Increasing heart vascularisation after myocardial infarction using brain natriuretic peptide stimulation of endothelial and WT1+ epicardial cells

Li, Na; Rignault-Clerc, Stéphanie; Bielmann, Christelle; Bon-Mathier, Anne-Charlotte; Déglise, Tamara; Carboni, Alexia; Ducrest, Mégane; Rosenblatt‐Velin, Nathalie

doi:10.7554/elife.61050

Cited by 16 publications

(43 citation statements)

References 52 publications

(43 reference statements)

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“…Epidermal cells such as keratinocytes, which are probably the sources of Nppb and dermal cells including fibroblasts and vascular endothelial cells, which expressed Npr1 and Npr3 (Figure 7G) may be related to the phenomena observed. Previous studies reported that BNP which activates cGMP‐dependent signaling had important roles in keratinocyte and endothelial cells through NPR1, 71,72 which may have roles in keratinocyte migration and angiogenesis during wound healing respectively 73,74 . This suggests a new hypothesis that BNP may contribute to wound healing by promoting keratinocyte migration and stimulation of endothelial cells for angiogenesis via a BNP/NPR1 pathway.…”

Section: Discussionmentioning

confidence: 93%

“…Previous studies reported that BNP which activates cGMP‐dependent signaling had important roles in keratinocyte and endothelial cells through NPR1, 71 , 72 which may have roles in keratinocyte migration and angiogenesis during wound healing respectively. 73 , 74 This suggests a new hypothesis that BNP may contribute to wound healing by promoting keratinocyte migration and stimulation of endothelial cells for angiogenesis via a BNP/NPR1 pathway.…”

Section: Discussionmentioning

confidence: 99%

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Longitudinal peripheral tissue RNA‐Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar surgical incision model

et al. 2021

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Section: Discussionmentioning

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Longitudinal peripheral tissue RNA‐Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar surgical incision model

et al. 2021

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“…Also, an improved migration of EPDCs into the myocardium, and a contribution to EC and SMC populations ( 130 ). Similarly, upon the injection of brain natriuretic peptide (BNP) post-MI an increase in proliferation and migration of Wt1-positive cells was observed in Wt1 CreERT2 ;Rosa26 mTmG mice, together with an increased contribution to the EC lineage ( 131 ). In both studies, the contribution to the EC population should be carefully interpreted due to native expression of Wt1 in endothelial cells after injury, which could lead to labeling of ECs in lineage-tracing experiments ( 114 ).…”

Section: The Adult Epicardiummentioning

confidence: 99%

Epicardial Contribution to the Developing and Injured Heart: Exploring the Cellular Composition of the Epicardium

Streef

Smits

2021

Front. Cardiovasc. Med.

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The epicardium is an essential cell population during cardiac development. It contributes different cell types to the developing heart through epithelial-to-mesenchymal transition (EMT) and it secretes paracrine factors that support cardiac tissue formation. In the adult heart the epicardium is a quiescent layer of cells which can be reactivated upon ischemic injury, initiating an embryonic-like response in the epicardium that contributes to post-injury repair processes. Therefore, the epicardial layer is considered an interesting target population to stimulate endogenous repair mechanisms. To date it is still not clear whether there are distinct cell populations in the epicardium that contribute to specific lineages or aid in cardiac repair, or that the epicardium functions as a whole. To address this putative heterogeneity, novel techniques such as single cell RNA sequencing (scRNA seq) are being applied. In this review, we summarize the role of the epicardium during development and after injury and provide an overview of the most recent insights into the cellular composition and diversity of the epicardium.

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“…Increased levels of ANP were also detected and could contribute to the stimulation of the CM proliferation (13).…”

Section: Discussionmentioning

confidence: 99%

Brain natriuretic peptide improves heart regeneration after infarction by stimulating cardiomyocyte renewal

Bon-Mathier

Déglise

Rignault-Clerc

et al. 2022

Preprint

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Aim: Brain Natriuretic Peptide (BNP) supplementation after infarction increases heart function and decreases heart remodeling. BNP receptors, NPR-A and NPR-B, are expressed on adult cardiomyocytes (CMs). We thus investigated whether a part of the BNP cardioprotective effect in infarcted and unmanipulated hearts is due to modulation of the CM fate. Methods: BNP was injected in infarcted adult mice and in unmanipulated neonatal and adult mice. CMs were isolated, counted and characterized. Results: Increased number of CMs was detected in the hypoxic area of infarcted hearts, and in unmanipulated neonatal and adult hearts after BNP treatment. Accordingly, Troponin T plasma concentration was significantly reduced 1 and 3 days after infarction in BNP-treated mice, demonstrating less CM death. Furthermore, higher number of small, dedifferentiated and mononucleated CMs were identified in adult BNP-treated hearts when compared to saline-treated hearts. BNP-treated CMs express higher levels of mRNAs coding for hif1 alpha and for the different cyclins than CMs isolated from saline-treated hearts. Higher percentages of CMs undergoing DNA synthesis, expressing Ki67, phospho histone3 and Aurora B were detected in all BNP-treated hearts, which suggests that BNP stimulates CMs to re-enter to the cell cycle. Results in vitro confirmed that BNP stimulates the proliferation of the neonatal CMs and the dedifferentiation of the adult CMs. BNP effect on adult CMs in vivo is mediated by NPR-A binding and activation of the ERK MAP kinase pathway. Interestingly, increased number of CMs was also detected in adult infarcted hearts treated with LCZ696, which inhibits all natriuretic peptide degradations. Conclusions: Altogether, our results identified BNP and all therapies aimed to increase the bio-availability of BNP (such as LCZ696 treatment) as new targets to increase heart regeneration. By protecting CMs from cell death, and by stimulating their proliferation, BNP treatment leads to increased number of CMs in neonatal, adult unmanipulated and infarcted hearts.

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Increasing heart vascularisation after myocardial infarction using brain natriuretic peptide stimulation of endothelial and WT1+ epicardial cells

Cited by 16 publications

References 52 publications

Longitudinal peripheral tissue RNA‐Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar surgical incision model

Longitudinal peripheral tissue RNA‐Seq transcriptomic profiling, hyperalgesia, and wound healing in the rat plantar surgical incision model

Epicardial Contribution to the Developing and Injured Heart: Exploring the Cellular Composition of the Epicardium

Brain natriuretic peptide improves heart regeneration after infarction by stimulating cardiomyocyte renewal

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