Increased weight loss with reduced viral replication in interleukin-10 knock-out mice infected with murine cytomegalovirus

Oakley, Oliver R.; Garvy, Beth A.; Humphreys, Stewart R.; Qureshi, Mahboob; Pomeroy, Claire

doi:10.1111/j.1365-2249.2007.03533.x

Cited by 34 publications

(48 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Interestingly, in vivo blocking of IL-10 signaling during MCMV infection increased numbers of CD4 T cells which could be triggered to produce IFN-␥ after unspecific TCR stimulation. This was associated with reduced virus titers in the salivary glands but also with increased immunopathology (27,42).…”

Section: Discussionmentioning

confidence: 99%

The Dynamics of Mouse Cytomegalovirus-Specific CD4 T Cell Responses during Acute and Latent Infection

Walton

Wyrsch

Munks

et al. 2008

The Journal of Immunology

View full text Add to dashboard Cite

The dynamics of mouse cytomegalovirus (MCMV)-specific CD4 T cell responses and the mechanisms by which these cells contribute to viral control are not well understood, mainly due to lack of appropriate tools to characterize MCMV-specific CD4 T cells. We therefore generated MCMV-specific CD4 T cell hybridomas, then used an MCMV expression library and overlapping peptides to identify CD4 T cell epitopes. We used these novel tools to study the long-term kinetics and organ distribution of MCMV-specific CD4 T cells in comparison to MCMV-specific CD8 T cell responses. We demonstrate that the overall MCMV-specific CD4 T cell response stabilizes during the latent stage, which stands in contrast to subpopulations of MCMV-specific CD8 T cells and HCMV-specific CD4 T cells which accumulate over the course of CMV latency. Furthermore, MCMV-specific CD4 T cells displayed a Th1 phenotype, secreting high levels of IFN-γ and TNF-α and to some extent IL-2, cytokines which are involved in protection from CMV disease.

show abstract

Section: Discussionmentioning

confidence: 99%

The Dynamics of Mouse Cytomegalovirus-Specific CD4 T Cell Responses during Acute and Latent Infection

Walton

Wyrsch

Munks

et al. 2008

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…It is important to note that cytomegalovirus has a huge genome which enables it to express various proteins, inducing a complicated immune process. The antiviral mechanisms of spleens are also complex, and which approach is employed to resist infection has not yet been confirmed [9, 29]. Through the model of MCMV-infected mice, we focused on the antiviral mechanism of spleens in BALB/c and C57BL/6 mice, trying to learn more about the different susceptibilities to MCMV in the two mouse strains.…”

Section: Discussionmentioning

confidence: 99%

“…In this way, IFNγ actually helps to overcome viral CD8 T cell immune evasion [38]. NK cells might be the source of IFNγ during the first 3 days postinfection and T cells expressed IFNγ at a later stage [9]. IL18 is a crucial immune factor which facilitates secretion of IFNγ to control virus infections [22, 39].…”

Section: Discussionmentioning

confidence: 99%

“…The spleen is an important lymphoid organ containing various immune cells and cytokines [9, 13]. As members of them, NK cells and CD8 T cells are important in the resistance of MCMV [30].…”

Section: Discussionmentioning

confidence: 99%

“…The spleen is a downstream central lymphoid organ and a common site of MCMV infection, which adopts various immune strategies to resist infection [9, 10]. The spleen contains abundant immune cells, such as NK cells, dendritic cells (DCs), and T cells, and except for the Ly49H-m157 pathway of NK cells, other factors are very likely involved in the antiviral responses [11-13].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Effects of IL10 and NK Cells on the Susceptibility to Mouse Cytomegalovirus in BALB/c Mice despite the Compensation of IFNγ

Lu¹,

Liu²,

Huang³

et al. 2018

Intervirology

View full text Add to dashboard Cite

Objective: The aim of this study was to determine which factors lead to the susceptibility to mouse cytomegalovirus (MCMV) in the spleens of BALB/c mice. Methods: BALB/c and C57BL/6 mice were randomly divided into a control group and an infection group and sacrificed on day 0, 1, 3, 7, 14, and 28 postinfection. The cytotoxicity of NK cells was determined by evaluating lactate dehydrogenase contents. Flow cytometry was used to analyze activated NK cells, IFNγ⁺ NK cells, and total NK cells in the spleen. The pathological changes of spleens in each group were analyzed by HE staining. The expression of IL10, IL18, IFNγ, Thpok, and IFNβ of spleens was determined by quantitative reverse transcriptase PCR. The viral loads of MCMV in spleens and salivary glands were also detected. Results: We found that spleen NK cells and IL10 in C57BL/6 mice possessed more powerful immunity to MCMV than BALB/c mice. In BALB/c mice, combined effects of the cytotoxicity of NK cells and IFNγ in spleens still ended up with deficient control of infection. Conclusion: The functional shortage of NK cells and inappropriate expression of IL10 result in the susceptibility to MCMV in BALB/c mice.

show abstract

Epidemiological characteristics and cerebrospinal fluid cytokine profiles of enterovirus encephalitis in children in Hangzhou, China

Sun

et al. 2021

Journal of Medical Virology

View full text Add to dashboard Cite

Enteroviruses (EVs) are common causes of viral encephalitis in children. To better understand the epidemiological and pathological characteristics of EV encephalitis, we enrolled suspected encephalitis patients younger than 15 years old in Hangzhou, China, from October 2016 to September 2019 for cerebrospinal fluid (CSF) collection and analyses. A total of 7735 CSF samples were collected, among which 330 (4.27%) were positive for the EV genome. The positivity rate was significantly higher in boys than girls (χ 2 = 5.68, p = 0.02).The monthly case numbers peaked from June to August (80.30%). Among the different age groups, the 0-2 months age group showed the highest number of cases (28.48% of all cases). The 6-7 years (10.82%) and 9-10 years (9.29%) age groups showed the highest EV-positivity rates among suspected encephalitis cases. Sixty-two EV-positive and 53 control CSF samples were collected for Bio-Plex Pro human cytokine assays that simultaneously tested 48 cytokines.Principle component analyses showed significant separation between EV-positive and control samples, but insignificant separation between children and newborns. The levels of 28 cytokines and chemokines were significantly elevated in the EV-positive group including many proinflammatory and a few anti-inflammatory cytokines, as well as chemokines belonging to the CC and CXC subfamilies. Only one cytokine, stem cell growth factor-β, showed a decrease in the EV-positive group. Thus, this study revealed age, sex, and seasonal preferences for EV encephalitis incidences in children and identified many cytokines dysregulated during EV encephalitis.

show abstract

Increased weight loss with reduced viral replication in interleukin-10 knock-out mice infected with murine cytomegalovirus

Cited by 34 publications

References 43 publications

The Dynamics of Mouse Cytomegalovirus-Specific CD4 T Cell Responses during Acute and Latent Infection

The Dynamics of Mouse Cytomegalovirus-Specific CD4 T Cell Responses during Acute and Latent Infection

The Effects of IL10 and NK Cells on the Susceptibility to Mouse Cytomegalovirus in BALB/c Mice despite the Compensation of IFNγ

Epidemiological characteristics and cerebrospinal fluid cytokine profiles of enterovirus encephalitis in children in Hangzhou, China

Contact Info

Product

Resources

About