Increased SAMHD1 transcript expression correlates with interferon-related genes in HIV-1-infected patients

Statzu, Maura; Santinelli, Letizia; Viscido, Agnese; Pinacchio, Claudia; Ceccarelli, Giancarlo; Rotondo, Claudia; Scheri, Giuseppe Corano; Mezzaroma, Ivano; Turriziani, Ombretta; Antonelli, Guido; d’Ettorre, Gabriella; Scagnolari, Carolina

doi:10.1007/s00430-018-0574-x

Cited by 7 publications

(2 citation statements)

References 69 publications

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“…Relative quantification of IFN α , IFNβ and housekeeping gene β-glucuronidase target mRNA expression levels were carried out with LightCycler 480 instrument (Roche, Basel, Switzerland), as previously described. [ 13 ]…”

Section: Methodsmentioning

confidence: 99%

Analysis of type I IFN response and T cell activation in severe COVID-19/HIV-1 coinfection

et al. 2020

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Rationale: Complex immune dysregulation in interferon (IFN) and T cell response has been observed in human immunodeficiency virus (HIV-1)-infected patients as well as in coronavirus disease-2019 (COVID-19) patients. However, severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)/HIV-1 coinfection has been described in only few cases worldwide and no data are available on immunological outcomes in HIV-1-patients infected with SARS-CoV-2. Hence, this study aims to compare type I IFN response and T cell activation levels between a SARS-CoV-2/HIV-1-coinfected female patient and age-matched HIV-1-positive or uninfected women. Patient concerns: A 52-year-old woman diagnosed with SARS-CoV-2/HIV-1 coinfection, ten HIV-1-positive women and five age-matched-healthy individuals were enrolled in this study. Diagnoses: SARS-CoV-2 infection caused severe pneumonia in the second week of illness in HIV-1-positive patient under protease inhibitors. Chest high-resolution computed tomography images of the SARS-CoV-2/HIV-1-coinfected patient showed bilateral ground-glass opacities. Interventions: SARS-CoV-2/HIV-1-coinfected female patient under darunavir/cobicistat regimen received a 7-days hydroxychloroquine therapy. Analysis of IFN α /β mRNA levels and CD4 and CD8 T cell (CD38, human leukocyte antigen-DR [HLA-DR], CD38 HLA-DR) frequencies were performed by RT/real-time PCR assays and flow cytometry, respectively. Median relative difference (MRD) was calculated for each immunological variable. For values greater than reference, MRD should be a positive number and for values that are smaller, MRD should be negative. Outcomes: The severe pneumonia observed in SARS-CoV-2/HIV-1-positive patient under protease inhibitors was reversed by a 7-days hydroxychloroquine therapy. At the end of treatment, on day 7, patient reported resolution of fever, normalization of respiratory rate (14 breaths/min), and improved oxygen arterial pressure with a F i O 2 of 30%. MRD values for IFN α /β and CD4 and CD8 T cells expressing CD38 and/or HLA-DR found in SARS-CoV-2-/HIV-1-coinfected woman were approximatively equal to 0 when refereed respectively to HIV-1-positive female patients [MRDs IFN α/ β: median −0.2545 (range: −0.5/0.1); T cells: median −0.11 (range: −0.8/1.3)] and ≥ 6 when referred to healthy individuals [MRDs IFN α/ β: median 28.45 (range: 15/41.9); T cells: median 10 (range 6/22)]. Lessons: These results indicate that SARS-CoV-2 infection in HIV-1-positive female patient was associated with increased levels of IFNα/β-mRNAs and T cell activation compared to healthy individuals.

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Section: Methodsmentioning

confidence: 99%

Analysis of type I IFN response and T cell activation in severe COVID-19/HIV-1 coinfection

et al. 2020

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“…Some cytosolic sensors involved in innate immunity may act in the initial prevention of an autoimmune response [ 10 , 11 ]. In infections, the activity of these sensors may contribute to the restriction or permissiveness of retroviral infections [ 12 , 13 , 14 ]. The three-prime repair exonuclease 1 (TREX1) protein is a sensor that promotes the degradation of DNA dispersed in the cytoplasm (ssDNA and dsDNA), both of endogenous and exogenous origin, contributing to the prevention of autoimmune diseases [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

The Expression Levels of TREX1 and IFN-α Are Associated with Immune Reconstitution in HIV-1-Infected Individuals

Queiroz,

Oliveira,

Moura

et al. 2024

Viruses

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TREX1 acts in the initial prevention of an autoimmune response, but it may contribute to the permissiveness of retrovirus infections. This study investigated the association between the levels of TREX1 gene expression with the polymorphisms TREX1 rs3135941 (T/C) and TREX1 rs3135945 (G/A), and the presence of antinuclear antibodies (ANA) in antiretroviral therapy (ART)-naïve individuals and after 1 year of treatment. Blood samples from 119 individuals with HIV-1 were subjected to genotyping of polymorphisms and quantification of TREX1 gene expression and HIV-1 viral load by qPCR. The concentration of IFN-α and the number of CD4+/CD8+ T lymphocytes were determined by ELISA and flow cytometry, respectively; ANA was investigated by immunofluorescence. A control group of 167 seronegative individuals was used for the comparison of genotypic frequencies. The frequency of the polymorphisms were not associated with HIV infection or with variations in the expression of TREX1 and IFN-α (p > 0.05). ART-naïve individuals exhibited higher TREX1 expression and lower IFN-α expression. After 1 year of ART, TREX1 levels were reduced, while IFN-α and CD4+ T lymphocytes were elevated (p < 0.05). Some individuals on ART presented ANA. These results suggest that ART-mediated restoration of immune competence is associated with a reduction in TREX1 expression, which may induce the development of ANA, regardless of the polymorphism investigated.

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MicroRNAs and the immune system

2022

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Increased SAMHD1 transcript expression correlates with interferon-related genes in HIV-1-infected patients

Cited by 7 publications

References 69 publications

Analysis of type I IFN response and T cell activation in severe COVID-19/HIV-1 coinfection

Analysis of type I IFN response and T cell activation in severe COVID-19/HIV-1 coinfection

The Expression Levels of TREX1 and IFN-α Are Associated with Immune Reconstitution in HIV-1-Infected Individuals

MicroRNAs and the immune system

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