Increased resistance against storage rot in transgenic carrots expressing chitinase  chit42  from  Trichoderma harzianum

Ojaghian, Seyedmohammadreza; Xie, Guishui; Zhang, Jingze

doi:10.1016/j.scienta.2018.02.025

Cited by 15 publications

(14 citation statements)

References 39 publications

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“…A previous research has reported increased resistance against carrot rot in transgenic carrots expressing chitinase chit42 from Trichoderma harzianum. 43…”

Section: Discussionmentioning

confidence: 99%

Resistance Induction Against White Rot of Tuber Mustard Using Chitosans and Acetyl Salicylic Acid

Ojaghian

Xie

2019

J Apple Biotechnol Rep

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Introduction: White rot, caused by Sclerotinia sclerotiorum, has recently become a serious threat to tuber mustard cultivation in Hangzhou, China. The objective of this study was to evaluate the inhibitory effect of acetyl salicylic acid (ASA) and three different chitosans (A, B and C) against mustard white rot. The degree of N-deacetylation and the molecular weight of chitosans A, B and C were 85%-1129 kDa, 95%-521 kDa and 75%-607 kDa, respectively. Materials and Methods: The inhibitory effect of chitosans with different concentrations against the mycelia growth and sclerotia formation of 3 isolates of the pathogen was determined in vitro. In addition, the efficacy of chitosans and ASA against mustard white rot was assessed during in vivo tests. After protein extraction, effects of chitosans and ASA on resistance related enzymes including chitinase, β-1,3-glucanase, phenylalanine ammonia lyase, polyphenol oxidase (PPO) and peroxidase (POD) were evaluated. Results: The chitosans reduced the mycelia growth and sclerotia formation of the pathogen. The chitosans showed significant antifungal effect against the disease in vivo. The chitosans and ASA markedly reduced the severity of the disease over time. Moreover, the chitosans and ASA markedly enhanced the level of most of the resistant related enzymes after 3 and 6 days. The chitosan B was found to have the best effect against tested pathogen isolates. Conclusions: The chitosan with the lowest molecular weight was found to be more effective against the disease. In addition, chitosans and ASA were able to significantly increase resistance-related enzymes over time indicating that they can be considered as resistant inducers against mustard white rot.

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“…A previous research has reported increased resistance against carrot rot in transgenic carrots expressing chitinase chit42 from Trichoderma harzianum. 43…”

Section: Discussionmentioning

confidence: 99%

Resistance Induction Against White Rot of Tuber Mustard Using Chitosans and Acetyl Salicylic Acid

Ojaghian

Xie

2019

J Apple Biotechnol Rep

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“…After disinfection in 2% hypochlorite sodium for 2 min, the seeds were washed with autoclaved tap water and dried on sterile filter papers. The seeds were sown in each plastic pot filled with field soil pasteurized at 75 ± 5 C for 1 h, topped with 1 cm of vermiculite and were watered as needed for 70 days (Ojaghian et al, 2018). The mustard plants were sprayed with the extracts dissolved in SDW at concentrations 0.5, 1 and 2 g/l using sterile medical syringes, so that the leaves were coated all over.…”

Section: Efficacy Of Crude Plant Extracts Against Mycelial Growthmentioning

confidence: 99%

“…Furthermore, PAL is an essential enzyme in the phenyl propanoid biosynthesis pathway leading to the synthesis of phytoalexins or phenols, which have defense functions in plants, such as fortification of the plant cell wall (Ojaghian et al, 2014). There are many studies showing a close association between the resistance against plant pathogens and expression of the resistance related genes (Métraux et al, 1991;McDowell and Woffenden, 2003;Ojaghian et al, 2018). This study showed that application of ethanol extracts of neem and ginger are able to significantly increase resistance related enzymes over time indicating that these extracts can be considered as resistance inducers against mustard white mold.…”

Section: Evaluation Of Pal Ppo and Podmentioning

confidence: 99%

Inhibitory Effect of Crude Extracts Derived From Aromatic Plants Against White Mold of Brassica Juncea Var. Tumida

Ojaghian

Saremi

Pashaei

2019

Pak. J. Phytopathol.

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The objective of this study was to evaluate antifungal activity and resistance inducing potential of crude extracts derived from neem (Azadirachta indica) and ginger (Zingiber officinale) against three isolates of Sclerotinia sclerotiorum, the causal agent of mustard white mold under in vitro and in vivo conditions. In addition, enzymatic tests were carried out to assess the effect of crude extracts on activities of resistance-inducing enzymes in mustard leaves. The results showed that ethanol extracts of neem and ginger at concentration 2 g/l were able to reduce mycelial growth of the pathogen (isolate 3) by 61.5 and 44.3%, respectively. The ethanol extracts of neem and ginger at concentration 2 g/l reduced infection radius on plant leaves from 9.7 in control to 3.1 and 3.4, respectively, due to antifungal efficacy. In addition, ethanol extracts of neem and ginger at concentration 2 g/l decreased infection radius (isolate 1) on plant leaves from 9.5 in control to 2.1 and 2.3, respectively, seven days after application. Enzymatic analyses showed significant increase in level of chitinases, β-1,3-glucanase, Phenylalanine ammonia lyase and Peroxidase due to application of ethanol extracts of neem and ginger.

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“… Baranski et al (2008) showed that the gene chit36 from Trichoderma harzianum enhances resistance of transformed carrot ( Daucus carota L.) to Alternaria radicina and Botrytis cinerea . Furthermore, introducing chimeric chit42 from T. harzianum to carrot markedly reduced the pathogenicity of S. sclerotiorum ( Ojaghian et al, 2018 ).…”

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confidence: 99%

“…The binary plasmid pGisPEC1 ( Fig. 1 ) used in this study was developed by Ojaghian et al (2018) . Disinfection of the seeds of tuber mustard was done using ethanol (15%) and then 0.2% (v/v) hypochlorite sodium for 2 min.…”

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confidence: 99%

Effect of Introducing Chitinase Gene on the Resistance of Tuber Mustard against White Mold

Ojaghian

Xie

2020

Plant Pathol J

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Increased resistance against storage rot in transgenic carrots expressing chitinase chit42 from Trichoderma harzianum

Cited by 15 publications

References 39 publications

Resistance Induction Against White Rot of Tuber Mustard Using Chitosans and Acetyl Salicylic Acid

Resistance Induction Against White Rot of Tuber Mustard Using Chitosans and Acetyl Salicylic Acid

Inhibitory Effect of Crude Extracts Derived From Aromatic Plants Against White Mold of Brassica Juncea Var. Tumida

Effect of Introducing Chitinase Gene on the Resistance of Tuber Mustard against White Mold

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