Increased survival of free fat grafts with platelet-rich plasma in rabbits

Fraga, Murillo Francisco Pires; Nishio, Ricardo Tadashi; Ishikawa, Ruy Saruwatari; Perin, Luí­s Fernando; Helene, Américo; Malheiros, Carlos Alberto

doi:10.1016/j.bjps.2010.07.003

Cited by 77 publications

(31 citation statements)

References 20 publications

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“…Although some successful clinical results were reported, 16,17 evidence supporting the application of PRP combined with fat grafts in soft tissue augmentation remains limited, as only a few basic research and preclinical studies in small animals have been conducted. 15,[18][19][20][21][22][23] Furthermore, no details on molecular mechanisms are addressed in the literature. In this review article, we discuss the possible molecular mechanisms of PRP in fat graft survival based on a review of the current literature.…”

Section: Introductionmentioning

confidence: 99%

“…Pires Fraga et al used a rabbit model to study the effect of PRP on fat graft survival. 19 Fat grafts were harvested from the dorsal scapular region and mixed with a near identical volume of autologous PRP, which was activated by CaCl 2 and thrombin. The mixtures were implanted in the subcutaneous ear of the rabbit model, and the results showed a significant increase in viable adipocytes and angiogenesis in the PRP group and an increased necrotic area and inflammation area in the saline group.…”

Section: Preclinical Studiesmentioning

confidence: 99%

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Application of Platelet-Rich Plasma and Platelet-Rich Fibrin in Fat Grafting: Basic Science and Literature Review

Marra

2014

Tissue Engineering Part B: Reviews

131

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Due to the natural properties of fat, fat grafting remains a popular procedure for soft tissue volume augmentation and reconstruction. However, clinical outcome varies and is technique dependent. Platelet-rich plasma (PRP) contains a-granules, from which multiple growth factors such as platelet-derived growth factor, transforming growth factor-b, vascular endothelial growth factor, and epidermal growth factor can be released after activation. In recent years, the scope of PRP therapies has extended from bone regeneration, wound healing, and healing of musculoskeletal injuries, to enhancement of fat graft survival. In this review, we focus on the definition of PRP, the different PRP preparation and activation methods, and growth factor concentrations. In addition, we discuss possible mechanisms for the role of PRP in fat grafting by reviewing in vitro studies with adipose-derived stem cells, preadipocytes, and adipocytes, and preclinical and clinical research. We also review platelet-rich fibrin, a so-called second generation PRP, and its slow-releasing biology and effects on fat grafts compared to PRP in both animal and clinical research. Finally, we provide a general foundation on which to critically evaluate earlier studies, discuss the limitations of previous research, and direct plans for future experiments to improve the optimal effects of PRP in fat grafting.

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Section: Introductionmentioning

confidence: 99%

Section: Preclinical Studiesmentioning

confidence: 99%

Application of Platelet-Rich Plasma and Platelet-Rich Fibrin in Fat Grafting: Basic Science and Literature Review

Marra

2014

Tissue Engineering Part B: Reviews

131

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“…In a rat model, one study found that activated PRP enhances fat graft retention as well as the survival, viability, and differentiation of adipocytes . Pires Fraga et al also found that PRP not only improved graft retention but also maintained a higher number of adipocytes and blood vessels with reduced necrosis and fibrosis in a rabbit model . In another rabbit model, Rodriguez‐Flores et al found that combination fat and PRP grafting reduced the inflammatory reaction and the accumulation of oil cysts (indicating fat necrosis) within adipocytes .…”

Section: Prp and Fat In Animal Studiesmentioning

confidence: 99%

The use of fat grafting and platelet‐rich plasma for wound healing: A review of the current evidence

Smith

Jell

Mosahebi

2018

International Wound Journal

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Fat grafting is becoming a common procedure in regenerative medicine because of its high content of growth factors and adipose derived stem cells (ADSCs) and the ease of harvest, safety, and low cost. The high concentration of ADSCs found in fat has the potential to differentiate into a wide range of wound‐healing cells including fibroblasts and keratinocytes as well as demonstrating proangiogenic qualities. This suggests that fat could play an important role in wound healing. However retention rates of fat grafts are highly variable due in part to inconsistent vascularisation of the transplanted fat. Furthermore, conditions such as diabetes, which have a high prevalence of chronic wounds, reduce the potency and regenerative potential of ADSCs. Platelet‐rich plasma (PRP) is an autologous blood product rich in growth factors, cell adhesion molecules, and cytokines. It has been hypothesised that PRP may have a positive effect on the survival and retention of fat grafts because of improved proliferation and differentiations of ADSCs, reduced inflammation, and improved vascularisation. There is also increasing interest in a possible synergistic effect that PRP may have on the healing potential of fat, although the evidence for this is very limited. In this review, we evaluate the evidence in both in vitro and animal studies on the mechanistic relationship between fat and PRP and how this translates to a benefit in wound healing. We also discuss future directions for both research and clinical practice on how to enhance the regenerative potential of the combination of PRP and fat.

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“…Some clinicians advocate the isolation of these cells in the stromovascular fraction to augment the lipoaspirate in fat grafting (Yoshimura et al, 2009), based on the notion that adipose-derived mesenchymal stem cells in the stromovascular fraction secrete angiogenic growth factors, which may increase graft survival. Other growth factors such as insulin, insulin-like growth factor-1 (IGF-1; Yuksel et al, 2000), and platelet-rich plasma (Nakamura et al, 2010; Pires Fraga et al, 2010) have also been added to fat grafts to improve retention, with greater final fat graft weight, and vascularization as compared with untreated grafts in animal models.…”

Section: Stable Restoration Of Facial Contour By Transplantation Of Amentioning

confidence: 99%

Regenerative Strategies for Craniofacial Disorders

Garland¹,

Pomerantz²

2012

Front. Physio.

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Craniofacial disorders present markedly complicated problems in reconstruction because of the complex interactions of the multiple, simultaneously affected tissues. Regenerative medicine holds promise for new strategies to improve treatment of these disorders. This review addresses current areas of unmet need in craniofacial reconstruction and emphasizes how craniofacial tissues differ from their analogs elsewhere in the body. We present a problem-based approach to illustrate current treatment strategies for various craniofacial disorders, to highlight areas of need, and to suggest regenerative strategies for craniofacial bone, fat, muscle, nerve, and skin. For some tissues, current approaches offer excellent reconstructive solutions using autologous tissue or prosthetic materials. Thus, new “regenerative” approaches would need to offer major advantages in order to be adopted. In other tissues, the unmet need is great, and we suggest the greatest regenerative need is for muscle, skin, and nerve. The advent of composite facial tissue transplantation and the development of regenerative medicine are each likely to add important new paradigms to our treatment of craniofacial disorders.

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Increased survival of free fat grafts with platelet-rich plasma in rabbits

Cited by 77 publications

References 20 publications

Application of Platelet-Rich Plasma and Platelet-Rich Fibrin in Fat Grafting: Basic Science and Literature Review

Application of Platelet-Rich Plasma and Platelet-Rich Fibrin in Fat Grafting: Basic Science and Literature Review

The use of fat grafting and platelet‐rich plasma for wound healing: A review of the current evidence

Regenerative Strategies for Craniofacial Disorders

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