Increased susceptibility of erythrocytes to in vitro peroxidation in acute Trypanosoma brucei infection of mice

Igbokwe, I.O.; Esievo, K. A. N.; Saror, D.I.; Obagaiye, O.K.

doi:10.1016/0304-4017(94)90070-1

Cited by 38 publications

(25 citation statements)

References 9 publications

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“…Plasma lipids are also sensitive to peroxidation [24]. Parallel to the current study, earlier researches demonstrated higher level of MDA in T. brucei infected mice [25], humans infected with Trypanosoma cruzi [26][27][28] and in naturally infected camel with trypanosoma evansi [13]. In healthy aerobic organisms production of free radicals is approximately balanced with antioxidant defense system [29].…”

Section: Discussionsupporting

confidence: 58%

Blood Picture and Selected Oxidative Stress Biomarkers in Dromedary Camels Naturally Infected With Trypanosoma Evansi

Eljalii

El-Deeb

Fouda

et al. 2015

International Journal of Veterinary Sciences Research

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Section: Discussionsupporting

confidence: 58%

Blood Picture and Selected Oxidative Stress Biomarkers in Dromedary Camels Naturally Infected With Trypanosoma Evansi

Eljalii

El-Deeb

Fouda

et al. 2015

International Journal of Veterinary Sciences Research

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“…Trypanosome brucei cause anaemia through disruption of erythrocytes membrane integrity (Huan et al, 2000). Erythrocytic peroxidation has also been reported to be one of the factors which play an important role in the pathogenesis of anaemia in mice infected with T. brucei (Igbokwe et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…This has been reported to due to inflammation and necrosis in the infected host particularly in organs like liver, kidney muscle and even heart (Losos and Ikede, 1972). This is possible due to the ability of T. brucei to invade soft tissue especially the liver, kidney and heart thereby localizing and causing tissue damage which subsequently leads to the release of these enzymes from damaged tissue (Igbokwe et al, 1994).…”

Section: Discussionmentioning

confidence: 99%

Crude methanolic extract of <em>Moringa oleifera</em> leaves improves the efficacy of diminazene aceturate in the treatment of trypanosome infected rats

et al. 2017

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A trypanocidal efficacy study of Moringa oleifera leaf supplement alone and in combination with diminazene aceturate on Wistar rats was conducted using Trypanosoma brucei. Thirty five rats were randomly allotted into seven groups (A-G) with include five rats each. Groups B and C were treated with methanolic extract of M. oleifera leaves at 200mg/kg for 14 days prior to infection. The E and F were treated with M. oleifera for 90 days at 200mg/kg prior to infection. Group A and G are negative and positive controls, respectively. All rats in groups, B -G were individually infected with 3x10 6 of Trypanosoma brucei per ml of blood and the prepatent period was monitored from the second day. At day five post infection, infected rats in group C, D and F were treated with diminazene aceturate at 7mg/kg while those in group B and E treated with M. oleifera leaf extract. Parasites were cleared within 72 hours post treatment from blood of rats in groups C and F which were treated with both M. aoleifera and diminazine aceturate. However, it took 96 hours post treatment for parasites to be cleared from rats in group D which was treated only with diminazene aceturate. There were significant (P<0.05) increase in the erythrocyte count, haematocrit and mean corpuscular volume (MCV) in M. oleifera treated groups. Mean Corpuscular Haemoglobin (MCH) and Mean Corpuscular Haemoglobin Concentration (MCHC) decreased significantly (P<0.05) in groups E, F and G. There was neutropenia in groups E, F and G when compared with the negative control, while other leucocytes manifested leukocytosis. Serum chemistry showed that all groups had significantly increased (P<0.05) globulin, blood urea nitrogen (BUN) and liver enzymes when compared with the negative control group "A". Histopathological findings showed that congestion in the spleen and lymph nodes were reduced in the treated groups when compared to the untreated groups. Conclusively, our study gives credence that M. oleifera does not possess trypanocidal effect but however could be coadministered as a supportive.

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“…The lipid peroxidation in the erythrocyte membrane has been identified as one of the most important factors in the pathogenesis of anemia in parasitic infections, as seen in rats with acute trypanosomosis by T. brucei (Igbokwe et al 1994), dogs infected with Babesia gibsoni (Chaudhuri et al 2008), and cattle infected with Babesia bigemina (Saleh 2009a). The phospholipid peroxidation is evaluated by concentrations of malondialdehyde (MDA), and the assessment of thiobarbituric acid reactive substances (TBARS) probably is the most commonly applied method for the measurement of lipid peroxidation (Esterbauer 1993).…”

Section: Introductionmentioning

confidence: 99%

Lipid peroxidation in cats experimentally infected with Trypanosoma evansi

et al. 2009

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The objective of this study was to evaluate the lipid peroxidation and the susceptibility of erythrocytes to in vitro peroxidation as indicators of oxidative damage in erythrocytes and their roles in the pathogenesis of anemia during experimental Trypanosoma evansi infection in cats. Animals were divided into two groups: control and infected with T. evansi. Seven cats were infected with 10(8) trypomastigotes each, and parasitemia was estimated daily for 49 days by microscopic examination of smears. Hematological and biochemical parameters were evaluated for monitoring of the disease. Plasma lipid peroxidation (Thiobarbituric Acid Reactive Substances (TBARS)) and the susceptibility of erythrocytes to in vitro peroxidation were evaluated. Blood samples for analysis were collected at days 21 and 49 post-inoculation. TBARS level, indicated by MDA concentration, was higher in the infected group than in the control group in both analyzed periods, as well as the in vitro erythrocyte peroxidation (P < 0.001). The infected cats had variable degrees of regenerative anemia, which could be explained by the damage in erythrocyte membrane caused by lipid peroxidation.

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Increased susceptibility of erythrocytes to in vitro peroxidation in acute Trypanosoma brucei infection of mice

Cited by 38 publications

References 9 publications

Blood Picture and Selected Oxidative Stress Biomarkers in Dromedary Camels Naturally Infected With Trypanosoma Evansi

Blood Picture and Selected Oxidative Stress Biomarkers in Dromedary Camels Naturally Infected With Trypanosoma Evansi

Crude methanolic extract of <em>Moringa oleifera</em> leaves improves the efficacy of diminazene aceturate in the treatment of trypanosome infected rats

Lipid peroxidation in cats experimentally infected with Trypanosoma evansi

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