Increased Serum Level of 8-Hydroxy-2’-Deoxyguanosine may be an Indication of Pesticides Induced DNA Damage among Indian Farmers

Abstract

The aim of the present hospital based study is to assess the serum levels of 8-hydroxy-2’-deoxyguanosine (8-OHdG) among 152 subjects. A total of 152 subjects, categorized into 3 groups: (i) pesticides exposed group (N=60), (ii) pesticide un-exposed group (N=42) and (iii) healthy controls group (N=50) were recruited for the study following the inclusion and exclusion criteria. The blood was drawn from the eligible subjects and Enzyme Immuno Assay (EIA) was performed to assess the 8-OHdG levels in serum. Appropriate statistical methods were used to analyse the study data. Assessment showed that pesticides exposed group has higher levels of serum 8-OHdG as compared to un-exposed group and healthy controls. Age and duration of exposure had an impact on the levels of serum 8-OHdG.The higher serum levels of 8-OHdG may be a marker for pesticides-induced oxidative DNA damage. Key words: pesticides; exposure; oxidative stress; 8-hydroxy-2’-deoxyguanosine (8-OHdG).

Penggunaan Alat Pelindung Diri Berpengaruh terhadap Kerusakan DNA pada Petani Penyemprot Pestisida di Kecamatan Wuluhan, Kabupaten Jember

“…Adanya senyawa ROS dalam sel, menyebabkan oksidasi pada DNA yang dapat bersifat mutagenik. Senyawa 8-OHdG merupakan hasil pemotongan basa nukleotida akibat proses oksidatif dapat menjadi penanda adanya kerusakan pada DNA petani yang terpapar pestisida (Ledda et al, 2021;Pandiyan et al, 2021). Tingginya kadar 8-OHdG yang terekskresikan dalam urin menandakan tingginya proses oksidasi DNA.…”