Increased removal of cadmium by <i>Chlamydomonas reinhardtii</i> modified with a synthetic gene for γ-glutamylcysteine synthetase

Piña-Olavide, René; Paz-Maldonado, Luz María Teresita; Torre, Maria Catalina Alfaro-De la; García-Soto, Mariano J.; Ramírez-Rodríguez, Angélica E; Rosales‐Mendoza, Sergio; Bañuelos-Hernández, Bernardo; la-Cruz, R F Garcia De

doi:10.1080/15226514.2020.1765138

Cited by 27 publications

(4 citation statements)

References 44 publications

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“…Two GSH biosynthesis enzymes, γ-glutamylcysteine synthetase (encoded by gshI ) and glutathione synthetase (encoded by gshII ) from E. coli , were overexpressed in Brassica juncea ; this manipulation led to a 25% increase in Cd uptake in transgenic plants versus wild type [ 138 ]. Recently, overexpression of a synthetic gene ( gshA ) encoding for γ-glutamylcysteine synthetase was shown to significantly increase Cd tolerance of C. reinhardtii [ 138 , 139 ]. Furthermore, the CrGNAT gene, encoding an acetyltransferase involved in histone methylation and chromatin remodeling, was overexpressed in C. reinhardtii ; under toxic concentrations of Cu, a marked increase in cell population, chlorophyll accumulation, and photosynthesis efficiency was observed compared to wild type, while CrGNAT knockdown lines with antisense exhibited sensitivity to Cu stress.…”

Section: Genetic Engineering Targets To Improve Microalgal Hm Bioreme...mentioning

confidence: 99%

Is Genetic Engineering a Route to Enhance Microalgae-Mediated Bioremediation of Heavy Metal-Containing Effluents?

Ranjbar

Malcata

2022

Molecules

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Contamination of the biosphere by heavy metals has been rising, due to accelerated anthropogenic activities, and is nowadays, a matter of serious global concern. Removal of such inorganic pollutants from aquatic environments via biological processes has earned great popularity, for its cost-effectiveness and high efficiency, compared to conventional physicochemical methods. Among candidate organisms, microalgae offer several competitive advantages; phycoremediation has even been claimed as the next generation of wastewater treatment technologies. Furthermore, integration of microalgae-mediated wastewater treatment and bioenergy production adds favorably to the economic feasibility of the former process—with energy security coming along with environmental sustainability. However, poor biomass productivity under abiotic stress conditions has hindered the large-scale deployment of microalgae. Recent advances encompassing molecular tools for genome editing, together with the advent of multiomics technologies and computational approaches, have permitted the design of tailor-made microalgal cell factories, which encompass multiple beneficial traits, while circumventing those associated with the bioaccumulation of unfavorable chemicals. Previous studies unfolded several routes through which genetic engineering-mediated improvements appear feasible (encompassing sequestration/uptake capacity and specificity for heavy metals); they can be categorized as metal transportation, chelation, or biotransformation, with regulation of metal- and oxidative stress response, as well as cell surface engineering playing a crucial role therein. This review covers the state-of-the-art metal stress mitigation mechanisms prevalent in microalgae, and discusses putative and tested metabolic engineering approaches, aimed at further improvement of those biological processes. Finally, current research gaps and future prospects arising from use of transgenic microalgae for heavy metal phycoremediation are reviewed.

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Section: Genetic Engineering Targets To Improve Microalgal Hm Bioreme...mentioning

confidence: 99%

Is Genetic Engineering a Route to Enhance Microalgae-Mediated Bioremediation of Heavy Metal-Containing Effluents?

