Increased Relative Abundance of Klebsiella pneumoniae Carbapenemase-producing Klebsiella pneumoniae Within the Gut Microbiota Is Associated With Risk of Bloodstream Infection in Long-term Acute Care Hospital Patients

Shimasaki, Teppei; Seekatz, Anna M.; Bassis, Christine M.; Rhee, Yoona; Yelin, Rachel D; Fogg, Louis; Dangana, Thelma; Cornejo, E; Weinstein, Robert A.; Okamoto, Koh; Lolans, Karen; Schoeny, Michael; Lin, Michael; Moore, Nicholas; Young, Vincent B.; Hayden, Mary K.; Program, Prevention Epicenters

doi:10.1093/cid/ciy796

Cited by 99 publications

(85 citation statements)

References 32 publications

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“…In prospective studies, these procedures are time consuming and can not be applied in numerous labs. Quantitative studies with other bacteria are very rare, based on total microbiota analyses or absent and these studies are needed (11, 12). To have the opportunity to freeze directly available devices could be useful in many labs and increase the feasibility of these experiments.…”

Section: Introductionmentioning

confidence: 99%

Aerobic bacteria as Escherichia coli can survive in ESwab^TM medium after a 3 month-freezing at −80°C but not after multiple thawing

Saliba

Carbonnelle

Lescat

2019

Preprint

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14Pretesting of procedures for specimen conservation should be part of preliminary 15 studies for trials especially when quantification of bacteria must be performed in a second 16 time. Quantitative epidemiological studies of multidrug resistant organisms sampled from 17 rectal swabs could be then particularly favored. The aim of this preliminary study, was to 18 evaluate the performance of the flocked swab with liquefied Amies transport medium 19 ESwab TM , for the survival evaluation of aerobic bacteria, from rectal swab sampling 20 42 (5). Likewise, those species are also opportunistic pathogens and quantitative studies of the 43 prevalence, bacterial load and diversity of these species as well as E. coli in their natural 44 3 environment, which is the digestive gut, are essential (3, 6, 7). 45 Several steps in the analyses of bacteria from digestive gut are crucials and are directly 46 related to a good diagnostic performance in the Clinical Microbiology laboratory. For 47

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Section: Introductionmentioning

confidence: 99%

Aerobic bacteria as Escherichia coli can survive in ESwab^TM medium after a 3 month-freezing at −80°C but not after multiple thawing

Saliba

Carbonnelle

Lescat

2019

Preprint

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“…Although several studies have identified CRE colonization as a risk factor for subsequent CRE infection (6,7), available investigations do not explore whether this risk differs based on the production of carbapenemases by the colonizing strain. This information is important because it can guide whether the additional step of performing phenotypic or genotypic testing on rectal isolates positive for CRE provides added value.…”

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confidence: 99%

The Likelihood of Developing a Carbapenem-Resistant Enterobacteriaceae Infection during a Hospital Stay

Kazmi

Bergman

Goodman

et al. 2019

Antimicrob Agents Chemother

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Of 1,455 unique patients in U.S. intensive care units (ICUs), 4% were rectally colonized with CRE on admission. A total of 297 patients were initially negative for carbapenem-resistant Enterobacteriaceae (CRE) and remained in the ICU long enough to contribute additional swabs; 22% of these patients had a subsequent CRE-positive swab, with a median time to CRE colonization of 13 days (interquartile range, 7 to 21 days). Patients colonized with carbapenemase-producing CRE were more likely than those colonized with non-carbapenemase-producing CRE to develop CRE infections during their hospitalizations (36% versus 3%; P Ͻ 0.05).

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“…Therefore, decreasing the intestinal loads of OXA-PE might be an effective way to reduce the risk of cross-transmission in wards harboring colonized patients. Moreover, the decrease of the OXA-PE loads below the detection limits can also reduce the risk of developing an infections during hospitalization [20][21][22]. This could be useful in situations where programmed surgical procedures are to be performed and for managing critically ill patients, especially since the decrease of the OXA-PE loads after the onset of treatment was fast and maintained throughout the treatment.…”

Section: Discussionmentioning

confidence: 99%

Proof-of-concept trial of the combination of lactitol with Bifidobacterium bifidum and Lactobacillus acidophilus for the eradication of intestinal OXA-48-producing Enterobacteriaceae

et al. 2020

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Background: The major reservoir of carbapenemase-producing Enterobacteriaceae (CPE) is the gastrointestinal tract of colonized patients. Colonization is silent and may last for months, but the risk of infection by CPE in colonized patients is significant. Methods: Eight long-term intestinal carriers of OXA-48-producing Enterobacteriaceae (OXA-PE) were treated during 3 weeks with daily oral lactitol (Emportal ®), Bifidobacterium bifidum and Lactobacillus acidophilus (Infloran ®). Weekly stool samples were collected during the treatment period and 6 weeks later. The presence of OXA-PE was investigated by microbiological cultures and qPCR. Results: At the end of treatment (EoT, secondary endpoint 1), four of the subjects had negative OXA-PE cultures. Three weeks later (secondary endpoint 2), six subjects were negative. Six weeks after the EoT (primary endpoint), three subjects had negative OXA-PE cultures. The relative intestinal load of OXA-PE decreased in all the patients during treatment. Conclusions: The combination of prebiotics and probiotics was well tolerated. A rapid reduction on the OXA-PE intestinal loads was observed. At the EoT, decolonization was achieved in three patients.

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Increased Relative Abundance of Klebsiella pneumoniae Carbapenemase-producing Klebsiella pneumoniae Within the Gut Microbiota Is Associated With Risk of Bloodstream Infection in Long-term Acute Care Hospital Patients

Cited by 99 publications

References 32 publications

Aerobic bacteria as Escherichia coli can survive in ESwab^TM medium after a 3 month-freezing at −80°C but not after multiple thawing

Aerobic bacteria as Escherichia coli can survive in ESwab^TM medium after a 3 month-freezing at −80°C but not after multiple thawing

The Likelihood of Developing a Carbapenem-Resistant Enterobacteriaceae Infection during a Hospital Stay

Proof-of-concept trial of the combination of lactitol with Bifidobacterium bifidum and Lactobacillus acidophilus for the eradication of intestinal OXA-48-producing Enterobacteriaceae

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Increased Relative Abundance of Klebsiella pneumoniae Carbapenemase-producing Klebsiella pneumoniae Within the Gut Microbiota Is Associated With Risk of Bloodstream Infection in Long-term Acute Care Hospital Patients

Cited by 99 publications

References 32 publications

Aerobic bacteria as Escherichia coli can survive in ESwabTM medium after a 3 month-freezing at −80°C but not after multiple thawing

Aerobic bacteria as Escherichia coli can survive in ESwabTM medium after a 3 month-freezing at −80°C but not after multiple thawing

The Likelihood of Developing a Carbapenem-Resistant Enterobacteriaceae Infection during a Hospital Stay

Proof-of-concept trial of the combination of lactitol with Bifidobacterium bifidum and Lactobacillus acidophilus for the eradication of intestinal OXA-48-producing Enterobacteriaceae

Contact Info

Product

Resources

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Aerobic bacteria as Escherichia coli can survive in ESwab^TM medium after a 3 month-freezing at −80°C but not after multiple thawing

Aerobic bacteria as Escherichia coli can survive in ESwab^TM medium after a 3 month-freezing at −80°C but not after multiple thawing