Increased sensitivity of enterotoxigenic Escherichia coli detection in stool samples using oligonucleotide immobilized-magnetic nanoparticles

Jangpatarapongsa, Kulachart; Saimuang, Kween; Polpanich, Duangporn; Thiramanas, Raweewan; Techakasikornpanich, Mongkol; Yudech, Papichaya; Paripurana, Venusrin; Leepiyasakulchai, Chaniya; Tangboriboonrat, Pramuan

doi:10.1016/j.btre.2021.e00677

Cited by 5 publications

(2 citation statements)

References 45 publications

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“…Te DNA extraction of isolates was carried out by the boiling method [19]. To extract the DNA, samples were incubated at 99 °C for 15 min, with adding 0.5% Tween 20, and immediately cooled on ice.…”

Section: Te Pcrmentioning

confidence: 99%

The Detection of Fosfomycin-Modifying Enzymes (fos) in Uropathogenic Enterobacterale, Azerbaijan, Iran

Lalezadeh,

Ghotaslou,

Ghotaslou

2023

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Enterobacteriaceae is the most common agent of urinary tract infections (UTIs). Multidrug resistant (MDR) and XDR (extensively drug-resistant) Enterobacteriaceae in UTIs have increased in the world. The present study aimed to study the fosfomycin resistance frequency and the fosfomycin resistance genes among Enterobacteriaceae isolated from UTIs. The urine was collected and cultured in the standard protocol. To determine the susceptibility testing to fosfomycin in 211 isolates, agar dilution and disk agar diffusion methods were used. MDR was nonsusceptibility to at least one agent in three or more antimicrobial categories. The fosfomycin resistance genes were also evaluated by PCR. The frequency of resistance to fosfomycin was in 14 (6.6%) and 15 (7.1%) isolates by the disk agar diffusion and MIC assays, respectively. However, the MIC50 and MIC90 existed at 8 μg/mL and 16 μg/mL, respectively. The MDR was found in 80%. The frequencies of fosfomycin resistance genes were 5 (33.3%), 3 (20%), 2 (13.3%), 1 (6.6%), and 1 (6.6%) for fosC, fosX, fosA3, fosA, and fosB2, respectively. The fosB and fosC2 were not found. A low resistance rate to fosfomycin is observed. Fosfomycin is still one of the most effective and valuable alternative antibiotics against MDR Enterobacteriaceae isolated from UTIs in our region.

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Section: Te Pcrmentioning

confidence: 99%

The Detection of Fosfomycin-Modifying Enzymes (fos) in Uropathogenic Enterobacterale, Azerbaijan, Iran

Lalezadeh,

Ghotaslou,

Ghotaslou

2023

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Recent studies bring light to an extensive application of nanoparticles in the health area, mainly to detect pathogenic microorganisms (viruses, bacteria, and fungi) or molecules (proteins and genetic material) using biosensors (Barroso et al, 2018;Jangpatarapongsa et al, 2021;Jijie et al, 2018;Kalita et al, 2017;Lopreside et al, 2019). Biosensors are devices for the chemical detection of biological structures (enzymes or cells), which are coupled with an electrochemical transducer and a detector that makes it possible to reveal the target material without the need for the application of substances (Cinti et al, 2017;Basso et al, 2018;Ganganboina et al, 2020).…”

Section: Nanoparticles For Biosensor Constructions In the Diagnosis And Detection Of Infectious And Noninfectious Diseasesmentioning

confidence: 99%

Nanotechnology on agent identification, diseases diagnosis and therapeutic approaches

Reis¹,

Thaler²,

Lustosa³

et al. 2022

biori

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Quantum dot-labeled phage-encoded RBP 55 as a fluorescent nanoprobe for sensitive and specific detection of Salmonella in food matrices

et al. 2023

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Increased sensitivity of enterotoxigenic Escherichia coli detection in stool samples using oligonucleotide immobilized-magnetic nanoparticles

Cited by 5 publications

References 45 publications

The Detection of Fosfomycin-Modifying Enzymes (fos) in Uropathogenic Enterobacterale, Azerbaijan, Iran

The Detection of Fosfomycin-Modifying Enzymes (fos) in Uropathogenic Enterobacterale, Azerbaijan, Iran

Nanotechnology on agent identification, diseases diagnosis and therapeutic approaches

Quantum dot-labeled phage-encoded RBP 55 as a fluorescent nanoprobe for sensitive and specific detection of Salmonella in food matrices

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