Increased Retinal Ganglion Cell Survival by Exogenous IL-2 Depends on IL-10, Dopamine D1 Receptors, and Classical IL-2/IL-2R Signaling Pathways

Colares, Tamiris Gago; Figueiredo, Camila Saggioro de; Souza, Lucienne de Oliveira Jesus; Santos, Aline Araújo dos; Giestal-de-Araujo, Elizabeth

doi:10.1007/s11064-021-03313-1

Cited by 7 publications

(3 citation statements)

References 82 publications

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“…Lysates were centrifuged at 10,000× g for 10 min at 4 °C, and the protein concentration was determined using a BCA assay. Western blot procedures were performed as described previously [ 23 ]. Samples with 30 μg protein per lane were resolved in 8–10% SDS-PAGE gels and blotted onto nitrocellulose membranes using a semi-dry blotting system (Bio-Rad Laboratories, Hercules, CA, USA).…”

Section: Methodsmentioning

confidence: 99%

Ouabain Effects on Human Anaplastic Thyroid Carcinoma 8505C Cells

Teixeira

Haddad

Passos

et al. 2022

Cancers

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Anaplastic thyroid carcinoma (ATC) is a rare, but aggressive, carcinoma derived from follicular cells. While conventional treatments may improve patients’ survival, the lethality remains high. Therefore, there is an urgent need for more effective ATC treatments. Cardiotonic steroids, such as ouabain, have been shown to have therapeutic potential in cancer treatment. Thus, we aimed to evaluate ouabain’s effects in human anaplastic thyroid cells. For this, 8505C cells were cultured in the presence or absence of ouabain. Viability, cell death, cell cycle, colony formation and migratory ability were evaluated in ouabain-treated and control 8505C cells. The expression of differentiation and epithelial-to-mesenchymal transition (EMT) markers, as well as IL-6, TGFb1 and their respective receptors were also quantified in these same cells. Our results showed that ouabain in vitro decreased the number of viable 8505C cells, possibly due to an inhibition of proliferation. A reduction in migration was also observed in ouabain-treated 8505C cells. In contrast, decreased mRNA levels of PAX8 and TTF1 differentiation markers and increased levels of the N-cadherin EMT marker, as well as IL-6 and TGFb1, were found in ouabain-treated 8505C cells. In short, ouabain may have anti-proliferative and anti-migratory effect on 8505C cells, but maintains an aggressive and undifferentiated profile.

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Section: Methodsmentioning

confidence: 99%

Ouabain Effects on Human Anaplastic Thyroid Carcinoma 8505C Cells

Teixeira

Haddad

Passos

et al. 2022

Cancers

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“…The mechanism by which cAMP exerts a protective effect on neurite outgrowth is also poorly understood [ 78 ]. Interleukin II [ 82 ], stimulation of CLIC1 channel expression [ 83 ], stromal cell-derived factor 1 (SDF1), and oncomodulin Ocm [ 84 ] have been implicated in this process. High levels of cAMP also promote retinal ganglion cell survival in mice [ 79 , 84 , 85 ] and rats [ 78 ] but lead to photoreceptor death [ 5 ] through a mechanism involving neogenin activation [ 86 ].…”

Section: Targets Of Camp Regulationmentioning

confidence: 99%

Multiple Roles of cAMP in Vertebrate Retina

Erofeeva

Meshalkina

Firsov

2023

Cells

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cAMP is a key regulatory molecule that controls many important processes in the retina, including phototransduction, cell development and death, growth of neural processes, intercellular contacts, retinomotor effects, and so forth. The total content of cAMP changes in the retina in a circadian manner following the natural light cycle, but it also shows local and even divergent changes in faster time scales in response to local and transient changes in the light environment. Changes in cAMP might also manifest or cause various pathological processes in virtually all cellular components of the retina. Here we review the current state of knowledge and understanding of the regulatory mechanisms by which cAMP influences the physiological processes that occur in various retinal cells.

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“…Some cytokines have been shown in previous studies to be related to elevated IOP and glaucomatous neuropathy [4][5][6][7]. Interleukin-2 (IL-2) can increase RGC survival after optic nerve axotomy [8]. IL-4 and transforming growth factor-beta (TGF-β) inhibit nitric oxide synthesis by microglia and astrocytes in culture [9].…”

Section: Introductionmentioning

confidence: 99%

Aqueous humor cytokine levels are associated with the severity of visual field defects in patients with primary open-angle glaucoma

et al. 2023

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Background To evaluate the aqueous humor (AH) levels of cytokines in primary open-angle glaucoma (POAG) patients and cataract patients. Methods Thirty-eight POAG patients and 26 cataract patients were recruited. Peripheral blood (PB) was collected from each subject. The POAG group was divided into 2 subgroups according to the severity of visual field defects. The cutoff point of the mean deviation (MD) of the visual field was -12 dB. AH was obtained at the time of anterior chamber puncture during cataract or glaucoma surgery by using a 27-gauge needle attached to a microsyringe. AH and PB levels of interleukin-2 (IL-2), tumor necrosis factor-alpha (TNF-α), transforming growth factor-beta2 (TGF-β2) and IL-4 were assayed by enzyme-linked immunosorbent assay. Postoperative intraocular pressures (IOPs) of POAG patients were recorded during the follow-up period. Results TNF-α and TGF-β2 showed significantly higher AH levels in the POAG group than in the cataract group (P < 0.001 and P = 0.001, respectively). For the POAG group, preoperative IOPs were significantly positively correlated with AH levels of TNF-α (r2 = 0.129, P = 0.027) and TGF-β2 (r2 = 0.273, P = 0.001). AH levels of TGF-β2 were significantly different among cataract patients, POAG patients with MD> -12 dB and POAG patients with MD≤ -12 dB (P = 0.001). AH levels of TNF-α were significantly positively associated with IOP reduction after trabeculectomy (P = 0.025). AH and PB levels of cytokines were not related to the long-term success of trabeculectomy. Conclusion The levels of TNF-α and TGF-β2 showed different profiles in POAG patients and cataract patients. AH levels of TGF-β2 were correlated with the severity of glaucomatous neuropathy in POAG patients. The findings suggest possible roles for cytokines in the pathogenesis and development of POAG.

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Increased Retinal Ganglion Cell Survival by Exogenous IL-2 Depends on IL-10, Dopamine D1 Receptors, and Classical IL-2/IL-2R Signaling Pathways

Cited by 7 publications

References 82 publications

Ouabain Effects on Human Anaplastic Thyroid Carcinoma 8505C Cells

Ouabain Effects on Human Anaplastic Thyroid Carcinoma 8505C Cells

Multiple Roles of cAMP in Vertebrate Retina

Aqueous humor cytokine levels are associated with the severity of visual field defects in patients with primary open-angle glaucoma

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