Increased RNA production in Saccharomyces cerevisiae by simultaneously overexpressing FHL1, IFH1, and SSF2 and deleting HRP1

Guo, Xuewu; Zhao, Bin; Zhou, Xinran; Ni, Xiaofeng; Lu, Dongxia; Chen, Tingli; Chen, Yefu; Xiao, Dongguang

doi:10.1007/s00253-020-10784-9

Cited by 6 publications

(1 citation statement)

References 32 publications

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“…15 Guo et al increased RNA content in S. cerevisiae by 38.8% through overexpression of FHL1, IFH1, and SSF2 (transcriptional regulators of RP genes and a 60S ribosomal subunit assembly factor, respectively), while simultaneously disrupting the suppressor of cell growth, HRP1. 16 Deletion of GSP2, which is involved in GTP binding and metabolism, and CTT1, an efficient scavenger of H 2 O 2 , or overexpression of the hexose transporter, HXT1, could improve RNA content in S. cerevisiae. 17 However, the specific mechanisms leading to a higher RNA content remain unknown.…”

Section: ■ Introductionmentioning

confidence: 99%

Promoting Nucleic Acid Synthesis in Saccharomyces cerevisiae through Enhanced Expression of Rrn7p, Rrn11p, IMPDH, and Pho84p

Wang,

Xia,

et al. 2023

J. Agric. Food Chem.

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Saccharomyces cerevisiae has emerged as a preferred source for industrial production of ribonucleic acids (RNAs) and their derivatives, which find wide applications in the food and pharmaceutical sectors. In this study, we employed a modified RNA polymerase I-mediated green fluorescent protein expression system, previously developed by our team, to screen and identify an industrial S. cerevisiae strain with an impressive 18.2% increase in the RNA content. Transcriptome analysis revealed heightened activity of genes and pathways associated with rRNA transcription, purine metabolism, and phosphate transport in the high nucleic acid content mutant strains. Our findings highlighted the crucial role of the transcription factor Sfp1p in enhancing the expression of two key components of the transcription initiation factor complex, Rrn7p and Rrn11p, thereby promoting rRNA synthesis. Moreover, elevated expression of 5′-inosine monophosphate dehydrogenases, regardless of the specific isoform (IMD2, 3, or 4), resulted in increased rRNA synthesis through heightened GTP levels. Additionally, exogenous phosphate application, coupled with overexpression of the phosphate transporter PHO84, led to a 61.4% boost in the RNA yield, reaching 2050.4 mg/L. This comprehensive study provides valuable insights into the mechanism of RNA synthesis and serves as a reference for augmenting RNA production in the food industry.

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Section: ■ Introductionmentioning

confidence: 99%