Increased respiratory burst activity of monocytes and polymorphonuclear leukocytes in psoriasis

Abstract

We studied the respiratory burst activity of peripheral blood monocytes and polymorphonuclear leukocytes (PMN) in eleven patients with psoriasis and eleven healthy controls, using zymosan particles to activate the phagocytes in vitro. The activation of the phagocytes was measured in a luminol-enhanced chiluminescence (CL) assay. We found a significantly higher respiratory burst activity of both monocytes and PMN in patients with psoriasis compared with the control subjects. The percentages of monocytes in psoriatics did not differ from those of the controls. The higher amount of zymosan-induced CL activity generated in the patient group was unrelated to the age of the individual. Our data indicate increased metabolic reactivity of both monocytes and PMN in psoriasis.

Functional Studies of Sessile Macrophages in Liver and Spleen of Psoriatics

“…Exper imental studies revealed close correlations between the central bone marrow pool, the monocyte system, and the tissue-fixed mac rophages. Therefore, results obtained in studying monocytes are comparable to those obtained in tissue-fixed macrophages [6], Signs of activation of monocytes, i.e., macro phages of blood, could be detected by in vitro experiments [3,16,24], Using a skilful in vi vo test system developed by Munz et al [19][20][21][22], we tried to find out if there is any functional alteration of tissue-fixed macro phages in psoriatics.…”

“…According to the thesis of van Furth [6] that tissue-fixed macrophages and blood monocytes belong to the unitary mononuclear phagocyte sys tem, investigations on sessile macrophages and blood monocytes are comparable. A nonspecific activation of blood monocytes in psoriatics could be revealed in vitro [3,16,24]. Polymorphonuclear leukocytes were found to be activated as well: increased chemotaxis [5,14,17,26], phagocytosis [26] and respiratory burst-activity [24].…”

“…A nonspecific activation of blood monocytes in psoriatics could be revealed in vitro [3,16,24]. Polymorphonuclear leukocytes were found to be activated as well: increased chemotaxis [5,14,17,26], phagocytosis [26] and respiratory burst-activity [24].…”

Increased Superoxide Anion Production in Dermal Fibroblasts of Psoriatic Patients

“…We were inter ested in studying the production of superoxide anion by psoriatic and normal dermal fibro blasts since in psoriasis the respiratory burst activity of monocytes and polymorphonuclear (PMN) leukocytes is increased [12], and acti vated PMN neutrophilic granulocytes play an important role in certain inflammatory skin disorders, particularly in psoriasis [13,14], The metabolic activation of PMN is associated with the release of reactive oxygen species (ROS), such as superoxide anion (CL-), hydro gen peroxide (FLCL), hydroxyl radical (OH) and singlet oxygen [15]. The exact cellular mechanisms involved in the production of ROS by PMN are not fully understood; how ever, there are certain features which allow an insight into complex processes [ 16].…”

Effect of μ, δ and κ Opioid Receptor Agonists on a Reactive Oxygen Species Mediated Model of Skin Inflammation

“…Tissue damage caused by reactive oxygen species (ROS) such as EECU and Oj" has been implicated in the pathogenesis of a number of inflammatory diseases including rheumatoid arthritis [1], psoriasis [2] and some forms of dermatitis [3,4], In rheumatoid arthritis ROS are formed during hypoxia-reperfusion cycles [5], which also occur in heart and kidney transplantation [6]. It has been reported [7] that y-hydroxybutyrate protects the intestine of the hamster from ROS-initiated tissue damage induced after 30 min of hypoxia, fol lowed by 3 h of reperfusion.…”