Increasing disease resistance in host plants through genome editing

Karmakar, Aritra; Taufiqa, Syeda; Baig, M. J.; Molla, Kutubuddin A.

doi:10.1007/s43538-022-00100-6

Cited by 11 publications

(6 citation statements)

References 80 publications

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“…It allows researchers to target specific genes involved in nematode resistance, making the process more precise and efficient. Additionally, gene editing can accelerate the development of new crop varieties, as traditional methods may take years to breed and test crops for resistance [ 42 , 75 , [80] , [81] , [82] , [83] , [84] ].…”

Section: Deploying Resistant Crop Varieties: An Effective and Sustain...mentioning

confidence: 99%

Revolutionizing nematode management to achieve global food security goals - An overview

Afzal,

Mukhtar

2024

Heliyon

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Section: Deploying Resistant Crop Varieties: An Effective and Sustain...mentioning

confidence: 99%

Revolutionizing nematode management to achieve global food security goals - An overview

Afzal,

Mukhtar

2024

Heliyon

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“…Modern genome editing (GE) technologies have been instrumental in crop improvement, especially in terms of boosting disease resistance. The CRISPR/Cas9 system, CRISPR/Cas13, base editing, transcription activator-like effector nucleases (TALENs), zinc-finger nucleases (ZFNs), and meganucleases are some of these cutting-edge techniques which have been utilized from crop improvement ( Karmakar et al, 2022 ). With the use of these versatile tools, crop genomes may be precisely and specifically modified, opening up the enormous potential to engineer disease resistance in crops by changing certain genes involved in the plant immune system.…”

Section: Types Of Genome Editing Techniquesmentioning

confidence: 99%

“…However, genetic disruption of these genes has proven to be one of the most effective methods for mitigating plant diseases and inducing long-term disease resistance. Interestingly, novel genome-editing methods provide prospects for controlling bacterial, viral, and fungal infections, as well as implementing pathogen resistance in plants ( Karmakar et al, 2022 ). Among genome editing tools, CRISPR/Cas9 has been regarded as a successful technique due to its versatility, lower cost, ease of design and implementation, and increased success rate ( Saraswat et al, 2024 ).…”

Section: Genome Editing Enhances Host Plant Resistancementioning

confidence: 99%

Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production

Manzoor,

Nabi,

Rather

et al. 2024

Front. Genome Ed.

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Modern agriculture has encountered several challenges in achieving constant yield stability especially due to disease outbreaks and lack of long-term disease-resistant crop cultivars. In the past, disease outbreaks in economically important crops had a major impact on food security and the economy. On the other hand climate-driven emergence of new pathovars or changes in their host specificity further poses a serious threat to sustainable agriculture. At present, chemical-based control strategies are frequently used to control microbial pathogens and pests, but they have detrimental impact on the environment and also resulted in the development of resistant phyto-pathogens. As a replacement, cultivating engineered disease-resistant crops can help to minimize the negative impact of regular pesticides on agriculture and the environment. Although traditional breeding and genetic engineering have been instrumental in crop disease improvement but they have certain limitations such as labour intensity, time consumption, and low efficiency. In this regard, genome editing has emerged as one of the potential tools for improving disease resistance in crops by targeting multiple traits with more accuracy and efficiency. For instance, genome editing techniques, such as CRISPR/Cas9, CRISPR/Cas13, base editing, TALENs, ZFNs, and meganucleases, have proved successful in improving disease resistance in crops through targeted mutagenesis, gene knockouts, knockdowns, modifications, and activation of target genes. CRISPR/Cas9 is unique among these techniques because of its remarkable efficacy, low risk of off-target repercussions, and ease of use. Some primary targets for developing CRISPR-mediated disease-resistant crops are host-susceptibility genes (the S gene method), resistance genes (R genes) and pathogen genetic material that prevents their development, broad-spectrum disease resistance. The use of genome editing methods has the potential to notably ameliorate crop disease resistance and transform agricultural practices in the future. This review highlights the impact of phyto-pathogens on agricultural productivity. Next, we discussed the tools for improving disease resistance while focusing on genome editing. We provided an update on the accomplishments of genome editing, and its potential to improve crop disease resistance against bacterial, fungal and viral pathogens in different crop systems. Finally, we highlighted the future challenges of genome editing in different crop systems for enhancing disease resistance.

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“…The advent of CRISPR/Cas9 has opened numerous possibilities for developing improved crop varieties through precise and accurate genome editing. CRISPR techniques have ushered in a new era of breeding systems, where plant immunity can be enhanced by disrupting the compatible interaction between pathogens and hosts . CRISPR-based tools such as SHERLOCK, FLASH, and LEOPARD, which utilize Cas12 and Cas13 proteins, have proven useful for disease identification, diagnosis, and management in sugar beet.…”

Section: Utilizing Crispr Tools For Disease Diagnosis In Sugar Beetmentioning

confidence: 99%

Exploring CRISPR/Cas9 Gene Editing Applications for Enhancing Disease Resistance in Sugar Beet

Misra,

Pandey,

Mall

2023

ACS Agric. Sci. Technol.

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Sugar beet, an important sugar crop utilized for the production of beet sugar, fulfills the world's sugar demands to about 25%, accompanying cane sugar. The production of sugar beet encounters significant challenges attributed to devastating diseases that result in a decreased crop yield. Disease-resistant varieties are key to mitigating productivity losses traditionally developed through conventional breeding. However, the advent of CRISPR/Cas9 genome editing has accelerated the creation of resistant sugar beet varieties by enabling precise genetic modifications. This advanced technology offers an effective approach to combat bacterial, fungal, and viral pathogens, leading to the development of sugar beet varieties with varying degrees of resistance. The paper elucidates the revolutionary impact of CRISPR/Cas9 on sugar beet, revealing essential regulators of disease resistance and providing insights into interactions with pathogens and the sugar beet microbiome. The integration of CRISPR/Cas9 with other techniques opens novel avenues for understanding and developing disease-resistant sugar beet varieties, marking a significant advancement in the field.

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Increasing disease resistance in host plants through genome editing

Cited by 11 publications

References 80 publications

Revolutionizing nematode management to achieve global food security goals - An overview

Revolutionizing nematode management to achieve global food security goals - An overview

Advancing crop disease resistance through genome editing: a promising approach for enhancing agricultural production

Exploring CRISPR/Cas9 Gene Editing Applications for Enhancing Disease Resistance in Sugar Beet

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