Increased transcription of mitochondrial genes for Complex I in human platelets during ageing

Pich, Milena Merlo; Raule, Nicola; Catani, Lucia; Fagioli, Matteo; Faenza, Irene; Cocco, Lucio; Lenaz, Giorgio

doi:10.1016/s0014-5793(03)01520-5

Cited by 21 publications

(2 citation statements)

References 26 publications

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“…The role of mitochondria in aging is well known and majorly seen in declining mitochondrial function and accumulation of mtDNA deletions ( 17 – 19 ). Increased levels of the mitochondrial Complex I and IV genes COX I, ND1, and ND5 were observed in platelets isolated from adult healthy subjects (69–82 years) when compared to levels in young healthy subjects (21–29 years) ( 20 ), supporting our findings. One could argue that this finding was subject to potential detection of DNA as a result of different blood collection methodologies.…”

Section: Discussionsupporting

confidence: 90%

Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection

et al. 2017

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Despite potent immunosuppression, clinical and biopsy confirmed acute renal allograft rejection (AR) still occurs in 10–15% of recipients, ~30% of patients demonstrate subclinical rejection on biopsy, and ~50% of them can show molecular inflammation, all which increase the risk of chronic dysfunction and worsened allograft outcomes. Mitochondria represent intracellular endogenous triggers of inflammation, which can regulate immune cell differentiation, and expansion and cause antigen-independent graft injury, potentially enhancing the development of acute rejection. In the present study, we investigated the role of mitochondrial DNA encoded gene expression in biopsy matched peripheral blood (PB) samples from kidney transplant recipients. Quantitative PCR was performed in 155 PB samples from 115 unique pediatric (<21 years) and adult (>21 years) renal allograft recipients at the point of AR (n = 61) and absence of rejection (n = 94) for the expression of 11 mitochondrial DNA encoded genes. We observed increased expression of all genes in adult recipients compared to pediatric recipients; separate analyses in both cohorts demonstrated increased expression during rejection, which also differentiated borderline rejection and showed an increasing pattern in serially collected samples (0–3 months prior to and post rejection). Our results provide new insights on the role of mitochondria during rejection and potentially indicate mitochondria as targets for novel immunosuppression.

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Section: Discussionsupporting

confidence: 90%

Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection

et al. 2017

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“…Based on our results, we propose the use of the mitochondrial isoform of dUTPase as a potential reference gene for studies involving comparisons between different organs in mice. For gene expression studies involving mitochondrial proteins, normalization is usually carried out to expression levels of traditional reference genes ; however, the use of transcripts for mitochondrial proteins as reference genes, such as the mitochondrial isoform of dUTPase, may be beneficial for gene expression studies of mitochondrial proteins as well.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of critical design parameters for RT‐qPCR‐based analysis of multiple dUTPase isoform genes in mice

et al. 2019

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The coupling of nucleotide biosynthesis and genome integrity plays an important role in ensuring faithful maintenance and transmission of genetic information. The enzyme dUTPase is a prime example of such coupling, as it generates dUMP for thymidylate biosynthesis and removes dUTP for synthesis of uracil‐free DNA. Despite its significant role, the expression patterns of dUTPase isoforms in animals have not yet been described. Here, we developed a detailed optimization procedure for RT‐qPCR‐based isoform‐specific analysis of dUTPase expression levels in various organs of adult mice. Primer design, optimal annealing temperature, and primer concentrations were specified for both nuclear and mitochondrial dUTPase isoforms, as well as two commonly used reference genes, GAPDH and PPIA. The linear range of the RNA concentration for the reverse transcription reaction was determined. The PCR efficiencies were calculated using serial dilutions of cDNA. Our data indicate that organs involved in lymphocyte production, as well as reproductive organs, are characterized by high levels of expression of the nuclear dUTPase isoform. On the other hand, we observed that expression of the mitochondrial dUTPase isoform is considerably increased in heart, kidney, and ovary. Despite the differences in expression levels among the various organs, we also found that the mitochondrial dUTPase isoform shows a much more uniform expression pattern as compared to the reference genes GAPDH and PPIA.

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Oxidative Stress and Leaf Senescence

Zentgraf

2018

Annual Plant Reviews Online

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Increased transcription of mitochondrial genes for Complex I in human platelets during ageing

Cited by 21 publications

References 26 publications

Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection

Expression of Mitochondrial-Encoded Genes in Blood Differentiate Acute Renal Allograft Rejection

Evaluation of critical design parameters for RT‐qPCR‐based analysis of multiple dUTPase isoform genes in mice

Oxidative Stress and Leaf Senescence

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