Increases in Circulating Cell-Free DNA During Aerobic Running Depend on Intensity and Duration

Haller, Nils; Tug, Suzan; Breitbach, Sarah; Jörgensen, Arne; Simon, Perikles

doi:10.1123/ijspp.2015-0540

Cited by 45 publications

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“…CfDNA levels are known to increase during exercise and up to 8-fold rises have been reported following moderate aerobic running. 10 In agreement with our primary analysis, a vast number of analytes have been demonstrated to display comparable biological variation in healthy and diseased subjects. 19 This supports the applicability of cfDNA CVs and RCVs estimated from healthy subjects to cancer patients and possibly patients with other chronic illnesses as well.…”

Section: Discussionsupporting

confidence: 83%

Day-to-day and within-day biological variation of cell-free DNA

et al. 2019

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Background: Numerous studies have shown that cell-free DNA (cfDNA) levels may serve as a non-invasive biomarker of a broad spectrum of acute and chronic pathologies. However, in order to make clinical decisions based on cfDNA measurements, it is essential to understand the magnitude of biological variation so this variation is not confused with a variation that actually represent a clinically relevant change. The present study was designed to evaluate the biological variation of cfDNA in healthy subjects and lung cancer patients. Methods: Plasma samples were collected from 33 healthy subjects and ten lung cancer patients over three days, as well as during the same day. CfDNA was quantified using droplet digital PCR. Biological variation data was estimated using mixed models. Findings: The within-subject variation was 25% and the between-subject variation was 30%. The reference change value for the healthy subjects was 70%. There was no systematic difference in cfDNA levels from day-today (p = 0 61), but there was a significant decline during the day (p < 0 01). The within-subject variation in cancer patients was comparable to healthy subjects, whereas the between-subject variation was much larger (139%). No systematic differences from day-today were observed for the cancer patients (p > 0 3). Interpretation: Our findings show that cfDNA levels fluctuate significantly during the day and exhibit considerable within-subject variation. Thus, the data presented offer a substantial contribution to the interpretation of the clinical significance of cfDNA. Funding: Laege Sofus Carl Emil Friis og hustru Olga Doris Friis' Legat, Harboefonden, and Dagmar Marshalls Fond.

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Section: Discussionsupporting

confidence: 83%

Day-to-day and within-day biological variation of cell-free DNA

et al. 2019

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“…Thus, we assume that our cortisol dataset based on the measurement procedure with three time points (08:30 AM, 10:30 AM, and 12:30 PM) is not entirely comparable with the time points used previously [45]. Some studies have reported that cfDNA levels already increase with moderate PA below the level of the aerobic-anaerobic transition [26,28]. The results of the cfDNA AUCi posterior distributions suggest that such a association is also more likely in both teaching settings.…”

Section: A) B) C) D)mentioning

confidence: 94%

“…Based on plasma samples, the cfDNA is a well-established indicator of the activation of the innate immunity. Various studies have revealed that the innate immunity could be activated by both psychologically [21][22] and physiologically [23][24][25][26][27] stressful situations. In particular, the cfDNA has been proven to be highly sensitive to physical exercise as a stressor (see [27] for review).…”

Section: Introductionmentioning

confidence: 99%

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Children’s Cortisol and Cell-Free DNA Trajectories in Relation to Sedentary Behavior and Physical Activity in School: A Longitudinal Analysis

Becker¹,

Schmidt²,

Neuberger³

et al. 2018

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Education outside the classroom (EOtC) can be beneficial for students. The relationship between biological stress markers and sedentary behavior (SB) plus physical activity (PA) is insufficiently evaluated in school settings. This exploratory study aims to evaluate the association between students’ cortisol, plus circulating cell-free deoxyribonucleic acid (cfDNA) levels, and their SB, light PA (LPA) and moderate-to-vigorous PA (MVPA) during outdoor and indoor classes in different seasons. We assessed data from an education outside the classroom (EOtC) program (n = 48; intervention group [IG], n = 37; control group [CG], n = 11). We sampled data on 3 school-days in three seasons (fall, spring, and summer) in normal teaching indoors (CG) and outdoor lessons (IG) in the forest. SB and PA were evaluated by accelerometry, and cortisol and cfDNA levels by saliva samples. The compositional data analysis approach analyzed SB and PA. Fitted Bayesian hierarchical linear models evaluated the association between cortisol and cfDNA, and SB/LPA/MVPA. A steady decline of cortisol in the outdoor setting is associated with relatively high levels of LPA. SB and MVPA tended to exhibit a similar effect in the indoor setting. CfDNA is positively associated with a relatively high amount of SB in the IG, the same association is likely for LPA and MVPA in both groups. LPA seems to support a healthy cortisol decrease in children during outdoor lessons. The relevance of SB/PA as a composition in relation to students stress response in school should be emphasized. This study facilitates the formulation of straightforward and directed hypotheses for further research.

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“…While the mechanism underpinning the increased release of cfDNA during exercise is poorly understood, it appears that cfDNA is primarily released from cells involved in immune function [119]. The magnitude of cfDNA concentration increases also appears proportional to both exercise intensity and duration [119,120], and the time course of these changes is remarkably transient, with cfDNA concentrations often returning to baseline within 24 h, even after highly exhaustive exercise [115]. As a result, cfDNA represents a potentially novel biomarker for fatigue and recovery in exercise [113,119,120].…”

Section: Section Summarymentioning

confidence: 99%

The Development of a Personalised Training Framework: Implementation of Emerging Technologies for Performance

Pickering

Kiely

2019

JFMK

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Over the last decade, there has been considerable interest in the individualisation of athlete training, including the use of genetic information, alongside more advanced data capture and analysis techniques. Here, we explore the evidence for, and practical use of, a number of these emerging technologies, including the measurement and quantification of epigenetic changes, microbiome analysis and the use of cell-free DNA, along with data mining and machine learning. In doing so, we develop a theoretical model for the use of these technologies in an elite sport setting, allowing the coach to better answer six key questions: (1) To what training will my athlete best respond? (2) How well is my athlete adapting to training? (3) When should I change the training stimulus (i.e., has the athlete reached their adaptive ceiling for this training modality)? (4) How long will it take for a certain adaptation to occur? (5) How well is my athlete tolerating the current training load? (6) What load can my athlete handle today? Special consideration is given to whether such an individualised training framework will outperform current methods as well as the challenges in implementing this approach.

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Increases in Circulating Cell-Free DNA During Aerobic Running Depend on Intensity and Duration

Cited by 45 publications

References 0 publications

Day-to-day and within-day biological variation of cell-free DNA

Day-to-day and within-day biological variation of cell-free DNA

Children’s Cortisol and Cell-Free DNA Trajectories in Relation to Sedentary Behavior and Physical Activity in School: A Longitudinal Analysis

The Development of a Personalised Training Framework: Implementation of Emerging Technologies for Performance

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Increases in Circulating Cell-Free DNA During Aerobic Running Depend on Intensity and Duration

Cited by 45 publications

References 0 publications

Day-to-day and within-day biological variation of cell-free DNA

Day-to-day and within-day biological variation of cell-free DNA

Children&rsquo;s Cortisol and Cell-Free DNA Trajectories in Relation to Sedentary Behavior and Physical Activity in School: A Longitudinal Analysis

The Development of a Personalised Training Framework: Implementation of Emerging Technologies for Performance

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Product

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Children’s Cortisol and Cell-Free DNA Trajectories in Relation to Sedentary Behavior and Physical Activity in School: A Longitudinal Analysis