Increased Tea Saponin Content Influences the Diversity and Function of Plantation Soil Microbiomes

Zhang, Shouke; Kong, Junqia; Chen, Longfei; Guo, Kai; Zhou, Xudong

doi:10.1128/spectrum.02324-21

Cited by 12 publications

(7 citation statements)

References 49 publications

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“…In conclusion, we demonstrated differential inhibition of ecologically important fungi isolated from the rhizosphere of the perennial bioenergy crop by saponins and diterpenoids -the metabolites preferentially accumulated in the upland and lowland switchgrass roots, respectively. Results of this study add to published work demonstrating that the antimicrobial properties of plant specialized metabolites influence microbiome assembly in crops as diverse as Panax notoginseng (Wei et al, 2022), Camellia oleifera (Zhang et al, 2022), Lilium Oriental (Liu et al, 2011), and Zea mays (Murphy et al, 2021). Together with these previous studies, our results suggest that ecotypic differences in switchgrass terpene specialized metabolites might differentially influence soil fungal microbiome activities.…”

Section: B -D and Fig S2)supporting

confidence: 82%

Identification of anti-fungal bioactive terpenoids from the bioenergy crop switchgrass (Panicum virgatum)

Chou

Bonito

et al. 2023

Preprint

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Plant derived bioactive small molecules have attracted attention of scientists across fundamental and applied scientific disciplines. We seek to understand the influence of these phytochemicals on functional phytobiomes. Increased knowledge of specialized metabolite bioactivities could inform strategies for sustainable crop production. Our recent investigations of metabolomes of the upland and lowland ecotypes of the bioenergy crop switchgrass revealed large differences in types and abundances of terpenoid specialized metabolites. We hypothesized that - consistent with accumulating evidence that switchgrass genotype impacts microbiome assembly - differential terpenoid accumulation contributes to switchgrass ecotype-specific microbiome composition. An initial plate-based disc diffusion screen of 18 switchgrass root derived fungal isolates revealed differential responses to upland- and lowland-isolated metabolites. To identify specific fungal growth-modulating metabolites, we tested fractions from root extracts on three ecologically important fungal isolates - Linnemania elongata, Trichoderma sp. and Fusarium sp. Saponins and diterpenoids were identified as the most prominent antifungal metabolites. Finally, analysis of liquid chromatography-purified terpenoids revealed fungal inhibition structure - activity relationships (SAR). Saponin antifungal activity was primarily determined by the number of sugar moieties - saponins glycosylated at a single core position were inhibitory whereas saponins glycosylated at two core positions were inactive. Saponin core hydroxylation and acetylation were also associated with reduced activity. Diterpenoid activity required the presence of an intact furan ring for strong fungal growth inhibition. This study demonstrates that switchgrass genotype-specific metabolites differentially inhibit fungal isolates from the root and rhizosphere, supporting the hypothesis that these molecules contribute to microbiome assembly and function.

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Section: B -D and Fig S2)supporting

confidence: 82%

Identification of anti-fungal bioactive terpenoids from the bioenergy crop switchgrass (Panicum virgatum)

Chou

Bonito

et al. 2023

Preprint

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“…Different fertilization practices can affect soil microbial activities (Hicks et al, 2020;Zhang et al, 2022). Fertilization alters soil fertility by affecting nutrient levels, such as those of soil organic carbon (C) and total nitrogen (N), which directly composition of soil microbial communities (Sradnick et al, 2013;Chen et al, 2015;Zhang et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Effects of long-term application of organic manure and chemical fertilizer on soil properties and microbial communities in the agro-pastoral ecotone of North China

Gao

Duan

Zhang

et al. 2022

Front. Environ. Sci.

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Long-term irrational fertilizer inputs affect soil nutrients conditions in the agro-pastoral ecotone of North China. However, the mechanisms by which biotic and abiotic factors are affected by different fertilizer types remain unclear. A 16-year, long-term fertilization experiment was conducted to explore how soil physicochemical properties and microbial communities respond to different fertilizer types at an experimental site in North China. The key environmental factors that drove changes in soil microbial communities were also determined. In September 2019, soils were collected from plots of four fertilizer treatments: 1) non-fertilization control (CK), 2) chemical fertilization only (CF), 3) organic manure fertilization only (M), and 4) chemical fertilization plus organic manure (CFM). Compared with CK, soil organic matter, total nitrogen, available nitrogen, available phosphorus, and available potassium contents were higher in M and CFM, whereas soil pH was significantly lower in CF. Abundances of dominant soil bacterial phyla Proteobacteria, Bacteroidetes, and Gemmatimonadetes were higher in M and CFM than CK. Abundances of dominant soil fungal phyla Ascomycota was lower in CFM than in other treatments. The pathogenic fungi Fusarium, Paramyrothecium, Cladosporium, and Alternaria had the highest abundances in CK and CF, whereas abundances of the beneficial fungi Mortierella were significantly higher in M and CFM than in CF and CK. According to partial least squares path modeling, differences in fertilizer types had direct positive effects on fungal communities but little effect on bacterial communities. Overall, CFM maintained higher soil fertility and a healthy ecosystem because it increased beneficial microorganisms and inhibited pathogenic microorganisms, whereas CF increased the risk of crop infection with soil-borne diseases. The study provided a better understanding of how long-term fertilization affects microbial community composition and their associated ecosystem functions.

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“…Overall, we demonstrated that a host-specific pest CW possesses a gut microbiome that is significantly associated with TS degradation ( 36 ). We also demonstrated that the Acinetobacter genus from the CW gut microbiome has significant linear relation with the TS content in Camellia fruits.…”

Section: Discussionmentioning

confidence: 97%

Specific Enriched Acinetobacter in Camellia Weevil Gut Facilitate the Degradation of Tea Saponin: Inferred from Bacterial Genomic and Transcriptomic Analyses

Huang

He³

et al. 2022

Microbiol Spectr

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Increased Tea Saponin Content Influences the Diversity and Function of Plantation Soil Microbiomes

Cited by 12 publications

References 49 publications

Identification of anti-fungal bioactive terpenoids from the bioenergy crop switchgrass (Panicum virgatum)

Identification of anti-fungal bioactive terpenoids from the bioenergy crop switchgrass (Panicum virgatum)

Effects of long-term application of organic manure and chemical fertilizer on soil properties and microbial communities in the agro-pastoral ecotone of North China

Specific Enriched Acinetobacter in Camellia Weevil Gut Facilitate the Degradation of Tea Saponin: Inferred from Bacterial Genomic and Transcriptomic Analyses

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