Increased serum renalase in hemodialysis patients: is it related to left ventricular hypertrophy?

Oğuz, Ebru Gök; Gürsoy, Güner; Yayar, Özlem; Yıldırım, Tolga; Çi̇men, Tolga; Bulut, Cengiz; Eser, Barış; Canbakan, Başol; Yeter, Ekrem; Aylı, Mehmet Deniz

doi:10.1080/0886022x.2016.1208516

Cited by 12 publications

(10 citation statements)

References 31 publications

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“…Consistent results were obtained by other researchers for patients undergoing both hemodialysis [20–23] and peritoneal dialysis [24]. In all those studies RNLS level was determined by commercially available ELISA kit (USCN Life Science Inc., Wuhan, China) with monoclonal antibodies specific to hRenalase1 isoform.…”

Section: Discussionsupporting

confidence: 79%

Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls

Steć

2017

BMC Nephrol

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BackgroundHuman renalase (RNLS), a recently identified flavoprotein with oxidoreductase activity, is secreted into blood by kidneys and metabolizes circulating catecholamines. Recent studies have revealed that common polymorphisms in RNLS gene might affect the risk of several cardiovascular conditions in hemodialyzed patients. However, the exact mechanism underlying this link remains unclear. The study aims to investigate the association between RNLS gene polymorphisms and plasma renalase level in ESKD patients undergoing hemodialysis (HD group) and healthy controls (HC).MethodsA total of 309 hemodialyzed patients and 90 controls were enrolled in the study. All the participants were genotyped for two RNLS SNPs (rs2576178 and rs10887800) using PCR-RFLP method. Plasma renalase concentrations were determined by enzyme-linked immunosorbent assay (USCN Life Science Inc., Wuhan, China). The IBM SPSS Statistics for Windows, version 20 (IBM Corp., Armonk, NY, USA) was used for statistical analyses.ResultsGenotype distribution and allele frequencies of studied SNPs did not differ between two analyzed groups, p > .050. RNLS concentration in HD group (33.54 μg/mL) was significantly higher than in HC (13.16 μg/mL), p < .001. HD patients with rs10887800AA genotype had lower renalase level (29.32 μg/mL) compared to those with AG (34.52 μg/mL), p < .010 and GG genotype (35.91 μg/mL), p < .010. No significant differences in plasma RNLS between rs10887800AG and GG carriers were observed, p > .050. Interestingly, in HC group rs10887800 polymorphism did not influence RNLS concentration. Rs2576178 SNP did not affect the level of plasma RNLS either in HD group or in HC.ConclusionRs10887800 polymorphic variant of RNLS gene influences the level of circulating RNLS in patients undergoing hemodialysis, and thus elucidates the potentially functional relevance of this polymorphism in HD population.

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Section: Discussionsupporting

confidence: 79%

Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls

Steć

2017

BMC Nephrol

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“…Similar findings were obtained in other studies on patients undergoing both hemodialysis [13] and peritoneal dialysis [14]. Furthermore, no association between plasma RNLS level and left ventricular hyperthrophy (LVH), a cardiac consequence of inadequate HY control, was observed [15]. Oppositely, Dziedzic et al demonstrated a positive correlation between plasma RNLS and mean arterial pressure (MAP) in HD cohort [16].…”

Section: Disscusionsupporting

confidence: 82%

Effect of renalase (RNLS) gene polymorphisms (rs1088700 and rs2576178) on plasma RNLS level in hemodialyzed patients affected by arterial hypertension and coronary artery disease

