Increased synthesis of folate transporters regulates folate transport in conditions of ethanol exposure and folate deficiency

Thakur, Shilpa; More, Deepti; Rahat, Beenish; Khanduja, Krishan Lal; Kaur, Jyotdeep

doi:10.1007/s11010-015-2577-x

Cited by 7 publications

(10 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Folate deficiency also results in decreased half-lives of these two genes [35]. In the present study, no correlation between gene expression and folate concentration was found in tumour tissue of untreated patients; however, the gene expression levels of GGH and SLC46A1/PCFT in tumour were negatively correlated with 5-MTHF in plasma (i.e.…”

Section: Discussioncontrasting

confidence: 66%

“…Several previous studies have shown that folate deficiency results in a significant upregulation of folate transport genes such as SLC19A1/RFC-1 and SLC46A1/PCFT [ 33 , 34 ]. Folate deficiency also results in decreased half-lives of these two genes [ 35 ]. In the present study, no correlation between gene expression and folate concentration was found in tumour tissue of untreated patients; however, the gene expression levels of GGH and SLC46A1/PCFT in tumour were negatively correlated with 5-MTHF in plasma (i.e.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Relationship between folate concentration and expression of folate-associated genes in tissue and plasma after intraoperative administration of leucovorin in patients with colorectal cancer

Taflin

Odin

Derwinger

et al. 2018

Cancer Chemother Pharmacol

View full text Add to dashboard Cite

PurposeThe aim of study was to investigate the relationship between folate concentration and expression of folate-associated genes in tumour, mucosa and plasma of patients with colorectal cancer, after intraoperative administration of bolus leucovorin (LV).MethodsEighty patients were randomized into four groups to receive 0, 60, 200, or 500 mg/m2 LV, respectively. Tissue and plasma folate concentrations were assessed by LC–MS/MS. Gene expression of ABCC3/MRP3, FPGS, GGH, MTHFD1L, SLC46A1/PCFT, and SLC19A1/RFC-1 was determined using quantitative PCR.ResultsThe folate concentration in tumour increased with increasing dosage of LV. Half of the patients treated with 60 mg/m2 did not reach a level above the levels of untreated patients. A significant correlation between folate concentration in tumour and mucosa was found in untreated patients, and in the group treated with 60 mg/m2 LV. The 5-MTHF/LV ratio correlated negatively with folate concentration in mucosa, whereas a positive correlation was found in tumour of patients who received 200 or 500 mg/m2 LV. A positive correlation was found between folate concentration and expression of all genes, except MTHFD1L, in patients who received LV. There was a negative correlation between 5-MTHF concentration in plasma of untreated patients and expression of GGH and SLC46A1/PCFT in tumour.ConclusionsThe results indicate the possibility of using the individual plasma 5-MTHF/LV ratio after LV injection as a surrogate marker for tissue folate concentration. Expression of several folate-associated genes is associated with folate concentration in tissue and plasma and may become useful when predicting response to LV treatment.Electronic supplementary materialThe online version of this article (10.1007/s00280-018-3690-9) contains supplementary material, which is available to authorized users.

show abstract

Section: Discussioncontrasting

confidence: 66%

Section: Discussionmentioning

confidence: 99%

Relationship between folate concentration and expression of folate-associated genes in tissue and plasma after intraoperative administration of leucovorin in patients with colorectal cancer

Taflin

Odin

Derwinger

et al. 2018

Cancer Chemother Pharmacol

View full text Add to dashboard Cite

show abstract

“…J. Kaur (Institute of Medical Education and Research, India) described in the presentation “ Multiple Regulatory Mechanisms Control the Expression of Folate Transporters in Conditions of Ethanol Exposure and Folate Deficiency” her research on the regulation of the major folate transport systems in response to folate deficiency [23]. Folate deficiency can occur in both developing and well-developed countries with inadequate dietary folate intake and severe alcoholism as the most frequent causes.…”

Section: Physiology and Pathophysiology Of Folate Receptors And Transmentioning

confidence: 99%

Folate receptors and transporters: biological role and diagnostic/therapeutic targets in cancer and other diseases

