Increased proto‐oncogene expression in peripheral blood T lymphocytes from patients with systemic sclerosis

Kahan, A; Gerfaux, Jacqueline; Kahan, André; Joret, Anne‐Marie; Menkès, C J

doi:10.1002/anr.1780320412

Cited by 24 publications

(7 citation statements)

References 30 publications

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“…Since protein binding to the NF-AT site was enhanced, it might be assumed that increased expression of the common NF-KB is related to activated T cells in SSc as well. Consistent with enhanced NF-KB and NF-AT during T cell activation, increased c-ras expression has been demonstrated in SSc T cells [28]. However, since we studied mixed cell populations, influences by B cells and monocytes on detection of DNA-binding proteins have to be discussed.…”

Section: Discussionsupporting

confidence: 54%

Analysis of proteins that interact with the IL-2 regulatory region in patients with rheumatic diseases

Becker

Stengl

Stein

et al. 1995

Clinical and Experimental Immunology

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SUMMARY In order to investigate transcriptional regulation of lymphokine genes in rheumatic diseases, peripheral blood mononuclear cells from patients with systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), and systemic sclerosis (SSc) were analysed for expression of DNA‐binding proteins. Nuclear extracts prepared from unstimulated and mitogen‐activated cells were studied for their ability to bind to 32P‐labelled oligonucleotides containing the AP‐1, NF‐AT, NF‐B and CD28RC sites of the IL‐2 promoter. Using gel mobility‐shift assay, detection of protein binding to the AP‐1 site was reduced in SLE compared with controls. NF‐AT binding activity was enhanced in all groups of patients, and was associated with measures of disease activity in RA. In addition. SSc patients showed increased NF‐×B binding activity. Altered patterns of DNA‐binding proteins suggest disturbed intracellular signalling which may contribute to abnormal lymphokine production in rheumatic diseases.

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Section: Discussionsupporting

confidence: 54%

Analysis of proteins that interact with the IL-2 regulatory region in patients with rheumatic diseases

Becker

Stengl

Stein

et al. 1995

Clinical and Experimental Immunology

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“…Increased protooncogene expression (42) and an increased frequency of hypoxanthine guanine phosphoribosyltransferase gene-mutated T cells (43), both of which are suggestive of T cell activation, were observed in patients with SSc. Increased levels of soluble IL-2R were also found in suction blister fluid from the skin of patients with SSc (44).…”

Section: T Cells In Sscmentioning

confidence: 98%

Is systemic sclerosis an antigen‐driven T cell disease?

Sakkas¹,

Platsoucas²

2004

Arthritis & Rheumatism

113

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“…T cells are also found in lung tissues from SSc patients with lung involvement (8). Peripheral blood T cells in SSc exhibit signs of activation and express activation Ags, such as IL-2R (CD25), HLA-DR, and CD29 (9,10), and increased levels of protooncogenes (11). Increased levels of soluble IL-2R were found in suction blister fluid in the skin of patients with SSc (12).…”

Section: S Ystemic Sclerosis (Ssc)mentioning

confidence: 99%

Oligoclonal T Cell Expansion in the Skin of Patients with Systemic Sclerosis

Sakkas

Artlett

et al. 2002

The Journal of Immunology

194

169

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Fibrosis, microvascular fibroproliferative alterations, and autoantibody production are the main features of systemic sclerosis (SSc), and all of them can be explained by cytokine production by activated T cells. However, little is known about the role of T cells in the pathogenesis of SSc, and there is no information on the Ag(s) that elicits such activation. To determine whether T cells infiltrating the skin biopsies of patients with SSc are oligoclonal, β-chain TCR transcripts from T cells infiltrating the skin of five patients with SSc of recent onset were amplified by either Vβ-specific PCR or nonpalindromic adaptor PCR. The resulting PCR products were subsequently cloned and sequenced. High proportions of identical β-chain TCR transcripts ranging from 43 to 90% of those sequenced were found in five patients, strongly suggesting the presence of oligoclonal T cells in these infiltrates. A dominant T cell clone was found to be clonally expanded in skin biopsies obtained from a single patient with SSc at three different times (0, 8, and 13 mo earlier) and from three different skin regions. β-chain TCR transcripts from PBMC from normal donors (methodological control) were unique when compared with each other, typical for polyclonal populations of T cells. The finding of oligoclonal T cells infiltrating the skin of patients with SSc suggests that these T cells have undergone proliferation in situ in the skin and clonal expansion in response to as yet unidentified Ag(s). These results suggest that T cells are involved in the pathogenesis of the disease.

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Increased proto‐oncogene expression in peripheral blood T lymphocytes from patients with systemic sclerosis

Cited by 24 publications

References 30 publications

Analysis of proteins that interact with the IL-2 regulatory region in patients with rheumatic diseases

Analysis of proteins that interact with the IL-2 regulatory region in patients with rheumatic diseases

Is systemic sclerosis an antigen‐driven T cell disease?

Oligoclonal T Cell Expansion in the Skin of Patients with Systemic Sclerosis

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