Increased proportion of medium chain fatty acids in nystatin‐resistant yeast mutants

Abstract

Fatty acid composition of phospholipids and steryl esters from four nystatin-resistant mutants of Saccharomyces cerevisiae was compared to that from the wild strain. All the mutant strains which produce several ergosterol intermediates incorporated two- to three-fold as much medium chain fatty acids, especially 14:1 in phospholipids, and 12:0, 14:0 and 14:1 in steryl esters as the wild strain did. The increase in the relative amount of medium chain fatty acids in these mutants was found at all the growth temperatures and the growth phases examined, and in all the phospholipid species.

Sterol methylation in Saccharomyces cerevisiae

“…Yeast sterol mutants have also been investigated for phospholipid alterations. Small changes were seen in Candida albicans and in S. cerevisiae nystatin-resistant mutants (17,25), but the defects in sterol synthesis were not defined.…”

The interaction between lipid peroxidation and prostaglandin synthesis in rabbit kidney-medulla slices

“…The inhibition of malondialdehyde formation by mepacrine in our experiments might be due to the following factors. (1) Mepacrine might protect unsaturated fatty acids from initiation and propagation of lipid peroxidation by binding to phospholipids in the membranes (Nagai et al, 1981), thereby preventing the formation of malondialdehyde from linear hydroperoxides of fatty acids.…”