Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

Rysz, Jacek; Potargowicz, Elż̇bieta; Banach, Maciej; Luczyńska, Maria; Stolarek, Robert; Białasiewicz, Piotr; Kasielski, Marek; Ciałkowska-Rysz, Aleksandra; Nowak, Dariusz

doi:10.1007/s00005-006-0040-0

Cited by 13 publications

(15 citation statements)

References 47 publications

(77 reference statements)

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“…rLBCL correlated positively with the number of circulating PMNs; thus, it may reflect baseline oxidant production by blood phagocytes. rLBCL was elevated in various diseases displaying a strong inflammatory response, including infective endocarditis [19], systemic sclerosis [29], and chronic renal insufficiency [30]. It increased substantially after cardiac valve replacement surgery…”

Section: Resting Luminol-enhanced Whole-blood Chemiluminescencementioning

confidence: 99%

Addition of Strawberries to the Usual Diet Decreases Resting Chemiluminescence of Fasting Blood in Healthy Subjects—Possible Health-Promoting Effect of These Fruits Consumption

Białasiewicz

Prymont-Przymińska

Zwolinska

et al. 2014

Journal of the American College of Nutrition

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Section: Resting Luminol-enhanced Whole-blood Chemiluminescencementioning

confidence: 99%

Addition of Strawberries to the Usual Diet Decreases Resting Chemiluminescence of Fasting Blood in Healthy Subjects—Possible Health-Promoting Effect of These Fruits Consumption

Białasiewicz

Prymont-Przymińska

Zwolinska

et al. 2014

Journal of the American College of Nutrition

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“…37 During HD sessions, a direct increase in blood levels of ROS has been demonstrated using whole blood chemiluminescence. 38 ROS has been incriminated in the lipid peroxidation, atherogenesis, and increased risk of CVD in patients with uremia and considered to act by increasing the activity of monocytes and oxidation of LDL. 39,40 The latter modification is considered to play a pivotal role in the initiation and progression of atherosclerosis because the oxidized LDL stimulates foam cell formation.…”

Section: Discussionmentioning

confidence: 99%

Paraoxonase Enzyme Activity Is Enhanced by Zinc Supplementation in Hemodialysis Patients

et al. 2012

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Background and Aims: Patients on maintenance hemodialysis (HD) face an increased risk of atherosclerosis, a crucial problem and the leading cause of cardiovascular morbidity and mortality. This study was designed to evaluate the effects of zinc supplementation on paraoxonase (PON) enzyme activity in patients on HD. Methods: This double-blind randomized controlled trial was conducted from June 2005 to June 2007. Sixty HD patients were enrolled and divided into two groups: treatment (case) and control. The treatment and control groups were treated with 100 mg/day zinc or placebo, respectively, for 2 months. Serum zinc concentration was measured by atomic absorption spectrophotometry. PON activity was evaluated by spectrophotometric method. Lipid profile was determined using commercial kits, and apolipoprotein AI (Apo-AI) and B (Apo-B) levels were measured by commercial immunoturbidimetric kits. Results: In the case group, there was no significant change in the serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), and Apo-B levels, while the serum levels of high-density lipoprotein (HDL), Apo-AI, and PON activity were significantly increased (p ¼ 0.02). In the control group, although significant increases were observed in the serum levels of TC, TG, and Apo-B (p ¼ 0.009, 0.019, and 0.001, respectively), the serum PON activity was significantly decreased (p ¼ 0.025) and the serum levels of HDL, LDL, and Apo-AI were not changed. At the end of intervention period, the serum level of Apo-AI and PON activity were significantly higher in the case group. Conclusions: Zinc supplementation increased both the activity of PON and the serum level of Apo-AI in the HD patients.

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“…The high frequency of bacterial infections in patients with chronic renal disease suggests that their ability to generate ROS and to kill bacteria may by impaired by uremia. Conflicting results demonstrating increase [40], lack of relevant changes [41] or inhibition of PMN respiratory burst [31] suggests that intensity of ROS production in uremic patients is the result of the influence of huge number of diverse uremic toxins on PMN activity. There is only 1 paper, by Leung-Tack et al, describing the inhibitory effect of cC on PMN respiratory burst in response to opsonized zymosan [42].…”

Section: Discussionmentioning

confidence: 99%

The influence of uremic high cystatin C concentration on neutrophil apoptosis and selected neutrophil functions isolated from healthy subjects

et al. 2012

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SummaryBackgroundCystatin C (cC) is a cysteine protease inhibitor that may influence immune response. Our aim was to test the effect of a high concentration of cC, characteristic for uremic patients, on neutrophil (PMN) apoptosis and respiratory burst, as well as the cC secretion from PMNs stimulated with proinflammatory cytokines.Material/MethodsPMNs from 35 healthy volunteers aged 27–61 years were cultured in presence of cC, IL-1β or TNF-α. The percentage of apoptotic cells based on DNA depletion, Fas, FasL and caspase -3 expression were assessed. CC concentrations were determined by ELISA test. The influence of cC on spontaneous, fMLP-, PMA- or OZ-induced burst response of PMNs was tested using chemiluminescence.ResultsPMN cultured in the presence of cC resulted in a significant drop in apoptotic cell percentage (38% [11%; 65%]) compared both to control (70% [29%; 92%], and to the cells cultured with TNF-α (58% [24%; 85%]). These differences were not accompanied by Fas, FasL and caspase-3 expression changes. Spontaneous, fMLP- and PMA-stimulated oxidative burst of PMNs preincubated with cC were significantly downregulated. IL-1β markedly diminished and TNF-α significantly increased cC concentration in culture supernatants.ConclusionsThe presented results suggest that antiapoptotic activity of cC results from its inhibitory effect on ROS production. Thus, the higher concentration of cC characteristic for uremic patients may modulate acute inflammation through maintaining PMN longevity and inhibiting their respiratory burst and proinflammatory cytokine-related changes in cC release from PMNs.

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Increased whole blood chemiluminescence in patients with chronic renal failure independent of hemodialysis treatment

Abstract: Blood from patients with renal failure generates elevated amounts of oxidants independently of HD treatment. This may add to the understanding of the nature of oxidative stress and suggests the need of anti-oxidant treatment in these patients.

Cited by 13 publications

References 47 publications

Addition of Strawberries to the Usual Diet Decreases Resting Chemiluminescence of Fasting Blood in Healthy Subjects—Possible Health-Promoting Effect of These Fruits Consumption

Addition of Strawberries to the Usual Diet Decreases Resting Chemiluminescence of Fasting Blood in Healthy Subjects—Possible Health-Promoting Effect of These Fruits Consumption

Paraoxonase Enzyme Activity Is Enhanced by Zinc Supplementation in Hemodialysis Patients

The influence of uremic high cystatin C concentration on neutrophil apoptosis and selected neutrophil functions isolated from healthy subjects

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