Increased Susceptibility of Radiation-Induced Intestinal Apoptosis in SMP30 KO Mice

Abstract

Recently, senescence marker protein-30 (SMP30) knockout (KO) mice have been reported to be susceptible to apoptosis, however, the role of SMP30 has not been characterized in the small intestine. The aim of the present study is to investigate the role of SMP30 in the process of spontaneous and γ-radiation-induced apoptosis in mouse small intestine. Eight-week-old male wild-type (WT) mice and SMP30 KO mice were examined after exposure to 0, 1, 3, 5, and 9 Gy of γ-radiation. Apoptosis in the crypts of the small intestine increased in the 0 to 5 Gy radiated SMP30 KO and WT mice. Radiation-induced apoptosis and the BAX/Bcl-2 ratio in the SMP30 KO mice were significantly increased in comparison to each identically treated group of WT mice (p < 0.05). The levels of spontaneous apoptosis in both WT and KO mice were similar (p > 0.05), indicating that increased apoptosis of crypt cells of SMP30 KO by irradiation can be associated with SMP30 depletion. These results suggested that SMP30 might be involved in overriding the apoptotic homeostatic mechanism in response to DNA damage.

Effects of dose-dependent response to gamma radiation on circumvallate papilla by expression of caspase-3 in vivo

“…Although the signs of cell death were observed in all the irradiation doses, nevertheless mild effects were seen in the dose 2 Gy in comparison to 4 and 6 Gy. This was consistent with Bishay et al, 2000 , Goo et al, 2013 who reported dose dependent cell death with more observable signs in doses over 2 Gy. Furthermore, this result coincides with preceding studies that recommended fractionation of radiotherapy dose into separated doses claiming that 2 Gy is a safe dose to the normal tissues in the field of radiation ( Vissink et al, 2003 ) .…”

Caffeic acid phenethyl ester attenuates ionize radiation-induced intestinal injury through modulation of oxidative stress, apoptosis and p38MAPK in rats

“…Excessive apoptosis is one of the main etiological factors contributing to intestinal injury after radiation exposure (Goo et al, 2013). Similar findings were observed in this study.…”

A multi-component herbal preparation, STW 5, shows anti-apoptotic effects in radiation induced intestinal mucositis in rats

“…Excessive apoptosis is one of the main etiological factors contributing to intestinal injury after radiation exposure (Goo et al, 2013). Intestinal stem cells are prone to apoptosis caused by radiation exposure because the intestinal epithelium is one of the fastest proliferating tissues in the body (Matsuu-Matsuyama et al, 2006).…”