Increased susceptibility of gentamincin-resistantPseudomonas aeruginosa to human sera

Clark, Richard B.; Hu, Daniel H.; Janda, J. Michael; Hostetter, Marcia K.

doi:10.1007/bf01568512

Cited by 6 publications

(1 citation statement)

References 24 publications

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“…These isolates were recovered from different patients at The Mount Sinai Hospital in New York, NY (four isolates), from the Saint Joseph Hospital in Omaha, Nebraska (five isolates), and from the Veterans Administration Medical Center in Omaha (one isolate). All strains were identified as P. aeruginosa by previously described methods [5][6][7] and showed the ArGrT s antibiogram when tested by the NCCLS disk diffusion procedure [25]. Multiple passaging was avoided to prevent possible loss of antibiotic resistance.…”

Section: Methodsmentioning

confidence: 99%

Synergistic activity of aminoglycoside-beta-lactam combinations against Pseudomonas aeruginosa with an unusual aminoglycoside antibiogram

Clark

Pakiz

Hostetter

1990

Med Microbiol Immunol

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The bactericidal activity of aminoglycosides alone and in combination with various beta-lactams was studied by the time-kill technique against ten Pseudomonas aeruginosa isolates with an unusual antibiogram (amikacin-resistant, gentamicin-resistant, tobramycin-susceptible [ArGrTs]). Previous studies have indicated that ArGrTs isolates are moderately resistant to all aminoglycosides and many are multiply resistant to beta-lactams. Aminoglycoside-beta-lactam combinations showed infrequent synergistic (16%) or enhanced killing (12%) against the ArGrTs isolates. Synergistic activity, when present, was more likely to occur with tobramycin and amikacin than with gentamicin, even though these differences were not statistically significant. Antibiotic resistance patterns were not predictive of synergy or enhanced killing. Systemic infections produced by ArGrTs isolates that are multiply resistant to the beta-lactams may not respond to combination therapy with an aminoglycoside and beta-lactam. Alternative treatment with polymyxin B or a quinolone may be required for these infections.

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Section: Methodsmentioning

confidence: 99%

Synergistic activity of aminoglycoside-beta-lactam combinations against Pseudomonas aeruginosa with an unusual aminoglycoside antibiogram

Clark

Pakiz

Hostetter

1990

Med Microbiol Immunol

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Susceptibility to R-pyocins of Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients

Redero

López-Causapé

Aznar

et al. 2018

Journal of Antimicrobial Chemotherapy

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Adaptive resistance to aminoglycoside antibiotics in Pseudomonas aeruginosa

Gilleland

Gibson

et al. 1989

Journal of Medical Microbiology

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Summary. Aminoglycoside-resistant variants of Pseudornonas aeruginosa strain P A 0 1 were readily selected by culturing the organism in medium containing increasing concentrations of gentamicin, tobramycin or amikacin until the strains were growing in a concentration of drug 128-fold greater than the minimal inhibitory concentration for the sensitive parent strain. These resistant strains exhibited characteristics previously associated with the impermeability type of resistance mechanism, i.e., they grew more slowly than the parent strain, the resistance was unstable in the absence of the antibiotic, and adaptation to one of the antibiotics conferred crossresistance to other aminoglycosides. The adapted strains grew, with minimal morphological alterations, in concentrations of the various aminoglycosides that normally produced cell envelope damage, misshapen and filamentous cell formation, and cell lysis in the sensitive strain. Neither protein H1 nor phospholipid alterations appear to play a significant role in adaptive resistance to aminoglycoside antibiotics in this model system. The acquisition of adaptive resistance to the aminoglycoside antibiotics did not confer resistance to polymyxin B, another cationic antibiotic which is thought to share binding sites within the outer membrane with the aminoglycosides.

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Increased susceptibility of gentamincin-resistantPseudomonas aeruginosa to human sera

Cited by 6 publications

References 24 publications

Synergistic activity of aminoglycoside-beta-lactam combinations against Pseudomonas aeruginosa with an unusual aminoglycoside antibiogram

Synergistic activity of aminoglycoside-beta-lactam combinations against Pseudomonas aeruginosa with an unusual aminoglycoside antibiogram

Susceptibility to R-pyocins of Pseudomonas aeruginosa clinical isolates from cystic fibrosis patients

Adaptive resistance to aminoglycoside antibiotics in Pseudomonas aeruginosa

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