Increased Stromal Infiltrating Lymphocytes Are Associated with the Risk of Disease Progression in Mesenchymal Circulating Tumor Cell-Positive Primary Breast Cancer Patients

Smolkova, Bozena; Čierna, Zuzana; Kalavska, Katarína; Miklikova, Svetlana; Plavá, Jana; Minárik, Gabriel; Sedláčková, Tatiana; Cholujová, Dana; Gronesova, Paulina; Cihova, Marina; Majerova, Karolina; Karaba, Marián; Benca, Juraj; Pinďák, Daniel; Mardiak, Jozef; Mego, Michal

doi:10.3390/ijms21249460

Cited by 7 publications

(5 citation statements)

References 52 publications

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“…Methods for counting were adapted from published methods used to analyze T cell infiltrates. 27 The mean count of the scores for each subject was recorded and used in analysis.…”

Section: Morphological Evaluation Of Tumor-infiltrating Lymphocytesmentioning

confidence: 99%

Areca nut extract increased stromal tumor-infiltrating lymphocytes in 4-nutriquinoline-1-oxide-tumor-induced Sprague-Dawley rats

Sari,

Novita,

Andriany

et al. 2024

F1000Res

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Background Oral squamous cell carcinoma (OSCC) is one of the most common oral cancers with a high mortality rate. The biodiversity source in Indonesia makes areca nut a potential antioxidant in treating disease. Objective The study aimed to evaluate the effect of areca nut extract in 4-nutriquinoline-1-oxide (NQO)-tumor-induced rats. Methods Twenty-eight male Sprague-Dawley rats were divided into four groups. Group 1 served as the control group, group 2 was 4NQO-induced rats without treatment, and groups 3 and 4 were given 4NQO-tumor inducer with 500 and 1000 mg/kg BW of areca nut extract, respectively. The rats in groups 2,3, and 4 received 30 ppm of 4NQO tumor inducer in drinking water for 12 weeks. In the end, all rats were euthanized and the tongue was removed. The body, liver, kidney, heart, and lungs weights were measured. Tongue tumor volume and dysplasia lesions were analyzed. The tumor-infiltrating lymphocytes (TILs) in the tumor and stromal area were scored semi-quantitatively associating the infiltrate grade (0-3) and analyzed histologically. Results There were significant differences in body weight loss between the initial and final phases in groups 1 and 2 (p<0.05). The areca nut at doses of 500 and 1000 mg/kg BW significantly reduced the tumor size compared with groups 1 and 2 (p<0.05). The incidence of OSCC in rats with 500 and 1000 mg/kg BW of areca nut extract after 22 weeks was 0%, but the dysplasia lesions were observed at 28.57% and 85.71%, respectively. The highest mean of stromal TILs was in group 3 and there were significant differences in stromal TILs between groups 2 and 3 (p<0.05). Conclusion Areca nut extract in 4NQO-induced rats by inducing infiltrating lymphocytes in the stromal tumor area on the OSCC lesion of the tongue.

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“…Methods for counting were adapted from published methods used to analyze T cell infiltrates. 27 The mean count of the scores for each subject was recorded and used in analysis.…”

Section: Morphological Evaluation Of Tumor-infiltrating Lymphocytesmentioning

confidence: 99%

Areca nut extract increased stromal tumor-infiltrating lymphocytes in 4-nutriquinoline-1-oxide-tumor-induced Sprague-Dawley rats

Sari,

Novita,

Andriany

et al. 2024

F1000Res

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“…Epithelial-originated CTCs are distinguishable from other phenotypes due to the expression of EpCAM and cytokeratin [ 90 , 91 , 92 ]. Since mesenchymal CTCs lack cytokeratins and CD45, they are less likely to be isolated from the blood, which also makes them more resistant and more prone to metastasis [ 93 , 94 ]. Apoptotic CTCs result from the process of programmed cell death, the level of which provides a reference for evaluating treatment effects [ 95 , 96 ].…”

