Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid

Sivanandhan, Ganeshan; Dev, Gnanajothi Kapil; Jeyaraj, Murugaraj; Rajesh, Manoharan; Annavi, Arjunan; Muthuselvam, Manickam; Manickavasagam, Markandan; Selvaraj, N.; Ganapathi, A.

doi:10.1007/s11240-013-0297-z

Cited by 139 publications

(54 citation statements)

References 35 publications

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“…SA with transgenic technology, highly enhanced the production of tanshinones in S. miltiorrhiza hairy roots [62]. Optimum production of withanolide A, withanone and withaferin A were reported in the elicited-hairy roots of Withania somnifera [63] . Jasmonates: Jasmonic acid has been proposed as key compounds of the signal transduction pathway involved in the elicitation of secondary metabolite biosynthesis which takes part in plant defense reactions [64].…”

Section: Hormonal Elicitorsmentioning

confidence: 99%

“…MeJA with transgenic technology, highly enhanced the production of tanshinones in S. miltiorrhiza hairy roots [62]. In the hairy root culture of W. somnifera, MeJA elicited the production of withanolide A, withanone and withaferin A [63]. The root cultures of Taverniera cuneifolia treated with different concentrations of MeJA, the glycyrrhizic acid content increased gradually with an increase in MeJA (1-100 μM) concentration.…”

Section: Hormonal Elicitorsmentioning

confidence: 99%

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Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review

Naik¹,

Al–Khayri²

2016

JARB

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Section: Hormonal Elicitorsmentioning

confidence: 99%

Section: Hormonal Elicitorsmentioning

confidence: 99%

Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review

Naik¹,

Al–Khayri²

2016

JARB

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“…These in turn regulate the expression of genes involved in plant secondary metabolism (Kim et al, 2009). Sivanandhan et al (2013) used methyl jasmonate and salicylic acid (SA) as elicitors for Withania somnifera hairy root cultures (Sivanandhan et al, 2013). Withaferin A content was 34-fold higher than control with the elicitation of methyl jasmonate and its production was 42-fold higher with SA elicitation.…”

Section: Elicitor Treatment For Increased Production Of Withanolidesmentioning

confidence: 99%

Withanolides: Strategies for Enhanced Production and Mechanistic Insights into their Mode of Actio

Grover¹

2015

Proceedings of the Indian National Science Academy

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A large number of withanolides are isolated from solanaceous plants. Around 360 naturally occurring withanolides have been identified till date. These compounds are found to have useful pharmacological activities, out of which, anticancer activities of withanolides have drawn considerable interest from researchers. As plants being their sole source, in which they are synthesized in considerably low amounts, biotechnological interventions have been explored in order to increase the yield of these secondary metabolites. Their molecular mechanisms of action also remained largely unexplored for a long time. This review covers recent attempts which have ben made to enhance the production of withanolides and discusses the studies that elucidated their prospective action mechanisms.

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“…The transgenic nature of hairy roots was confi rmed by PCR using npt II and rol B gene specifi c primers and transgenicity was also confi rmed by southern blot analysis. Sivanandhan et al [ 23 ] induced hairy roots by infecting the leaf explants from 45 day old in vitro seedlings with agropine type strain of A. rhizogenes R1000. Hairy roots induced by using A. rhizogenes stains R1601 and R1000 are found stable and could be used for optimization of cultural parameters for biomass and metabolite production.…”

Section: Induction Of Adventitious Rootsmentioning

confidence: 99%

Production of Withanolides from Cell and Organ Cultures of Withania somnifera (L.) Dunal

Nagella

Murthy

2014

Production of Biomass and Bioactive Compounds Using Bioreactor Technology

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Withania somnifera (L.) Dunal. (Indian ginseng) is one of the most important medicinal plants used as a crude drug for its preventive and therapeutic purposes. Among the diverse constituents of Withania , withanolides are found to be the major components responsible for their biological and pharmacological actions. On the other hand, diffi culty in supplying the pure withanolides in suffi cient quantity prevents the development of Withania for clinical medicines. Field cultivation of Withania is time consuming and it needs extensive efforts for quality control as the growth is susceptible to many environmental factors including soil, climate, pathogens and pests. To overcome these problems, cell and organ cultures have been widely explored for more rapid and effi cient production of Withania biomass and withanolides. Recently, cell and organ cultures of W. somnifera have been developed in laboratory scale with a view to establish large scale production using bioreactors. Various physical and chemical parameters affecting the biomass production and withanolide accumulation have been investigated. For commercial withanolide production, fi eld grown plant material has generally been used but the quality of these products may be highly affected by different environmental conditions, pollutants and pests and pathogens like insects, fungi, bacteria and viruses, which can result in a heavy loss in yield and alter the medicinal content of plant. Plant cell and organ cultures are promising technologies to obtain plant-specifi c valuable metabolites [ 9 ]. Cell and organ cultures have a higher rate of metabolism than fi eld grown plants because the initiation of cell and organ growth in culture leads to fast proliferation of cells/organs and to a condensed biosynthetic cycle. Further, plant cell/organ cultures are not limited by environmental, ecological and climatic conditions and cells/organs can thus proliferate at higher growth rates than whole plant in cultivation [ 10 ]. Keywords Induction of Adventitious RootsRani et al. [ 14 ] induced adventitious roots from leaf explants of Withania by dip method. The leaf explants were dipped in different concentrations of IBA for different intervals of time and cultured the explants on the MS medium of different strengths. Adventitious roots were induced directly when leaf explants were cultured on half strength MS medium supplemented with a combination of IBA and IAA at 9.85 and 2.85 μM respectively. Other auxin combinations resulted in the formation of callus along with adventitious root induction. The adventitious roots were cultured in a 2.5 L bubble column reactor with 1,000 mL of half strength MS medium with the same hormonal concentration for 6 weeks and the roots were harvested and analyzed for the withanolide production. A maximum yield of 10 mg g ) for the induction of adventitious roots. The explants cultured on full strength MS medium produced only callus in all the concentrations tested, which hindered the induction of roots. Explants cultured on half MS m...

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Increased production of withanolide A, withanone, and withaferin A in hairy root cultures of Withania somnifera (L.) Dunal elicited with methyl jasmonate and salicylic acid

Cited by 139 publications

References 35 publications

Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review

Impact of Abiotic Elicitors on In vitro Production of Plant Secondary Metabolites: A Review

Withanolides: Strategies for Enhanced Production and Mechanistic Insights into their Mode of Actio

Production of Withanolides from Cell and Organ Cultures of Withania somnifera (L.) Dunal

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