Ranjbar

Malcata

2022

Molecules

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“…As previously noted in the section (2.2.2), the biosynthesis of GSH occurs via an enzymatic reaction catalyzed by γ -GCS. Recently, Piña-olavide et al. (2020) reported that the transformation of C. reinhardtii with a synthetic gene (gshA) encoding for γ-GCS had significantly increased Cd (II) bioaccumulation.…”

Section: Future Outlook For Enhancing the Phycoremediation Processmentioning

confidence: 99%

Phycoremediation mechanisms of heavy metals using living green microalgae: physicochemical and molecular approaches for enhancing selectivity and removal capacity

Danouche

Ghachtouli

Arroussi

2021

Heliyon

140

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Heavy metal (HM) contamination of water bodies is a serious global environmental problem. Because they are not biodegradable, they can accumulate in food chains, causing various signs of toxicity to exposed organisms, including humans. Due to its effectiveness, low cost, and ecological aspect, phycoremediation, or the use of microalgae's ecological functions in the treatment of HMs contaminated wastewater, is one of the most recommended processes. This study aims to examine in depth the mechanisms involved in the phycoremediation of HMs by microalgae, it also provides an overview of the prospects for improving the productivity, selectivity, and cost-effectiveness of this bioprocess through physicochemical and genetic engineering applications. Firstly, this review proposes a detailed examination of the biosorption interactions between cell wall functional groups and HMs, and their complexation with extracellular polymeric substances released by microalgae in the extracellular environment under stress conditions. Subsequently, the metal transporters involved in the intracellular bioaccumulation of HMs as well as the main intracellular mechanisms including compartmentalization in cell organelles, enzymatic biotransformation, or photoreduction of HMs were also extensively reviewed. In the last section, future perspectives of physicochemical and genetic approaches that could be used to improve the phytoremediation process in terms of removal efficiency, selectivity for a targeted metal, or reduction of treatment time and cost are discussed, which paves the way for large-scale application of phytoremediation processes.

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“…This phenomenon elucidated increased metal binding, rather than metal transit, was the primary cause of increased metal bioaccumulation. Recently, Piña-olavide et al [65] reported that the transgenic C. reinhardtii carrying a synthetic gene (gshA) encoding for γ-GCS had significantly increased Cd (II) bioaccumulation.…”

Section: Transgenic Microalgaementioning

confidence: 99%

Emerging role of microalgae in heavy metal bioremediation

et al. 2021

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Microalgae have been publicized for their diversified dominance responsiveness and bioaccumulation potential toward pollutants in an ecosystem. Also, algal's incredible capability as biocatalysts in environmental appliances has been well elucidated owing to their robustness and simple nutritional demand. Additionally, microalgae can deliver various collections of bio-based chemical compounds helpful for diversified applications, especially as green alternatives. The environment has been contaminated with various polluting agents; one principal polluting agent is heavy metals which are carcinogenic and show toxicity even in minimal quantity, cause unsatisfactory threats to the environmental ecosystem, including human and animal health. There is a prominent tendency to apply microalgae in the phytoremediation of heavy metals compounds because of its vast benefits, including great accessibility, cost-effective, excellent toxic metal eliminating efficiency, and nontoxic to the ecosystem. This review uncovers the most recent advancements and mechanisms associated with the bioremediation process and biosorption interaction of substantial harmful synthetic compounds processing microalgae species. Furthermore, future challenges and prospects in the utilization of microalgae in heavy metals bioremediation are also explored. The current review aims to give valuable information to aid the advancement of robust and proficient future microalgae-based heavy metal bioremediation innovations and summarizing a wide range of benefits socioeconomic scope to be employed in heavy metal compound removal in environment system.

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Increased removal of cadmium by Chlamydomonas reinhardtii modified with a synthetic gene for γ-glutamylcysteine synthetase

Cited by 27 publications

References 44 publications

Is Genetic Engineering a Route to Enhance Microalgae-Mediated Bioremediation of Heavy Metal-Containing Effluents?

Is Genetic Engineering a Route to Enhance Microalgae-Mediated Bioremediation of Heavy Metal-Containing Effluents?

Phycoremediation mechanisms of heavy metals using living green microalgae: physicochemical and molecular approaches for enhancing selectivity and removal capacity

Emerging role of microalgae in heavy metal bioremediation

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