Increased production of bacterial cellulose as starting point for scaled-up applications

Abstract

Bacterial cellulose is composed of an ultrafine nanofiber network and well-ordered structure; therefore, it offers several advantages when used as native polymer or in composite systems.In this study, a pool of 34 acetic acid bacteria strains belonging to Komagataeibacter xylinus were screened for their ability to produce bacterial cellulose. Bacterial cellulose layers of different thickness were observed for all the culture strains. A high-producing strain, which secreted more than 23 g/L of bacterial cellulose on the isolation broth during 10 days of static cultivation, was selected and tested in optimized culture conditions. In static conditions, the increase of cellulose yield and the reduction of by-products such as gluconic acid were observed. Dried bacterial cellulose obtained in the optimized broth was characterized to determine its microstructural, thermal, and mechanical properties. All the findings of this study support the use of bacterial cellulose produced by the selected strain for biomedical and food applications.

Production of high crystallinity type‐I cellulose from Komagataeibacter hansenii JR‐02 isolated from Kombucha tea

“…In order to improve the BC productivity of strain JR‐02 in shaking condition, we could try to screen the highest cellulose producing strain or add an appropriate amount of acetate buffer in media for maintaining the pH suitable for BC production or choose an optimum impeller speed for eliminating the Cel − mutants . In future research, the BC productivity of strain JR‐02 would be further improved by optimized design of media and fermentation conditions.…”

Influence of chemical and physical conditions in selection ofGluconacetobacter hanseniiATCC 23769 strains with high capacity to produce bacterial cellulose for application as sustained antimicrobial drug-release supports

“…), which allows its use in various fields, such as industrial, medical and pharmaceutical areas, healthy food and cosmetics (Gullo et al . ). Due to its physical and chemical properties, BC membrane has presented several applications in medicine, such as temporary artificial skin for burns and ulcers, dental implant components and as artificial blood vessels for microsurgery (Lustri et al .…”

“…According to Gullo et al . (), the yield of BC membrane is also affected by the culture conditions and carbon source variations.…”

Nanofibrillated bacterial cellulose and pectin edible films added with fruit purees

“…Bacterial cellulose (BC) is produced as a membrane (pellicle) by some bacteria species in a bottom-up route in which the bacteria synthesize cellulose and build up bundles of ribbon-shaped nanofibrils (Pecoraro, Manzani, Messaddeq, & Ribeiro, 2008). Komagataeibacter xylinus (formerly Gluconacetobacter xylinus) is considered as the highest producer and model organism in studies involving BC synthesis (Gullo et al, 2017). Although it has the same chemical composition as plant cellulose, BC is more pure (free from hemicellulose and lignin), which is useful to reduce purifying costs and environmental impacts (Duarte et al, 2015).…”