Increased polyethylene glycol-mediated fusion competence in mitotic cells of a mouse lymphoid cell line

Hansen, Donald; Stadler, Janice

doi:10.1007/bf01539119

Cited by 19 publications

(5 citation statements)

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“…On the basis of a previous report of increased polyethylene glycol (PEG)-mediated fusion competence in mitotic cells of a mouse lymphoid cell line, 12 the human HT1080 cells were first synchronized at the G1/S phase by using a double thymidine block, then were released and incubated for 8 h to induce entry into M phase. The mitotic cells were harvested and fused via PEG-mediated cell fusion with the protoplasts, according to the protocol developed in this study (Figure 1, see Methods).…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Maintenance and Function of a Plant Chromosome in Human Cells

Wada¹,

Kazuki²,

Kazuki³

et al. 2016

ACS Synth. Biol.

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Replication, segregation, gene expression, and inheritance are essential features of all eukaryotic chromosomes. To delineate the extent of conservation of chromosome functions between humans and plants during evolutionary history, we have generated the first human cell line containing an Arabidopsis chromosome. The Arabidopsis chromosome was mitotically stable in hybrid cells following cell division, and initially existed as a translocated chromosome. During culture, the translocated chromosomes then converted to two types of independent plant chromosomes without human DNA sequences, with reproducibility. One pair of localization signals of CENP-A, a marker of functional centromeres was detected in the Arabidopsis genomic region in independent plant chromosomes. These results suggest that the chromosome maintenance system was conserved between human and plants. Furthermore, the expression of plant endogenous genes was observed in the hybrid cells, implicating that the plant chromosomal region existed as euchromatin in a human cell background and the gene expression system is conserved between two organisms. The present study suggests that the essential chromosome functions are conserved between evolutionarily distinct organisms such as humans and plants. Systematic analyses of hybrid cells may lead to the production of a shuttle vector between animal and plant, and a platform for the genome writing.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Maintenance and Function of a Plant Chromosome in Human Cells

Wada¹,

Kazuki²,

Kazuki³

et al. 2016

ACS Synth. Biol.

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“…Chromosome preparation and identification methods were very similar to those of Farber and Davidson (9 (6,11). Stationary cells were diluted mm petri dish in to 2 x 105/ml and maintained at this concentra-' M ouabain and tion by daily dilution for 3 days.…”

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confidence: 99%

Regulation of the capacity for O6-methylguanine removal from DNA in human lymphoblastoid cells studied by cell hybridization.

Ayres¹,

Sklar²,

K³

et al. 1982

Mol. Cell. Biol.

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Hybrids were made between a ouabain-resistant, thioguanine-resistant human lymphoma line able to remove O6-methylguanine from its DNA (Mex+) and human lymphoblastoid lines deficient in this capability (Mex-). The formation of hybrids was confirmed by chromosomal analysis. Hybrid cells had an O6-methylguanine removal capacity per mole of guanine about one third to one half that of the Mex+ parents, i.e., about the same per cell. Cell hybrids removed the same amount of the alkylation adduct 3-methyladenine as did their parents per mole of guanine, i.e., about twice as much per cell. Although the cell hybrids had intermediate resistance to the cytotoxic action of N-methyl-N'-nitro-N-nitrosoguanidine used to induce O6-methylguanine and 3-methyladenine, there is evidence that the ability to remove O6-methylguanine and resistance to the cytotoxic effect of N-methyl-N'-nitro-N-nitrosoguanidine are dissociable characteristics.

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“…It is most probably due to rapid passage of G2 nuclei to pro phase. Eventhough mitotic cells are known to have higher affinity towards fusion than in terphase cells (Hansen and Stadler 1977), it is unlikely that high mitotic activity of polykaryons in our experiment is due to preferential fusion of mitotic cells. This is indicated by two facts.…”

Section: Resultsmentioning

confidence: 75%