Increasing Incidence of Early Onset Colorectal Cancer in Arizona

Ohlson, Eric; DiGiovanni, Ryan; Vera, Esteban; Nfonsam, Valentine

doi:10.1016/j.jss.2013.11.214

Cited by 1 publication

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“…Colorectal cancer (CRC) is the third most common cancer in the world and tends to affect younger individuals. The morbidity of CRC in people under 50 years of age is rising rapidly [ 1 , 2 ]. Many factors affect the occurrence of CRC, such as external environment, diet, genetic factors, internal immune, and gut microbiota [ 3 , 4 ].…”

Section: Introductionmentioning

confidence: 99%

Parvimonas micra activates the Ras/ERK/c-Fos pathway by upregulating miR-218-5p to promote colorectal cancer progression

Chang

Huang

Hou

et al. 2023

J Exp Clin Cancer Res

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Background Colorectal cancer (CRC) is the third most common cancer in the world, and a strong relationship exists between CRC and gut microbiota, which affects the occurrence, development, and metastasis of cancer. Bioinformatics-based analyses revealed that the abundance of Parvimonas micra (P. micra) in the feces of patients with cancer is significantly higher than that in healthy people. Therefore, an important relationship may exist between P. micra and CRC. Methods We first confirmed that P. micra can promote the proliferation of cell lines through cell experiments and mouse models. Then we selected the signaling pathways and content of exosomes to promote the development of CRC by transcriptomics and microRNA sequencing. Finally, we confirmed that P. micra promoted CRC development through miR-218-5p/Ras/ERK/c-Fos pathway through the in vivo and in vitro experiments. Results First, it was confirmed by in vitro and in vivo experiments that P. micra can promote the development of CRC. Transcriptome analysis after the coincubation of bacteria and cells revealed that P. micra promoted cell proliferation by activating the Ras/ERK/c-Fos pathway. Furthermore, microRNA sequencing analysis of the cells and exosomes showed that miR-218-5p and protein tyrosine phosphatase receptor R (PTPRR) were the key factors involved in activating the Ras/ERK/c-Fos pathway, and the miR-218-5p inhibitor was used to confirm the role of microRNA in xenograft mice. Conclusion This experiment confirmed that P. micra promoted the development of CRC by upregulating miR-218-5p expression in cells and exosomes, inhibiting PTPRR expression, and ultimately activating the Ras/ERK/c-Fos signaling pathway.

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Section: Introductionmentioning

confidence: 99%