Increasing Italian ryegrass (<scp><i>Lolium multiflorum</i></scp> Lam.) regrowth via inoculation with an ammonia‐oxidizing bacterial strain

Wu, Di; Ma, Ke; Wang, Xiaoling; Lin, Qingfeng; Liu, Yuhua; Song, Peng; Liu, Wei; Zhang, Mingming; Zhao, Wei; Song, Chengwei

doi:10.1111/grs.12385

Cited by 3 publications

(1 citation statement)

References 56 publications

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“…Previous studies primarily focused on their vital role in nitrification processes within aquatic and soil environments ( Taylor et al, 2009 ; Kouki et al, 2011 ; Matsuno et al, 2013 ; Soliman and Eldyasti, 2018 ; Fang et al, 2019 ). A limited body of research has revealed that HAOB strains promote soil nitrification in the rhizosphere, leading to the release of nitrate nitrogen from the soil ( Wu et al, 2022 , 2023 ). However, our study introduces a novel perspective by exploring their role in enhancing plant growth.…”

Section: Discussionmentioning

confidence: 99%

Unveiling the dual role of heterotrophic ammonia-oxidizing bacteria: enhancing plant regrowth through modulating cytokinin delivery

Wang,

Si,

et al. 2023

Front. Microbiol.

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This study aims to investigate the dual impacts of heterotrophic ammonia-oxidizing bacteria (HAOB) strains on the regrowth of Italian ryegrass by studying cytokinin delivery from roots to leaves. The dual impacts encompass both the “soil-inside-role” and “soil-outside-role,” which refer to the HAOB operating inside and outside the rhizosphere soil within the rhizosphere microenvironment. The experimental design consisted of two sets of experiments, Exp-1 and Exp-2, involving different treatments. In Exp-1, various concentrations of NO3− were added to the roots to observe the soil-inside-role on cytokinin delivery from roots to leaves. In Exp-2, NO3− addition was combined with HAOB inoculation to observe the combined effects of the root-outside-role and root-inside-role on cytokinin synthesis and transport. The results indicated that NO3− concentrations ranging from 30 to 40 mmol L−1 had the most optimal effect on increasing leaf cytokinin content and delivery from roots to leaves, consequently promoting greater leaf regrowth biomass. When inoculated, the HAOB strain significantly increased rhizosphere soil nitrification rates under the soil-inside-role, leading to increased NO3− release from the soil and a subsequent boost in cytokinin delivery from roots to leaves. Additionally, the HAOB strain independently enhanced cytokinin delivery from roots to leaves outside the rhizosphere soil within the rhizosphere microenvironment, demonstrating its soil-outside-role. The combined effects of the soil-inside-role and soil-outside-role substantially increased leaf cytokinin content, playing a crucial role in promoting Italian ryegrass regrowth. The study’s findings shed light on the mechanisms through which HAOB can enhance plant growth by performing dual roles in the rhizosphere, offering potential applications in agriculture. Understanding the interaction between HAOB, cytokinins, and plant growth could lead to more effective strategies for improving crop productivity and promoting sustainable agriculture.

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Section: Discussionmentioning

confidence: 99%

Unveiling the dual role of heterotrophic ammonia-oxidizing bacteria: enhancing plant regrowth through modulating cytokinin delivery

Wang,

Si,

et al. 2023

Front. Microbiol.

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Effect of Ammonia-Oxidizing Bacterial Strains That Coexist in Rhizosphere Soil on Italian Ryegrass Regrowth

Wang

Zhu

et al. 2022

Microorganisms

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Potted Italian ryegrasses (Lolium multiflorum L.) were used to investigate the effect of ammonia-oxidizing bacterial (AOB) strain that coexisted in rhizosphere soil on Italian ryegrass regrowth. The results showed that the isolated and screened AOB strain (S2_8_1) had 100% similarity to Ensifer sesbaniae. The inoculation of S2_8_1 on day 44 before defoliation caused its copy number in rhizosphere soils to increase by 83–157% from day 34 before defoliation to day 14 after defoliation compared with that in Italian ryegrass without S2_8_1 inoculation, indicating that S2_8_1 coexisted permanently with Italian ryegrass. The coexistence promoted the delivery of root-derived cytokinin to leaves and to increase its cytokinin concentrations; thus, the Italian ryegrass regrowth accelerated. During the 14-day regrowth period, the S2_8_1 coexistence with Italian ryegrass caused its leaf and xylem sap cytokinin concentrations, rhizosphere soil nitrification rates, net photosynthetic rates, and total biomass to increase by 38%, 58%, 105%, 18%, and 39% on day 14 after defoliation, respectively. The inoculation of S2_8_1 on day 2 before defoliation also increased the regrowth of Italian ryegrass. Thus, the coexistence of AOB with Italian ryegrass increased its regrowth by regulating the delivery of cytokinins from roots to leaves.

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Dual Roles of Heterotrophic Ammonia-Oxidizing Bacteria in Enhancing Compensatory Growth upon Post-Drought in Maize

Yu,

Wang,

Sun

et al. 2024

Microorganisms

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This study investigates the mechanisms driving maize compensatory growth upon post-drought, to reveal how the root’s original cytokinins are regulated by the two-fold roles of heterotrophic bacteria with ammonia-oxidizing (HAOB) capabilities. The HAOB’ dual roles encompass influencing root cytokinin synthesis and transport through nitrification and a direct pathway. Experiment 1 involved introducing the application of varying amounts of NO3− to the roots to examine how nitrification affects cytokinin roots-to-leaves transport. Results demonstrate that the 30–40 mmol·L−1 NO3− concentration had ideal effects on enhancing post-drought growth in maize by facilitating cytokinin synthesis and transport. In experiment 2, an HAOB strain, S2_8_1, was utilized and NO3− was supplemented alongside HAOB inoculation to assess the joint impacts of nitrification and the direct pathway on the production and transportation of cytokinins. Results demonstrate that the HAOB strain S2_8_1 increases nitrification rates in rhizosphere soil, thereby promoting the transport of cytokinins from roots to leaves. In addition, the HAOB strain promotes root cytokinin transport to leaves autonomously, showcasing its direct pathway. Inoculation with the HAOB strain increased leaf cytokinin content and improved water use efficiency compared to the addition of NO3−; however, the combination of NO3− and HAOB strains resulted in a synergistic effect and further improvement. These findings elucidate how HAOB can enhance maize compensatory growth through its dual roles, presenting promising applications in agriculture.

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Increasing Italian ryegrass (Lolium multiflorum Lam.) regrowth via inoculation with an ammonia‐oxidizing bacterial strain

Cited by 3 publications

References 56 publications

Unveiling the dual role of heterotrophic ammonia-oxidizing bacteria: enhancing plant regrowth through modulating cytokinin delivery

Unveiling the dual role of heterotrophic ammonia-oxidizing bacteria: enhancing plant regrowth through modulating cytokinin delivery

Effect of Ammonia-Oxidizing Bacterial Strains That Coexist in Rhizosphere Soil on Italian Ryegrass Regrowth

Dual Roles of Heterotrophic Ammonia-Oxidizing Bacteria in Enhancing Compensatory Growth upon Post-Drought in Maize

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