Increased substrate availability reveals the potential of scentless lisianthus flowers in producing fragrant benzenoid‐phenylpropanoids

Fang, Fang; Oliva, Moran; Ovadia, Rinat; Bar, Einat; Nissim-Levi, Ada; Wang, Ru; Neeman, Agmon; Zaccai, Michèle; Lewinsohn, Efraim; Oren-Shamir, Michal

doi:10.1111/ppl.13264

Cited by 9 publications

(4 citation statements)

References 28 publications

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“…Current studies appear to focus mainly on the identification of floral aroma components, together with their dynamic temporal and spatial changes [15,16]. For example, the biosynthetic pathways for benzyl acetate and eugenol both belong to the broader phenylpropene metabolic pathway, which has been better characterised in the model plants petunia and Arabidopsis [17,18]-with the synthesis of benzyl acetate catalysed by benzyl alcohol acetyltransferase (BEAT), using benzyl alcohol and acetyl CoA as substrates [19].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis linked to key volatiles reveals molecular mechanisms of aroma compound biosynthesis in Prunus mume

Wang

Song

et al. 2022

BMC Plant Biol

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Background Mei (Prunus mume) is the only woody plant in the genus Prunus with a floral fragrance, but the underlying mechanisms of aroma compound biosynthesis are unclear despite being a matter of considerable interest. Results The volatile contents of the petals of two cultivars with significantly different aromas, Prunus mume ‘Xiao Lve’ and Prunus mume ‘Xiangxue Gongfen’, were characterised by GC-MS at different flowering periods, and a total of 44 volatile compounds were detected. Among these, the main substances forming the typical aroma of P. mume were identified as eugenol, cinnamyl acetate, hexyl acetate and benzyl acetate, with variations in their relative concentrations leading to sensory differences in the aroma of the two cultivars. We compiled a transcriptome database at key stages of floral fragrance formation in the two cultivars and used it in combination with differential analysis of floral volatiles to construct a regulatory network for the biosynthesis of key aroma compounds. The results indicated that PmPAL enzymes and PmMYB4 transcription factors play important roles in regulating the accumulation of key biosynthetic precursors to these compounds. Cytochrome P450s and short-chain dehydrogenases/reductases might also influence the biosynthesis of benzyl acetate by regulating production of key precursors such as benzaldehyde and benzyl alcohol. Furthermore, by analogy to genes with verified functions in Arabidopsis, we predicted that three PmCAD genes, two 4CL genes, three CCR genes and two IGS genes all make important contributions to the synthesis of cinnamyl acetate and eugenol in P. mume. This analysis also suggested that the downstream genes PmBGLU18-like, PmUGT71A16 and PmUGT73C6 participate in regulation of the matrix-bound and volatile states of P. mume aroma compounds. Conclusions These findings present potential new anchor points for further exploration of floral aroma compound biosynthesis pathways in P. mume, and provide new insights into aroma induction and regulation mechanisms in woody plants.

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptome analysis linked to key volatiles reveals molecular mechanisms of aroma compound biosynthesis in Prunus mume

Wang

Song

et al. 2022

BMC Plant Biol

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“…Thus, expression of dedicated transcription factors for the activation of multiple genes in a pathway(s) is a routine approach in plant metabolic engineering (Koeduka et al ., 2021; Skaliter et al ., 2019; Zhang et al ., 2015; Zvi et al ., 2008, 2012). In some cases, the genetic components are not actively producing target products because the substrate is missing, a phenomenon known as ‘silent metabolism’ (Fang et al ., 2021; Hoffmann et al ., 2011; Lewinsohn and Gijzen, 2009). For example, lisianthus flowers that are normally unscented produced the Phe‐derived methyl benzoate and 2‐phenylethanol when precursor availability was increased (Fang et al ., 2021).…”

Section: Microbes and Plants As Platforms For Aroma Compound Productionmentioning

confidence: 99%

“…In some cases, the genetic components are not actively producing target products because the substrate is missing, a phenomenon known as ‘silent metabolism’ (Fang et al ., 2021; Hoffmann et al ., 2011; Lewinsohn and Gijzen, 2009). For example, lisianthus flowers that are normally unscented produced the Phe‐derived methyl benzoate and 2‐phenylethanol when precursor availability was increased (Fang et al ., 2021). Another advantage is that the final biosynthetic steps of many specialized metabolites are not fully detailed, and plants offer the possibility to complete these uncharacterized reactions (Dong et al ., 2012; Jacobowitz and Weng, 2020; Zhou et al ., 2018).…”

