Increased Stability of Glycol-Terminated Self-Assembled Monolayers for Long-Term Patterned Cell Culture

Strulson, Matthew K.; Johnson, Dawn M.; Maurer, Joshua A.

doi:10.1021/la2035533

Cited by 23 publications

(33 citation statements)

References 30 publications

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“…After that the individual cell outlines were manually traced and the threshold intensity ranges were set at 65-255 as described earlier. [70,73] Finally, the size parameters of the vinculin plaques were set between 0 and 6 µm 2 and number and area of the plaques were counted by the software. Up to 20 confocal planes were captured along the z-axis in order to perform 3D reconstructions and estimate the cell thickness.…”

Section: Methodsmentioning

confidence: 99%

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Zhukova

Hiepen

Knaus

et al. 2017

Adv Healthcare Materials

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Surface structuring of titanium-based implants is known to modulate the behavior of adherent cells, but the influence of different nanotopographies is poorly understood. The aim is to investigate preosteoblast proliferation, adhesion, morphology, and migration on surfaces with similar surface chemistry but distinct nanotopographical features. Sonochemical treatment and anodic oxidation are employed to fabricate disordered, mesoporous titania (TMS) and ordered titania nanotubular (TNT) topographies on titanium, respectively. Morphological evaluation reveals that cells are polygonal and well-spread on TMS, but display an elongated, fibroblast-like morphology on TNT surfaces, while they are much flatter on glass. Both nanostructured surfaces impair cell adhesion, but TMS is more favorable for cell growth due to its support of cell attachment and spreading in contrast to TNT. A quantitative wound healing assay in combination with live-cell imaging reveals that cell migration on TMS surfaces has a more collective character than on other surfaces, probably due to a closer proximity between neighboring migrating cells on TMS. The results indicate distinctly different cell adhesion and migration on ordered and disordered titania nanotopographies, providing important information that can be used in optimizing titanium-based scaffold design to foster bone tissue growth and repair while allowing for the encapsulation of drugs into porous titania layer

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Section: Methodsmentioning

confidence: 99%

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

Zhukova

Hiepen

Knaus

et al. 2017

Adv Healthcare Materials

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“…The vast majority of surface-anchored initiators involve the formation of a self-assembled monolayer (SAM) on an appropriate substrate [26]. While this strategy has worked well, SAMs have limited long-term stability to a variety of reagents [28][29][30][31]. Furthermore, SAMs are not substrate-independent, as they require a new initiator for every substrate type [26,32].…”

Section: Synthetic Approaches and Challengesmentioning

confidence: 99%

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

et al. 2015

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Abstract:In this review, we describe the latest advances in synthesis, characterization, and applications of polymer brushes. Synthetic advances towards well-defined polymer brushes, which meet criteria such as: (i) Efficient and fast grafting, (ii) Applicability on a wide range of substrates; and (iii) Precise control of surface initiator concentration and hence, chain density are discussed. On the characterization end advances in methods for the determination of relevant physical parameters such as surface initiator concentration and grafting density are discussed. The impact of these advances specifically in emerging fields of nano-and bio-technology where interfacial properties such as surface energies are controlled to create nanopatterned polymer brushes and their implications in mediating with biological systems is discussed.

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“…For example, cells can scrape off microprinted adhesion molecules which often limits the durability of the pattern to several days in some cases [31,32]. In the past, the combination of micro contact printing of cell adhesive molecules such as fibronectin and adsorption of blocking agents such as polyethylene glycol was commonly used for cell patterning [33].…”

Section: Introductionmentioning

confidence: 99%

Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells

Tanaka¹,

Yamashita

Sato³

et al. 2017

PLoS ONE

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The geometrical confinement of small cell colonies gives differential cues to cells sitting at the periphery versus the core. To utilize this effect, for example to create spatially graded differentiation patterns of human mesenchymal stem cells (hMSCs) in vitro or to investigate underpinning mechanisms, the confinement needs to be robust for extended time periods. To create highly repeatable micro-fabricated structures for cellular patterning and high-throughput data mining, we employed here a simple casting method to fabricate more than 800 adhesive patches confined by agarose micro-walls. In addition, a machine learning based image processing software was developed (open code) to detect the differentiation patterns of the population of hMSCs automatically. Utilizing the agarose walls, the circular patterns of hMSCs were successfully maintained throughout 15 days of cell culture. After staining lipid droplets and alkaline phosphatase as the markers of adipogenic and osteogenic differentiation, respectively, the mega-pixels of RGB color images of hMSCs were processed by the software on a laptop PC within several minutes. The image analysis successfully showed that hMSCs sitting on the more central versus peripheral sections of the adhesive circles showed adipogenic versus osteogenic differentiation as reported previously, indicating the compatibility of patterned agarose walls to conventional microcontact printing. In addition, we found a considerable fraction of undifferentiated cells which are preferentially located at the peripheral part of the adhesive circles, even in differentiation-inducing culture media. In this study, we thus successfully demonstrated a simple framework for analyzing the patterned differentiation of hMSCs in confined microenvironments, which has a range of applications in biology, including stem cell biology.

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Increased Stability of Glycol-Terminated Self-Assembled Monolayers for Long-Term Patterned Cell Culture

Cited by 23 publications

References 30 publications

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

The Role of Titanium Surface Nanostructuring on Preosteoblast Morphology, Adhesion, and Migration

From Self-Assembled Monolayers to Coatings: Advances in the Synthesis and Nanobio Applications of Polymer Brushes

Simple agarose micro-confinement array and machine-learning-based classification for analyzing the patterned differentiation of mesenchymal stem cells

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