Increased sister chromatid exchange frequency in young women with breast cancer and in their first-degree relatives

Çefle, Kıvanç; Ucur, Ali; Güney, Neşe; Öztürk, Şükrü; Palandüz, Şükrü; Taş, Faruk; Asoğlu, Oktar; Bayrak, Ayşegül; Müslümanoğlu, Mahmut; Aydıner, Adnan

doi:10.1016/j.cancergencyto.2006.06.005

Cited by 16 publications

(12 citation statements)

References 24 publications

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“…Similar results were found in breast cancer patients by Cefle et al They found that the SCE level of control group significantly lowers than patients and their relatives. However, the frequencies of SCE were not different between patients and their relatives (15). In contrast to our study, they found a significant difference between the relative group and the control group.…”

Section: Figure 1 Sce Distribution Of Measurements According To Groupscontrasting

confidence: 99%

“…Bromodeoxyuridine (BrdU; Sigma Chemical Company, USA) was added to each flask at the 24th hour of culture, and then lymphocytes incubated for another 48 hours in darkness. At the end of the 72 hours, cell was harvested with standard fluorescence plus Giemsa techniques, and then metaphase figures were obtained (8,15). For each patient, 20 metaphase spreads were assessed with a light microscope (100X).…”

Section: Methodsmentioning

confidence: 99%

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Increased Sister Chromatid Exchanges in Patients with Gastrointestinal Cancers and in their First-Degree Relatives

Turgut

Yaşar²,

Yaykaşlı

et al. 2014

Electron J Gen Med

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Gastrointestinal Cancers (GICs) European Journal of General MedicineOriginal Article Eur J Gen Med 2014; 11(2): 94-98 DOI : 10.15197/sabad.1.11. 46 Eur J Gen Med 2014;11(2): 94-98 Increased sister chromatid exchange in gastrointestinal cancers 95 MATERIALS AND METHODSThis study was conducted at Düzce University Medical Faculty, Department of Medical Genetics, Düzce, Turkey. The study groups consisted of 15 GIC patients (2 esophagus, 6 stomach, 6 colon and 1 rectum), 13 relatives and 15 healthy subjects. Approved ethical certificate was obtained from local ethics committee, and written informed consents of the studied cases were obtained. Age, gender, smoking and alcohol consumption status and tumor markers (alpha-fetoprotein, AFP, carcinoembryonic antigen-CEA, carcinogenic antigen (CA) 125, CA19-9, CA15-3) were recorded. Two milliliters of venous blood was drawn using Na-heparinized syringes from each case. Replication Index was calculated by following formula.RPI: R1 + (R2x2) + (R3x3) / R1 + R2 + R3Mitotic index (MTI) was calculated by following formula. MTI: metaphase/metaphase+lymphocyte count Statistical analysisThe frequency of SCE per metaphase in groups was compared by Kruskal Wallis. Mann-Whitney U test was also used. Relationships between parameters were evaluated with the Spearman's correlation analysis. Qualitative data were compared using Chi-square test. Results with 95% confidence interval and p<0.05 were considered significant.

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Section: Figure 1 Sce Distribution Of Measurements According To Groupscontrasting

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Increased Sister Chromatid Exchanges in Patients with Gastrointestinal Cancers and in their First-Degree Relatives

Turgut

Yaşar²,

Yaykaşlı

et al. 2014

Electron J Gen Med

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“…This result revealed the breast cancer did not remarkably affect the frequencies of SCE (Dhillon et al, 1995). On the other hand, there are some contradictory investigations in which relationship between SCE and breast cancer indicated (Aristei et al, 2009;Cefle et al, 2006;Roy et al, 2000;Adhvaryu et al, 1988). These reports have been interpreted affective confounding factors all together.…”

Section: Discussionmentioning

confidence: 88%

Sister chromatid exchanges in breast cancer patients who underwent chemotherapy

2012

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-The study aimed to determine the effects of cytostatic and genotoxic drugs used to treat breast cancer on sister chromatid exchange (SCE). SCE values were examined in 25 female patients with breast cancer in pre-treatment, treatment process and remission period as well as in 22 nonsmoker women via peripheral blood culture technique. The SCE values of patient and control group were analyzed via "Mann-Whitney U test". Whilst SCE values of patient group were 8.25 ± 3.67, 10.19 ± 2.95 and 11.52 ± 3.33 in pre-treatment, treatment process and remission periods respectively, it was 7.01 ± 1.24 in control group. When overall SCE values of patients group in pre-treatment period were compared with those of control group, no significant difference was observed (p > 0.05), whereas highly significant differences were observed between treatment process and remission period of patient groups and control group in terms of SCE values (p < 0.01). If patients are exposed to any cytostatic and clastogenic drugs, the increase in the exchange values was considered remarkable. These findings reinforced the availability of sister chromatid exchange technique in directing of treatment and monitoring the genetic abnormalities caused by genomic instability which may occur due to the drugs used for treatment.

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“…An increased SCE frequency in patients and their first-degree relatives has also been demonstrated in some previous studies. Cefle et al [22] showed that SCE frequency was significantly higher in young women with breast cancer (n=20, all <40 years old) and in their first-degree relatives (n=20), compared with controls (n=20), all of whom were from western Turkey. They concluded that patients with early-onset breast cancer exhibit increased genomic instability, as do their first-degree relatives.…”

Section: Discussionmentioning

confidence: 99%

Cytogenetic Finding of Breast Cancer Cases and in Their First-Degree Relatives

et al. 2013

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PurposeThe aim of this study was to evaluate and compare the rate of sister chromatid exchange (SCE), the occurrence of micronuclei, and the lymphocyte proliferation rate index (PRI) in patients with breast cancer, their first-degree relatives, and healthy volunteers.MethodsWe analyzed the frequency of SCE and micronuclei, and the PRI in the peripheral blood lymphocytes of 30 women with breast cancer, 22 of their female family members, and 20 age-matched healthy female volunteers.ResultsSCE occurred significantly more often in the lymphocytes of breast cancer patients (10.84±0.4 per metaphase), compared with their first-degree relatives (7.45±0.54) and controls (5.94±0.2) (p<0.001 for both). The mean SCE frequency was not statistically different between first-degree relatives and controls (p=0.071). Similarly, micronuclei occurred at a significantly higher rate in breast cancer patients (9.6±0.72), and in their first-degree relatives (7±0.64), compared to controls (3.85±0.4) (p<0.001 and p=0.001, respectively). There was also a significant difference between the occurrence of micronuclei in patients compared to their family members (p=0.021). The PRI was significantly lower in patients (1.61±0.1), compared with both their first-degree relatives (1.75±0.1), and controls (1.74±0.1) (p=0.001 and p=0.002, respectively).ConclusionIncreased SCE and the occurrence of micronuclei, as well as a reduced PRI are associated with breast cancer. Furthermore, increased SCE and the frequency of micronuclei in a first-degree relative suggest that they exhibit greater genetic instability than women of the same age.

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Increased sister chromatid exchange frequency in young women with breast cancer and in their first-degree relatives

Cited by 16 publications

References 24 publications

Increased Sister Chromatid Exchanges in Patients with Gastrointestinal Cancers and in their First-Degree Relatives

Increased Sister Chromatid Exchanges in Patients with Gastrointestinal Cancers and in their First-Degree Relatives

Sister chromatid exchanges in breast cancer patients who underwent chemotherapy

Cytogenetic Finding of Breast Cancer Cases and in Their First-Degree Relatives

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