Section: Microbes and Plants As Platforms For Aroma Compound Productionmentioning

confidence: 99%

A whiff of the future: functions of phenylalanine‐derived aroma compounds and advances in their industrial production

Skaliter

Livneh

Agron

et al. 2022

Plant Biotechnology Journal

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Summary Plants produce myriad aroma compounds—odorous molecules that are key factors in countless aspects of the plant's life cycle, including pollinator attraction and communication within and between plants. For humans, aroma compounds convey accurate information on food type, and are vital for assessing the environment. The phenylpropanoid pathway is the origin of notable aroma compounds, such as raspberry ketone and vanillin. In the last decade, great strides have been made in elucidating this pathway with the identification of numerous aroma‐related biosynthetic enzymes and factors regulating metabolic shunts. These scientific achievements, together with public acknowledgment of aroma compounds' medicinal benefits and growing consumer demand for natural products, are driving the development of novel biological sources for wide‐scale, eco‐friendly, and inexpensive production. Microbes and plants that are readily amenable to metabolic engineering are garnering attention as suitable platforms for achieving this goal. In this review, we discuss the importance of aroma compounds from the perspectives of humans, pollinators and plant–plant interactions. Focusing on vanillin and raspberry ketone, which are of high interest to the industry, we present key knowledge on the biosynthesis and regulation of phenylalanine‐derived aroma compounds, describe advances in the adoption of microbes and plants as platforms for their production, and propose routes for improvement.

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“…Among the most cultivated in Ciência Rural, v.54, n.9, 2024. Barbosa et al the world (FANG et al, 2021), they are native to the arid areas of the Southern United States and Northern Mexico (GÓMEZ-PÉREZ et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Lisianthus (Eustoma grandiflorum) leaf degradation analysis in the postharvest by VIS-NIR-SWIR reflectance spectroscopy

Barbosa,

Fiorio,

Calaboni

et al. 2024

Cienc. Rural

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It is known that floriculture is very important for the world economic scenario. Nevertheless, the vase life of cut flowers is determined by a short time span. This study evaluated the spectral behavior during leaf degradation of lisianthus (Eustoma grandiflorum) flower stems in the postharvest, in different preservative solutions, and estimating its leaf pigments by hyperspectral data. Lisianthus floral stems were subjected to preservative solutions with different concentrations of sucrose, glucose, phytohormones and deionized water. Leaves from these stems were collected every 4 days for further hyperspectral analysis. Spectra was determined in laboratory with a sensor collecting at the range of 350-2500 nm. In order to analyze the use of the spectra to detect the performance of the preservative solutions, statistical tests were used at each wavelength. Pigment prediction was assessed by the CAR/CLF ratio (Ratio between Carotenoids and Chlorophyll) from the spectral curves, using PLSR and RRMSE. The glucose-based solutions registered positive effects on the preservation of floral stems, especially at the dose of 180 g/L. The solutions based on sucrose and phytohormones registered similarities in the spectral curves among the doses, demonstrating equality in leaf preservation. The discriminant analyses demonstrated there was statistical difference in the spectral responses in the doses for each solution used. The CAR/CLF ratio had reasonable coefficients of 0.6 and RRMSE below 6.99%. The hyperspectral analyses presented a potential for the evaluation of leaf degradation in lisianthus floral stems in different pulsing solutions used in the postharvest.

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Increased substrate availability reveals the potential of scentless lisianthus flowers in producing fragrant benzenoid‐phenylpropanoids

Cited by 9 publications

References 28 publications

Comparative transcriptome analysis linked to key volatiles reveals molecular mechanisms of aroma compound biosynthesis in Prunus mume

Comparative transcriptome analysis linked to key volatiles reveals molecular mechanisms of aroma compound biosynthesis in Prunus mume

A whiff of the future: functions of phenylalanine‐derived aroma compounds and advances in their industrial production

Lisianthus (Eustoma grandiflorum) leaf degradation analysis in the postharvest by VIS-NIR-SWIR reflectance spectroscopy

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