Section: Overview Of Biomarkers In Hnscc—cfdna Circulating Tumor Cell...mentioning

confidence: 99%

Extracellular Vesicles as Biomarkers in Head and Neck Squamous Cell Carcinoma: From Diagnosis to Disease-Free Survival

Chen

Leung

et al. 2023

Cancers

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Head and neck squamous cell carcinomas (HNSCCs) arising from different anatomical sites present with different incidences and characteristics, which requires a personalized treatment strategy. Despite the extensive research that has conducted on this malignancy, HNSCC still has a poor overall survival rate. Many attempts have been made to improve the outcomes, but one of the bottlenecks is thought to be the lack of an effective biomarker with high sensitivity and specificity. Extracellular vesicles (EVs) are secreted by various cells and participate in a great number of intercellular communications. Based on liquid biopsy, EV detection in several biofluids, such as blood, saliva, and urine, has been applied to identify the existence and progression of a variety of cancers. In HNSCC, tumor-derived EVs exhibit many functionalities by transporting diverse cargoes, which highlights their importance in tumor screening, the determination of multidisciplinary therapy, prediction of prognosis, and evaluation of therapeutic effects. This review illustrates the classification and formation of EV subtypes, the cargoes conveyed by these vesicles, and their respective functions in HNSCC cancer biology, and discloses their potential as biomarkers during the whole process of tumor diagnosis, treatment, and follow-up.

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“…CTC detection was performed as described in full details previously [37]. Briefly, CTCs were detected in peripheral blood by a reverse transcription quantitative PCR (RT-qPCR) based assay utilising cells after CD45 depletion using the RossetteSep kit (Stem Cell Technologies, Vancouver, BC, Canada).…”

Section: Ctc Status Detectionmentioning

confidence: 99%

Uncovering Microbial Composition in Human Breast Cancer Primary Tumour Tissue Using Transcriptomic RNA-seq

Hadžega

Minárik

Karaba³

et al. 2021

IJMS

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Recent research studies are showing breast tissues as a place where various species of microorganisms can thrive and cannot be considered sterile, as previously thought. We analysed the microbial composition of primary tumour tissue and normal breast tissue and found differences between them and between multiple breast cancer phenotypes. We sequenced the transcriptome of breast tumours and normal tissues (from cancer-free women) of 23 individuals from Slovakia and used bioinformatics tools to uncover differences in the microbial composition of tissues. To analyse our RNA-seq data (rRNA depleted), we used and tested Kraken2 and Metaphlan3 tools. Kraken2 has shown higher reliability for our data. Additionally, we analysed 91 samples obtained from SRA database, originated in China and submitted by Sichuan University. In breast tissue, the most enriched group were Proteobacteria, then Firmicutes and Actinobacteria for both datasets, in Slovak samples also Bacteroides, while in Chinese samples Cyanobacteria were more frequent. We have observed changes in the microbiome between cancerous and healthy tissues and also different phenotypes of diseases, based on the presence of circulating tumour cells and few other markers.

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Increased Stromal Infiltrating Lymphocytes Are Associated with the Risk of Disease Progression in Mesenchymal Circulating Tumor Cell-Positive Primary Breast Cancer Patients

Cited by 7 publications

References 52 publications

Areca nut extract increased stromal tumor-infiltrating lymphocytes in 4-nutriquinoline-1-oxide-tumor-induced Sprague-Dawley rats

Areca nut extract increased stromal tumor-infiltrating lymphocytes in 4-nutriquinoline-1-oxide-tumor-induced Sprague-Dawley rats

Extracellular Vesicles as Biomarkers in Head and Neck Squamous Cell Carcinoma: From Diagnosis to Disease-Free Survival

Uncovering Microbial Composition in Human Breast Cancer Primary Tumour Tissue Using Transcriptomic RNA-seq

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