Increased saturated triacylglycerol levels in plasma membranes of human neutrophils stimulated by lipopolysaccharide

May, George L.; Wright, Lesley C.; Obbink, K G; Byleveld, Paul M.; Garg, ML; Ahmad, Zia; Sorrell, Tania C.

doi:10.1016/s0022-2275(20)37174-1

Cited by 15 publications

(6 citation statements)

References 28 publications

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“…S2E). Though TG is a predominant resident of lipid droplets and ER, they can also accumulate in plasma membranes (2-8 mol %) and the above findings support the nanomechanical investigation of cell membrane stiffness in a cell-line dependent fashion [40, 44]. Fourth, a softer cell membrane in PANC-1 could also stem from an increased abundance of medium chain length PC lipids, because (PC constitutes the maximum % (> 40 %) in plasma membranes (SM and PS <10-15), as the long chain lipids would enhance tighter packing of the membrane.…”

Section: Resultssupporting

confidence: 62%

Lipid remodeling by hypoxia aggravates migratory potential in pancreatic cancer while maintaining membrane homeostasis

Agarwala

Nie

Reid

et al. 2022

Preprint

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Membranes are crucial cell components underlying optimal cellular functioning under diverse conditions including cancer. The membrane physiology requires acute maintenance of biophysical properties and a regulation of cellular lipidome. Homeostatic adaptation of membranes to temperature, pressure and anti-cancer drugs is a well-recognized. However, how the same is regulated under the influence of oxygen deprivation in pancreatic cancers-highly hypoxic cancer- is not known. Here, we report robust lipidomic remodelling in response to HIF-1α induction in pancreatic cancer cells and significant accumulation of lipid droplets. The lipidome rewiring span changes across various lipid classes, levels of unsaturation and acyl chain lengths. Interestingly, despite extensive lipidome alteration, cellular membrane homeostatic response ensures no major modulation of membrane biophysical properties underlying enhanced migratory potential. The correlation of lipidome changes, with pathway analysis and proteomics provide the basis for mutually exclusive regulation of lipidome and membrane properties. These findings help to understand the hypoxic regulation of pancreatic membrane homeostasis.

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Section: Resultssupporting

confidence: 62%

Lipid remodeling by hypoxia aggravates migratory potential in pancreatic cancer while maintaining membrane homeostasis

Agarwala

Nie

Reid

et al. 2022

Preprint

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“…59 In keeping with this, LPS-stimulated neutrophils have elevated levels of TG with increased amounts of palmitic acid. 60 A recent study by our group supported these findings of increased fatty acid transport into neutrophils and high levels of palmitic acid entering into beta-oxidation. 8 Alterations in lipid content have been linked to the regulation of human neutrophil function and survival.…”

Section: Lipid Me Tabolis M: Cellul Ar Energ E Ti C S To S I G Naling...supporting

confidence: 57%

“…These triglycerides are thought to act as a source of not just lipid mediators, but also fatty acid for phosphatidylcholine synthesis during phagocytosis 59 . In keeping with this, LPS‐stimulated neutrophils have elevated levels of TG with increased amounts of palmitic acid 60 . A recent study by our group supported these findings of increased fatty acid transport into neutrophils and high levels of palmitic acid entering into beta‐oxidation 8 …”

Section: Lipid Metabolism: Cellular Energetics To Signaling Moleculesmentioning

confidence: 77%

“…Both cholesterol depletion and sphingomyelin degradation result in increased NET formation 61 . The membranes of LPS‐stimulated neutrophils have been shown to display increased fluidity measured by the cholesterol: phospholipid ratio 60 . Lipid rafts have important biological functions related to membrane signaling and protein trafficking.…”

Section: Lipid Metabolism: Cellular Energetics To Signaling Moleculesmentioning

confidence: 99%

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The emerging role for metabolism in fueling neutrophilic inflammation

Morrison

Watts

Sadiku

et al. 2022

Immunological Reviews

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Neutrophils are a critical element of host defense and are rapidly recruited to inflammatory sites. Such sites are frequently limited in oxygen and/or nutrient availability, presenting a metabolic challenge for infiltrating cells. Long believed to be uniquely dependent on glycolysis, it is now clear that neutrophils possess far greater metabolic plasticity than previously thought, with the capacity to generate energy stores and utilize extracellular proteins to fuel central carbon metabolism and biosynthetic activity. Out-with cellular energetics, metabolic programs have also been implicated in the production of neutrophils and their progenitors in the bone marrow compartment, activation of neutrophil antimicrobial responses, inflammatory and cell survival signaling cascades, and training of the innate immune response. Thus, understanding the mechanisms by which metabolic processes sustain changes in neutrophil effector functions and how these are subverted in disease states provides exciting new avenues for the treatment of dysfunctional neutrophilic inflammation which are lacking in clinical practice to date.

