanobacteria for PHB Bioplastics Production: A Review

Markl¹,

Grünbichler²,

Lackner³

2019

Algae

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“…PHB has been envisaged as a replacement for PP for a considerable time, and now a process to avoid making PHB from feed and food raw had been developed. In this very study, that photosynthetically produced PHB by Kamravamanesh et al [14], was characterized, and the material was found to exhibit comparable properties to "traditional" PHB samples. Due to limited amounts of sample, no mechanical characterization could be performed, which could be the basis for further tests toward compound formulations and/or application testing.…”

Section: Discussionmentioning

confidence: 80%

“…In this study the polymer produced from the high PHB producing mutant of cyanobacterial strain Synechocystis sp. PCC 6714, MT_a24 [14] was extracted using liquidliquid extraction from the whole cells. The characterization of the polymer was done using various methods such as Fourier Transform Infrared spectroscopy (FTIR) for the chemical characterization whereas Differential Scanning Calorimetry (DSC) was used to determine the thermal properties.…”

Section: Introductionmentioning

confidence: 99%

Characterization of photosynthetically synthesized poly(3-hydroxybutyrate) using a randomly mutated strain of Synechocystis sp. PCC 6714

Lackner

Kamravamanesh

Krampl

et al. 2019

International Journal of Biobased Plastics

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Conventional polymers are made from fossil resources and consume fossil energy in their production, yielding CO 2 emissions. Production of large-volume compounds from CO 2 , either from point sources or from the air, could reduce anthropogenic global warming. Poly(3-hydroxybutyrate) (PHB) is a biodegradable and biobased thermoplastic polymer which can be synthesized sustainably using CO 2. PHB exhibits a high crystallinity and shows properties similar to synthetic polyesters and also to polyolefins such as polypropylene. However, the low elongation at break and the brittleness of PHB are limitations, which, however, can be overcome by using other polyhydroxyalkanoates (PHA) as blends or copolymers. In this study, PHB produced by cyanobacteria from CO 2 was extracted and characterized using various techniques. The Fourier Transform Infrared spectroscopy (FTIR) showed the characteristic signals expected for the phototrophic PHB. Differential Scanning Calorimetry (DSC) for thermal characterization of PHB showed a melting temperature of 151.42°C and 73.11°C for the crystallization process. From the Gel Permeation Chromatography (GPC) the molecular weight of cyanobacterial PHB was determined to be 1,051,900 g mol −1. The analytics results show that the photosynthetically produced PHB ressembles conventional PHB from sugar fermentation, which is an established biopolymer today. It can be concluded that photosynthetically produced PHB has a strong market potential, backed by its sustainable production technology.

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“…The authors previously showed the cyanobacterial strain MT_a24, a UV-mutated strain of Synechocystis sp. PCC 6714 produces PHB of up to 37 ± 4 % dry cell weight (DCW) under nitrogen and phosphorus limitation showing the highest productivity of 134 mg L −1 d −1 [6]. Under lab conditions, it was also shown that media optimization can be used to increase PHB content in MT_a24 to up to 1.16 g L −1 [7].…”

Section: Introductionmentioning

confidence: 99%