Increased Superoxide Generation is Associated with Decreased Superoxide Dismutase Activity and mRNA Expression in Placental Trophoblast Cells in Pre-eclampsia

Wang, Y; Walsh, Scott W.

doi:10.1053/plac.2000.0608

Cited by 210 publications

(111 citation statements)

References 18 publications

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“…High oxidative stress was found to be related to adverse pregnancy outcomes including low birth weight (34,41) , and presumably this inverse association would persist into postnatal growth. Although there was no statistical significance, our study revealed a potential association between high oxidative stress during pregnancy and reduced infant weight and height both at birth and after birth.…”

Section: Discussionmentioning

confidence: 99%

Association of antioxidant vitamins and oxidative stress levels in pregnancy with infant growth during the first year of life

Hong

Park²,

Kim³

et al. 2008

Public Health Nutr.

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Objective: Whereas there are numerous reports in the literature relating the impact of maternal nutritional status on subsequent birth outcome, much less is known about the long-term impact on infant growth after birth. Therefore, we conducted a prospective cohort study to investigate the association of maternal micronutrient status (vitamins A, C and E, folate) and oxidative stress status in pregnancy with infant growth during the first year of life. Design: Prospective cohort study. Setting: Outpatient clinic of obstetrics, Ewha Womans University Hospital, Seoul, South Korea. Subjects and methods: Two groups were constructed for this study -the Ewha pregnancy cohort (n 5 677) and the infant growth cohort comprising follow-up live newborns of all the recruited pregnant women (n 5 317). Maternal serum vitamin and urinary oxidative stress levels were collected and infant weights and heights were measured at birth and at 6 and 12 months after birth. Results: Division of the subjects into folate-deficient and normal groups revealed that infant weight and height at 0, 6 and 12 months were adversely affected by folate deficiency. High maternal vitamin C was associated with increased infant weight and height at birth and after birth. Conclusion: Our findings indicate the importance of preventing folate deficiency and supplementing vitamin C during pregnancy.

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Section: Discussionmentioning

confidence: 99%

Association of antioxidant vitamins and oxidative stress levels in pregnancy with infant growth during the first year of life

Hong

Park²,

Kim³

et al. 2008

Public Health Nutr.

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“…The pancreatic beta cells require large amounts of ATP to allow glucose stimulated insulin secretion to take place. Studies in humans have shown that intrauterine growth retardation can lead to increased production of reactive oxygen species (ROS) in the fetus (71)(72)(73) along with low levels of oxygen which lead to impaired function of the electron transport chain, which can then increase ROS production further (74,75). The oxidative damage M a n u s c r i p t 12 caused by ROS is not limited to the mitochondria -proteins, lipids and nucleic acids within the cell can also be damaged.…”

Section: Mechanisms Of Intrauterine Programmingmentioning

confidence: 99%

Fetal programming of glucose–insulin metabolism

Jones

Ozanne

2009

Molecular and Cellular Endocrinology

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Epidemiological studies have shown a link between poor fetal growth and increased risk of developing type 2 diabetes. These observations are highly reproducible in many populations worldwide although the mechanisms behind them remain elusive.The 'Thrifty Phenotype Hypothesis' was proposed to explain the underlying causes of these relationships. Animal models of poor intrauterine nutrition have been utilised to help to define the causal factors and identify the molecular mechanisms. Programmed changes in beta cell function and insulin action have been a common feature of animal models of poor intrauterine nutrition. Fundamental underlying mechanisms are starting to emerge, including changes in the epigenotype and mitochondrial function.

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“…Placental trophoblasts were isolated as previously described (12). Briefly, placental villous tissues, excluding chorionic and basal plates, were digested by Dispase (BD Biosciences, Bedford, MA).…”

Section: Trophoblast Isolation and Culturementioning

confidence: 99%

Predominant Basal Directional Release of Thromboxane, but not Prostacyclin, by Placental Trophoblasts from Normal and Preeclamptic Pregnancies

Zhao

Lewis

et al. 2008

Placenta

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Objective-To investigate apical and basal releases of thromboxane (TX) and prostacyclin (PGI2) by trophoblasts (TCs) from normal and preeclamptic (PE) placentas.Methods-TCs isolated from normal and PE placentas were incubated in cell culture inserts for 48hrs. Medium from the upper (apical) and the lower (basal) chambers were then collected separately and measured for TX and PGI2 by their stable metabolites of TXB2 and 6-keto PGF1α by ELISA. Apical and basal releases of TX and PGI were also examined with apical exposure of TCs to arachidonic acid (AA)+/-aspirin at different concentrations. Villous tissue expressions for PGI synthase, TX synthase and TX (TP) receptor were examined by immunohistochemistry.Results-1) TXB2, but not 6-keto PGF1α, concentrations were significantly higher in the lower than in the upper chambers with both normal and PE TCs (p<0.01); 2) apical exposure of TCs to AA resulted in a significant increase in TX releases towards both the upper and the lower chambers in normal TCs (p<0.01), but only a significant increase in the upper chamber in PE TCs (p < 0.01); 3) aspirin could attenuate AA-induced TX release both in the upper and the lower chambers in normal, but not in PE, TCs (p<0.01), respectively; 4) there were no differences in 6-keto PGF1α productions both in normal and PE TCs treated with AA +/− aspirin; 5) intense staining of TX synthase and TP receptor was seen in syncytiotrophoblast layer, villous core vessels and stromal cells in preeclamptic placental tissue sections.Conclusion-Predominant basal release of TX together with intense staining of TX synthase and TP receptor in trophoblasts, stromal cells and villous core vessels are found in placentas from PE. We speculate if predominant basal release of TX by TCs occurs in vivo as we found in our in vitro culture condition, basal released TX may play a significant role in increased placental vasoconstriction such as in PE.

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Increased Superoxide Generation is Associated with Decreased Superoxide Dismutase Activity and mRNA Expression in Placental Trophoblast Cells in Pre-eclampsia

Cited by 210 publications

References 18 publications

Association of antioxidant vitamins and oxidative stress levels in pregnancy with infant growth during the first year of life

Association of antioxidant vitamins and oxidative stress levels in pregnancy with infant growth during the first year of life

Fetal programming of glucose–insulin metabolism

Predominant Basal Directional Release of Thromboxane, but not Prostacyclin, by Placental Trophoblasts from Normal and Preeclamptic Pregnancies

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