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“…These resonances, which are often called MLs, arise mainly from methyl and methylene groups of fatty acyl chains of the neutral lipids triglyceride and cholesteryl ester ( − ). The intensity of the CH 2 resonance is strongly dependent upon the degree of saturation of the fatty acid chains of lipids: the higher is its intensity, the higher is the degree of saturation of the fatty acyl chains of triglyceride and cholesteryl ester ( − ). On the other hand, the intensity of CH 3 depends on the amount of acyl chains and, consequently, on the quantity of triglyceride and cholesteryl ester.…”

Section: Discussionmentioning

confidence: 99%

Environmental Fine Particulate Matter (PM 2.5) Activates the RAW 264.7 Macrophage Cell Line Even at Very Low Concentrations as Revealed by¹H NMR

Santini

Rainaldi

Ferrante

et al. 2003

Chem. Res. Toxicol.

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Because of the association between inhalation of airborne particulate matter (PM) and human respiratory and cardiovascular disease, it is necessary to understand the tissue damage induced by these particles. One of the cell types principally involved in the body's reaction to PM are macrophages, which remove particles in the airway passages and the lungs through phagocytosis. In fact, when macrophages are exposed to a toxic agent such as PM, they undergo a series of changes (including variations in morphology, an increase in glycolysis, and consequent lactate production and the release of cytokines such as interleukin-6 and tumor necrosis factor-alpha) necessary to transform them from "resting" to "activated" macrophages. Because (1)H NMR is extremely useful in monitoring, noninvasively, macrophage metabolism and because this technique has never been utilized to examine macrophage activation after exposure to PM, it was the purpose of the present study to investigate the effects of PM exposure on the RAW 264.7 stabilized macrophage cell line using (1)H NMR spectroscopy. PM with a diameter <2.5 microm (PM 2.5) was utilized because a closer association to mortality and adverse respiratory health effects has been found with this fraction than with particles of a larger size. Measurements were conducted on whole cells at both 500 and 700 MHz as well as on perchloric acid extracts at 700 MHz. Significant variations in numerous metabolites were seen at very low concentrations of PM 2.5. Many of these changes point to activation of RAW 264.7 macrophages even at doses of PM 2.5 much lower than those commonly employed in cell studies. These results are particularly significant since the same concentrations of PM did not induce changes in morphology and release of cytokines in these cells. Therefore, (1)H NMR spectroscopy is an extremely sensitive probe in observing subtle variations in macrophages after exposure to PM 2.5.

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Increased saturated triacylglycerol levels in plasma membranes of human neutrophils stimulated by lipopolysaccharide

Cited by 15 publications

References 28 publications

Lipid remodeling by hypoxia aggravates migratory potential in pancreatic cancer while maintaining membrane homeostasis

Lipid remodeling by hypoxia aggravates migratory potential in pancreatic cancer while maintaining membrane homeostasis

The emerging role for metabolism in fueling neutrophilic inflammation

Environmental Fine Particulate Matter (PM 2.5) Activates the RAW 264.7 Macrophage Cell Line Even at Very Low Concentrations as Revealed by¹H NMR

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Increased saturated triacylglycerol levels in plasma membranes of human neutrophils stimulated by lipopolysaccharide

Cited by 15 publications

References 28 publications

Lipid remodeling by hypoxia aggravates migratory potential in pancreatic cancer while maintaining membrane homeostasis

Lipid remodeling by hypoxia aggravates migratory potential in pancreatic cancer while maintaining membrane homeostasis

The emerging role for metabolism in fueling neutrophilic inflammation

Environmental Fine Particulate Matter (PM 2.5) Activates the RAW 264.7 Macrophage Cell Line Even at Very Low Concentrations as Revealed by1H NMR

Contact Info

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Environmental Fine Particulate Matter (PM 2.5) Activates the RAW 264.7 Macrophage Cell Line Even at Very Low Concentrations as Revealed by¹H NMR