Steć

2017

Polish Journal of Public Health

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Introduction.We have previously reported that rs10887800 and rs2576178 renalase (RNLS) single nucleotide polymorphisms (SNPs) are associated with the susceptibility to arterial hypertension (HY) and coronary artery disease (CAD) in hemodialyzed patients (HD). However, the underlying mechanism of this link remains undefined.Aim. In the present study we examine the influence of above-mentioned RNLS gene variants on plasma renalase level in subgroups of HD patients affected by HY and CAD.Material and methods. In total, 309 hemodialyzed patients participated in the study (157 males and 152 females, mean age 64.1±14.10 years). Rs10887800 and rs2576178 RNLS gene polymorphisms were genotyped using PCR-RFLP method. Plasma RNLS level was assessed by ELISA (USCN Life Science Inc., Wuhan, China). The data were analyzed using SPSS Statistics 23.Results. Regarding rs10887800 polymorphism, hypertensive AA homozygotes had significantly lower plasma RNLS level (28.93±9.94 µg/mL) compared to AG (34.06±12.79 µg/mL) and GG carriers (36.54±12.01 µg/mL), p=0.002. Among CAD patients no differences in plasma RNLS concentrations between rs10887800AA, AG and GG carriers were observed (31.52±10.95 µg/mL, 34.75±13.37 µg/mL, 34.44±13.10 µg/mL, respectively), p=0.615. For the rs2576178 variant, both HY and CAD participants did not differ in terms of plasma RNLS levels with regard to the particular genotypes, p>0.050.Conclusion. Obtained results extend our previous findings and indicate for the first time that rs10887800 RNLS gene variant modifies the level of plasma RNLS in hemodialyzed patients with HY but not in those with CAD. The study provides, thus, a new insight into the potential mechanisms through which RNLS gene variants modulate the risk of cardiovascular diseases among patients with end-stage kidney disease.

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“…Similar, but a bit lower levels were described by Oguz EG et al (2016); It was shown, that in hemodialysis patients serum renalase levels are significantly higher than in control group (212 ± 127 ng/ml vs. 116 ± 67 ng/ml, respectively; p<0.001) [29]. There is also a report on plasma levels of renalase in hemodialysed men, in which mean renalase levels were just 20.59 ± 32.63 ng/ml, which deviates significantly from the mentioned above results [30].…”

Section: Discussionsupporting

confidence: 66%

Searching for Perfect Marker - Differences and Dependencies in Serum Levels of Renalase, KIM-1, MCP-1, Calbindin and GST-Pi in Patients with Diabetes, Glomerulonephritis and Congenital Defects of the Kidney

Serwin¹,

Wiśniewska²,

Skwirczyńska³

et al. 2018

Preprint

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Diagnosis of kidney diseases has recently become more comprehensive and accurate by using new renal markers. Despite the fact that creatinine and cystatin c have been sufficient in determining kidney function, they did not indicate the exact site of the damage and they were often insufficient in predicting the course of the disease. Aim of the study was to evaluate the potential correlations and differences in levels of six  factors related to kidney function and injury: kidney injury molecule-1 (KIM-1), ncalbindin (CALB), glutathione S-transferase Pi (GST-Pi), calbindin and monocyte chemoattractant protein-1 (MCP-1), between renal patients with diabetic nephropathy (DM), congenital defects (CD) of the kidney and glomerulonephritis (GN). Study involved 75 patients: 49 with diabetic nephropathy, 12 with congenital defects and 14 with glomerulonephritis. Levels of renalase was measured using immunoenzymatic tests. Levels of other markers: calbindin, glutathione-S-transferase (GST-pi), interleukin-18 (IL-18), kidney injury molecule-1 (KIM-1) and monocyte chemoattractant protein-1 (MCP-1), were analyzed using Kidney Toxicity-1 Panel and BioPlex system, designed for analyses in urine and optimized by us for serum.From all analyzed markers, only levels of KIM-1 differed significantly between any subgroups, and that was for CD and DM. Renalase correlated significantly negatively with creatinine and positively with all other markers, apart from MCP-1. Obtained results indicate, that serum renalase, KIM-1, calbindin and GST-pi are related to kidney function, with KIM-1 being the most exact, while MCP-1 levels are unrelated to creatinine and glucose levels, does not differ between patients with diabetic nephropathy and other subgroups, and therefore seem to be independent of diabetes. Also, serum-optimized Kidney Toxicity Panel 1 kit for determination of selected markers gave results similar to previous ones and therefore the method can be valuable in determination of analyzed factors.

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Increased serum renalase in hemodialysis patients: is it related to left ventricular hypertrophy?

Cited by 12 publications

References 31 publications

Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls

Rs10887800 renalase gene polymorphism influences the level of circulating renalase in patients undergoing hemodialysis but not in healthy controls

Effect of renalase (RNLS) gene polymorphisms (rs1088700 and rs2576178) on plasma RNLS level in hemodialyzed patients affected by arterial hypertension and coronary artery disease

Searching for Perfect Marker - Differences and Dependencies in Serum Levels of Renalase, KIM-1, MCP-1, Calbindin and GST-Pi in Patients with Diabetes, Glomerulonephritis and Congenital Defects of the Kidney

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