Frigerio

Bizzoni

Jansen

et al. 2019

J Exp Clin Cancer Res

View full text Add to dashboard Cite

Folate receptors and transporters and one-carbon metabolism continue to be important areas of study given their essential roles in an assortment of diseases and as targets for treatment of cancer and inflammation. Reflecting this, every 2 years, the Folate Receptor Society organizes an international meeting, alternating between North America and Europe, where basic and translational scientists, clinical oncologists and rheumatologists from both academia and industry convene in an informal setting. The 7th International Symposium on Folate Receptors and Transporters was held in Sant’Alessio Siculo (ME), Taormina, Italy from 1st to 5th of October 2018, organized by Dr. Mariangela Figini from Fondazione IRCCS Istituto Nazionale dei Tumori, Milan. Following the format of previous meetings, more than 50 scientists from 9 different countries attended the 2018 meeting to share ongoing developments, discuss current research challenges and identify new avenues in basic and translational research. An important feature of this meeting was the participation of young investigators and trainees in this area, two (A. Dekhne and N. Verweij) of whom were awarded fellowships to attend this meeting as a recognition of the high scientific quality of their work. This report provides a synopsis of the highlights presented in the following sessions: Barton Kamen Lecture; Targeting one-carbon metabolism in cytosol and mitochondria; Structure and biology of the one-carbon solute transporters; Physiology and pathophysiology of folate receptors and transporters; Folate receptors for targeting tumors and inflammatory diseases; Conventional and new anti-folate drugs for treating inflammatory diseases and cancer; Imaging; Ongoing clinical trials; and Chimeric Antigen Receptor cell therapies of cancer.

show abstract

“…Although we confirmed that the ovary FA concentration in our FA-deficient mice was very low during oocyte meiosis, frequency of abnormal oocytes did not increase in the FA-deficient group. This result suggests that feeding the FA-deficient does not affect the oocyte FA concentration, because the RFC1 expression in HEK293 cells, liver and intestine is known to be increased by FA deficiency (26)(27)(28). However, there is no report about effect of FA deficiency on RFC1 expression in the ovary or oocyte.…”

Section: Discussionmentioning

confidence: 95%

“…RFC1 expresses in peripheral tissues and RFC1 has high affinity for the reduced FA such as 5-methyl-THF, 5-folmyl-THF (25). Interestingly, the RFC1 expression level is promoted in an FA-deficient environment to maintain the FA homeostasis (26). RFC1 expression is strong in the ovary of hens (22) and also in the cumulus-oocyte complex of mice (24).…”

Section: Discussionmentioning

confidence: 99%

Folic Acid Deficiency Does Not Adversely Affect Oocyte Meiosis in Mice

Tsuji

Noguchi

Nakamura

et al. 2016

J Nutr Sci Vitaminol

View full text Add to dashboard Cite

Summary Spindle defect and chromosome misalignment occuring in oocyte meiosis induce nondisjunction. Nondisjunction causes Down syndrome, also known as trisomy 21. Folic acid (FA) is an essential nutrient composition for fetal growth and development. It has been reported that FA nutritional status is associated with the risk of Down syndrome. However, to our knowledge, little is known about the effect of FA deficiency on abnormal oocytes (spindle defects, chromosome misalignments and immature oocyte) in vivo. In the present study, we investigate the effects of FA deficiency on oocyte meiosis in female mice. In order to induce FA deficiency in mice, female Crl:CD1 mice were fed a FA-free diet for 58 d. The diet also contained an antibiotic which has functions on limiting FA formation by intestinal microorganisms. The level of FA deficiency was determined by measuring the concentration of FA in the liver, hemocyte, uterus, ovary, and urine. FA concentrations in these samples from the FA-deficient group were 50-90% lower. Despite this, the frequency of abnormal oocytes was no different between the FA-deficient and control groups (20.0% vs 14.6%). According to the past research, FA transporter was strongly expressed in oocytes. Hence, it is possible that FA-free diets may not affect the concentration of oocyte FA in mice. To sum up these data, our study concluded that FA deficiency did not adversely affect oocyte meiosis.

show abstract

Increased synthesis of folate transporters regulates folate transport in conditions of ethanol exposure and folate deficiency

Cited by 7 publications

References 47 publications

Relationship between folate concentration and expression of folate-associated genes in tissue and plasma after intraoperative administration of leucovorin in patients with colorectal cancer

Relationship between folate concentration and expression of folate-associated genes in tissue and plasma after intraoperative administration of leucovorin in patients with colorectal cancer

Folate receptors and transporters: biological role and diagnostic/therapeutic targets in cancer and other diseases

Folic Acid Deficiency Does Not Adversely Affect Oocyte Meiosis in Mice

Contact Info

Product

Resources

About