Increasing glucose in KSOMaa basal medium on culture Day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer

Kwong, Phek Jin; Abdullah, Ramli; Khadijah, W. E. Wan

doi:10.1016/j.theriogenology.2012.04.009

Cited by 7 publications

(10 citation statements)

References 47 publications

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“…; Kwong et al. ; Soh et al. ), the IVD competence of cloned caprine embryos improved using MSCs as donor karyoplasts.…”

Section: Discussionmentioning

confidence: 99%

“…This protocol was performed as that reported by Kwong et al. (). Incubation was performed under a humidified atmosphere with 5% CO 2 in air at 38.5°C.…”

Section: Methodsmentioning

confidence: 99%

“…In vitro culture of reconstructed oocytes Reconstructed oocytes were cultured in KSOMaa with glucose (0.2 mM) for 2 days (2D) followed by KSOMaa with 2.78 mM glucose (final concentration) for another 6 days (6D). This protocol was performed as that reported by Kwong et al (2012). Incubation was performed under a humidified atmosphere with 5% CO 2 in air at 38.5°C.…”

Section: Activation Of Reconstructed Oocytesmentioning

confidence: 99%

“…One of the key factors is the nuclear donor cell type. For caprine NT, which is a popular model, many somatic cell types, including those from mammary epithelia (Zhang et al 2008;Yuan et al 2009), ovarian cumulus (Zou et al 2001;Keefer et al 2002), foetal fibroblasts (Baguisi et al 1999;Keefer et al 2002;Melican et al 2005;Soh et al 2012) and skin (Wang et al 2007;Abdullah et al 2011;Kwong et al 2012;Wan et al 2012), have been utilized. A clear consensus has not yet been reached as to which somatic cell type is best for nuclear transfer.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of In Vitro Developmental Competence of Cloned Caprine Embryos Using Donor Karyoplasts from Adult Bone Marrow Mesenchymal Stem Cells vs Ear Fibroblast Cells

Kwong

Nam

Khadijah

et al. 2014

Reprod Domestic Animals

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The aim of this study was to produce cloned caprine embryos using either caprine bone marrow-derived mesenchymal stem cells (MSCs) or ear fibroblast cells (EFCs) as donor karyoplasts. Caprine MSCs were isolated from male Boer goats of an average age of 1.5 years. To determine the pluripotency of MSCs, the cells were induced to differentiate into osteocytes, chondrocytes and adipocytes. Subsequently, MSCs were characterized through cell surface antigen profiles using specific markers, prior to their use as donor karyoplasts for nuclear transfer. No significant difference (p > 0.05) in fusion rates was observed between MSCs (87.7%) and EFCs (91.3%) used as donor karyoplasts. The cleavage rate of cloned embryos derived with MSCs (87.0%) was similar (p > 0.05) to those cloned using EFCs (84.4%). However, the in vitro development of MSCs-derived cloned embryos (25.3%) to the blastocyst stage was significantly higher (p < 0.05) than those derived with EFCs (20.6%). In conclusion, MSCs could be reprogrammed by caprine oocytes, and production of cloned caprine embryos with MSCs improved their in vitro developmental competence, but not in their fusion and cleavage rate as compared to cloning using somatic cells such as EFCs.

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“…; Kwong et al. ; Soh et al. ), the IVD competence of cloned caprine embryos improved using MSCs as donor karyoplasts.…”

Section: Discussionmentioning

confidence: 99%

“…This protocol was performed as that reported by Kwong et al. (). Incubation was performed under a humidified atmosphere with 5% CO 2 in air at 38.5°C.…”

Section: Methodsmentioning

confidence: 99%

Section: Activation Of Reconstructed Oocytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Comparison of In Vitro Developmental Competence of Cloned Caprine Embryos Using Donor Karyoplasts from Adult Bone Marrow Mesenchymal Stem Cells vs Ear Fibroblast Cells

Kwong

Nam

Khadijah

et al. 2014

Reprod Domestic Animals

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“…In goats, several reports have demonstrated that factors such α-lipoic acid, activin-A and retinoids enhance embryonic development when supplemented during oocyte maturation and/or embryo culture (Bormann et al, 2003;Chiamenti et al, 2010;Kwong et al, 2012;Zhang et al, 2013;Hammami et al, 2014). Vitamin A and its physiological metabolites, collectively known as retinoids, play important roles in embryonic morphogenesis and reproductive physiology as a mitogenic and differentiation stimulus (Hofmann and Eichele, 1994).…”

mentioning

confidence: 99%

Use of retinoids during oocyte maturation diminishes apoptosis in caprine embryos

Conceição

Moura

Ferreira-Silva

et al. 2015

Acta Veterinaria Hungarica

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Exposure of caprine oocytes and embryos to retinoids enhances embryonic development, but the mechanisms governing this phenomenon have not been characterised. The aim of the present study was to evaluate if the incidence of apoptosis is affected by the addition of retinyl acetate (RAc) and 9-cis-retinoic acid (RA) during in vitro maturation (IVM) of caprine oocytes. Embryonic development was recorded on days 3 and 8 post-fertilisation, and apoptosis was measured by caspase activity and DNA fragmentation (TUNEL assay). Control zygotes had lower capacity to cleave and reach the blastocyst stage (24.45 ± 2.32 and 5.32 ± 0.81, respectively) than those of RAc-(29.96 ± 1.62 and 7.94 ± 0.93, respectively) and RA-treated groups (30.12 ± 1.51 and 7.36 ± 1.02, respectively). Oocytes and blastocysts positive for TUNEL assay were more frequent, respectively, in the controls (8.20 ± 0.78, 8.70 ± 1.05) than in RAc (5.60 ± 0.52, 4.80 ± 0.51) and RA (6.40 ± 0.69, 5.40 ± 0.69). Caspase activity did not differ between control oocytes (7.20 ± 0.91), RAc (6.60 ± 0.68) and RA (7.30 ± 0.67), but it was reduced in RAc-(5.05 ± 0.62) and RA-treated blastocysts (5.75 ± 0.22) compared to controls (8.35 ± 0.71). These results indicate that the addition of retinoids during IVM increases the developmental potential of goat embryos with a concomitant reduction in apoptosis rates.

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Interspecies Somatic Cell Nuclear Transfer

Cánovas

Cibelli

2014

Principles of Cloning

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Increasing glucose in KSOMaa basal medium on culture Day 2 improves in vitro development of cloned caprine blastocysts produced via intraspecies and interspecies somatic cell nuclear transfer

Cited by 7 publications

References 47 publications

Comparison of In Vitro Developmental Competence of Cloned Caprine Embryos Using Donor Karyoplasts from Adult Bone Marrow Mesenchymal Stem Cells vs Ear Fibroblast Cells

Comparison of In Vitro Developmental Competence of Cloned Caprine Embryos Using Donor Karyoplasts from Adult Bone Marrow Mesenchymal Stem Cells vs Ear Fibroblast Cells

Use of retinoids during oocyte maturation diminishes apoptosis in caprine embryos

Interspecies Somatic Cell Nuclear Transfer

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