The aim of this study was to evaluate possible effects of temperature or microcirculation changes on the acupoint UB.67 during acupressure, needle or laserneedle stimulation at the acupoint UB.2, as described for acupressure by other authors. We investigated eleven healthy volunteers with a mean age SD of 30.0 7.2 years. The measurement equipment consisted of noncontact (infrared camera ThermaCAM P640, Flir Systems Inc., Portland, Oregon, USA) and contact (laser Doppler flowmetry and temperature device DRT4 from Moor Instruments Ltd., Millwey, Axminster, U.K.) methods. The results showed that there were no significant (one-way repeated measures ANOVA) changes during any of the stimulation methods used. We conclude that stimulation at the acupoint UB.2 does not change temperature or microcirculation at the acupoint UB.67 in healthy volunteers under constant experimental conditions.