Increased tissue factor pathway inhibitor in patients with acute myocardial infarction

Abstract

We examined hemostatic abnormalities in 23 patients with acute myocardial infarction (AMI), 10 with pulmonary embolism (PE), and 10 with deep vein thrombosis (DVT). At the onset of AMI, plasma levels of tissue-type plasminogen activator (t-PA), PA inhibitor-I (PAI-I), fibrin-D-dimer, thrombin-antithrombin complex (TAT), and plasmin-plasmin inhibitor complex (PPIC) were significantly increased. Both the plasma total TFPI and free-TFPI levels in the AMI patients were significantly higher than those in the healthy volunteers, PE patients, and DVT patients. There was no significant difference in total TFPI or free-TFPI among patients with PE, those with DVT, and healthy volunteers. One hour after percutaneous transluminal coronary angioplasty (PTCA) in the AMI group, the total TFPI level was further increased, and it was significantly reduced 24 hr after PTCA, to a level similar to that in healthy volunteers. Free-TFPI showed a pattern similar to that of total TFPI. The ratio of free-TFPI/total TFPI was highest 1 hr after PTCA. Increased TFPI in AMI patients might be released from ischemic tissues. Am.

Combination of a Brief Irrigation With Tissue Factor Pathway Inhibitor (TFPI) and Adenovirus-Mediated Local TFPI Gene Transfer Additively Reduces Neointima Formation in Balloon-Injured Rabbit Carotid Arteries

“…The amount of TFPI protein in the medium was measured by a 1-step sandwich ELISA method with human recombinant TFPI as a standard, as described previously. 18,24 The minimum amount of TFPI detectable by this method is 6.3 ng/mL.…”

“…The amount of TFPI protein in the medium was measured by a 1-step sandwich ELISA method with human recombinant TFPI as a standard, as described previously. 18,24 The minimum amount of TFPI detectable by this method is 6.3 ng/mL.The vessels were harvested 4 weeks later, and 4 sections (5 m thick) were cut from the middle portion of each vessel. The cross-sectional areas of neointima and media were measured morphometrically with an automated computer-based image analyzer (DKC-5000, SONY) by a technician blinded to the treatment regimen.…”

Circulating thrombotic and haemostatic components in patients with coronary artery disease

“…The secreted TFPI from monocytes and most vascular wall cells may be associated within plaque through the binding with proteoglycans and other TFPI binding protein on the cells (30,31). It has been reported in literature to be elevated in AMI patients (5,(7)(8)(9). Our results indicate increase levels in younger AMI patients while insignificant changes in the levels of plasma TFPI in elder AMI patients as compared to controls.…”

“…As synthesis and secretion of TFPI is enhanced by heparin (32,33), it may have caused increase TFPI in Group 2 patients on treatment. Literature reports elevated levels in unstable angina patients (5,(34)(35)(36)(37) and marginal increase in case of stable angina patients (7). Moreover in our study we observed significant decrease in TFPI in AMI patients who had added risk factor of smoking.…”

In Search for Novel Biomarkers of Acute Coronary Syndrome

“…It is also known to activate factor X through intrinsic pathway by activating factor IX, leading to thrombin generation and fibrin formation. Since Suefuji et al in 1997 reported the role of TF in AMI, there have been many studies conducted to determine the status of plasma TF and AMI (Kamikura et al, 1997, Nishiyama et al, 1998, Malý et al, 2003, Morange et al, 2007, Xiong et al, 2007 with contradictory findings. We observed increased levels of TF in AMI at presentation (Shalia et al, 2010a).…”