Increased susceptibility of algal symbionts to photo-inhibition resulting from the perturbation of coral gastrodermal membrane trafficking

Chen, Chii-Shiarng; Yeh, Shih-Pin; Wang, Li-Hsueh; Li, Hsing-Hui; Chen, Uang Wan-Nan

doi:10.1007/s11427-012-4345-4

Cited by 11 publications

(19 citation statements)

References 45 publications

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“…Lesser, 1996;Jantzen et al, 1998;Blackstone, 2001;Franklin et al, 2004;Chen et al, 2012). This probe requires incubation, typically for 1 h in the dark.…”

Section: Measures Of Ros Rns and Nad(p)h In Host Stolonsmentioning

confidence: 99%

Physiological correlates of symbiont migration during bleaching of two octocoral species

Netherton

Scheer

Morrison

et al. 2014

Journal of Experimental Biology

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Perturbed colonies of Phenganax parrini and Sarcothelia sp. exhibit migration of symbionts of Symbiodinium spp. into the stolons. Densitometry and visual inspection indicated that polyps bleached while stolons did not. When migration was triggered by temperature, light and confinement, colonies of Sarcothelia sp. decreased rates of oxygen formation in the light (due to the effects of perturbation on photosynthesis and respiration) and increased rates of oxygen uptake in the dark (due to the effects of perturbation on respiration alone). Colonies of P. parrini, by contrast, showed no significant changes in either aspect of oxygen metabolism. When migration was triggered by light and confinement, colonies of Sarcothelia sp. showed decreased rates of oxygen formation in the light and increased rates of oxygen uptake in the dark, while colonies of P. parrini maintained the former and increased the latter. During symbiont migration into their stolons, colonies of both species showed dramatic increases in reactive oxygen species (ROS), as visualized with a fluorescent probe, with stolons of Sarcothelia sp. exhibiting a nearly immediate increase of ROS. Differences in symbiont type may explain the greater sensitivity of colonies of Sarcothelia sp. Using fluorescent probes, direct measurements of migrating symbionts in the stolons of Sarcothelia sp. showed higher levels of reactive nitrogen species and lower levels of ROS than the surrounding host tissue. As measured by native fluorescence, levels of NAD(P)H in the stolons were unaffected by perturbation. Symbiont migration thus correlates with dramatic physiological changes and may serve as a marker for coral condition.

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“…Lesser, 1996;Jantzen et al, 1998;Blackstone, 2001;Franklin et al, 2004;Chen et al, 2012). This probe requires incubation, typically for 1 h in the dark.…”

Section: Measures Of Ros Rns and Nad(p)h In Host Stolonsmentioning

confidence: 99%

Physiological correlates of symbiont migration during bleaching of two octocoral species

Netherton

Scheer

Morrison

et al. 2014

Journal of Experimental Biology

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“…Zooxanthellates DNA was extracted from the coral fragments, and the clades were successively identified according to the internal transcribed spacer rDNA sequence [21]. After the pre–treatment procedure, selected clones were sequenced and the obtained sequences were searched for and aligned in the NCBI GenBank Database with BLAST (http://www.ncbi.nlm.nih.gov/).…”

Section: Methodsmentioning

confidence: 99%

Cellular Membrane Accommodation to Thermal Oscillations in the Coral Seriatopora caliendrum

et al. 2014

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In the present study, the membrane lipid composition of corals from a region with tidally induced upwelling was investigated. The coral community is subject to strong temperature oscillations yet flourishes as a result of adaptation. Glycerophosphocholine profiling of the dominant pocilloporid coral, Seriatopora caliendrum, was performed using a validated method. The coral inhabiting the upwelling region shows a definite shift in the ratio of lipid molecular species, covering several subclasses. Mainly, the coral possesses a higher percentage of saturated, monounsaturated and polyunsaturated plasmanylcholines and a lower percentage of polyunsaturated phosphatidylcholines. Higher levels of lyso–plasmanylcholines containing saturated or monounsaturated fatty acid chains were also revealed in coral tissue at the distal portion of the branch. Based on the physicochemical properties of these lipids, we proposed mechanisms for handling cellular membrane perturbations, such as tension, induced by thermal oscillation to determine how coral cells are able to spontaneously maintain their physiological functions, in both molecular and physical terms. Interestingly, the biochemical and biophysical properties of these lipids also have beneficial effects on the resistance, maintenance, and growth of the corals. The results of this study suggest that lipid metabolic adjustment is a major factor in the adaption of S. caliendrum in upwelling regions.

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“…During the initial step of the endosymbiotic process, SGCs are involved in the recognition and phagocytosis of Symbiodinium 16, 17 . Proteomic analyses have demonstrated that SGC plasma membrane proteins are involved in the molecular chaperone/stress response, cytoskeletal remodeling, and energy metabolism 18 .…”

Section: Introductionmentioning

confidence: 99%

Generation of clade- and symbiont-specific antibodies to characterize marker molecules during Cnidaria-Symbiodinium endosymbiosis

Huang

Lin

et al. 2017

Sci Rep

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The endosymbiosis between cnidarians and dinoflagellates is responsible for the formation of coral reefs. Changes in molecules have been identified during the process of cnidaria-Symbiodinium endosymbiosis. However, the complexity of the molecular interaction has prevented the establishment of a mechanistic explanation of cellular regulation in this mutualistic symbiosis. To date, no marker molecules have been identified to specifically represent the symbiotic status. Because the endosymbiotic association occurs in the symbiotic gastrodermal cells (SGCs), whole cells of isolated SGCs were used as an antigen to generate monoclonal antibodies (mAb) to screen possible molecular candidates of symbiotic markers. The results showed that one of the generated monoclonal antibodies, 2–6F, specifically recognized clade C symbiotic Symbiodinium but not its free-living counterpart or other Symbiodinium clades. The expression levels of 2–6F mAb-recognized proteins are highly correlated with the symbiotic status, and these proteins were characterized as N-linked glycoproteins via treatment with peptide N-glycosidase F. Furthermore, their glycan moieties were markedly different from those of free-living Symbiodinium, potentially suggesting host regulation of post-translational modification. Consequently, the 2–6F mAb can be used to detect the symbiotic state of corals and investigate the complex molecular interactions in cnidaria-Symbiodinium endosymbiosis.

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Increased susceptibility of algal symbionts to photo-inhibition resulting from the perturbation of coral gastrodermal membrane trafficking

Cited by 11 publications

References 45 publications

Physiological correlates of symbiont migration during bleaching of two octocoral species

Physiological correlates of symbiont migration during bleaching of two octocoral species

Cellular Membrane Accommodation to Thermal Oscillations in the Coral Seriatopora caliendrum

Generation of clade- and symbiont-specific antibodies to characterize marker molecules during Cnidaria-Symbiodinium endosymbiosis

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