Increasing paternal age and ejaculatory abstinence length negatively influence the intracytoplasmic sperm injection outcomes from egg‐sharing donation cycles

Abstract

Background:The paternal role in embryogenesis is much more than providing a haploid genome. The spermatozoa have crucial roles related to embryogenesis' epigenetic regulation, fusion of gametes, and cleavage, which may influence intracytoplasmic sperm injection outcomes. Objectives:To study whether paternal age, length of ejaculatory abstinence and semen parameters influence the outcomes of intracytoplasmic sperm injection in the oocyte recipients from egg-sharing donation cycles. Materials and methods:This historical cohort study involved 427 oocyte recipient intracytoplasmic sperm injection cycles, from 321 patients undergoing egg-sharing donation cycles from January 2015 to May 2017, in a private in vitro fertilization center.Electronic medical records of donors and recipients intracytoplasmic sperm injection cycles were reviewed. The impact of male age, length of ejaculatory abstinence, and semen parameters on recipients' intracytoplasmic sperm injection outcomes were investigated using general mixed models. Implantation, pregnancy, and live birth rates were the primary outcome measures. Results: Paternal age negatively affected fertilization, day 3 top-quality embryos, day 3 normal embryo cleavage speed, blastocyst formation, high-quality blastocysts, and implantation, and was correlated with diminished pregnancy and live birth rates. Length of ejaculatory abstinence negatively influenced the rates of day 3 top-quality embryos, normal embryo development on day 3, blastocyst development, and implantation. Sperm count positively affected the rates of fertilization, blastocyst development, and implantation. The percentage of progressive sperm motility positively affected day 3 normal embryo development rate and implantation rate. Total motile sperm count positively affected blastocyst development rate and implantation rate. Discussion and Conclusion: Additional tracking of the influence of male characteristics on intracytoplasmic sperm injection outcomes should be stimulated. Paternal age is not manageable, but reducing length of ejaculatory abstinence could be an alternative approach to improve the outcomes of intracytoplasmic sperm injection. | 595 SETTI ET al. How to cite this article: Setti AS, Braga DPAF, Iaconelli Junior A, Borges Junior E. Increasing paternal age and ejaculatory abstinence length negatively influence the intracytoplasmic sperm injection outcomes from egg-sharing donation cycles.

Two subsequent seminal productions: A good strategy to treat very severe oligoasthenoteratozoospermic infertile couples

“…Furthermore, an association has been observed between short abstinence and outcomes in various Assisted Reproduction Techniques (IUI, IVF, ICSI) 10,17 . Shorter abstinence time also seems to be related to increased pregnancy rates, embryo quality on day 3, blastocyst development and implantation following assisted reproduction 18–20 . Over many years of experience, we have observed that a second semen sample produced after a short time has different parameters, including a better motility.…”

“…10,17 Shorter abstinence time also seems to be related to increased pregnancy rates, embryo quality on day 3, blastocyst development and implantation following assisted reproduction. [18][19][20] Over many years of experience, we have observed that a second semen sample produced after a short time has different parameters, including a better motility. Supported by the literature, we started this study with the aim of verifying if it could be a good strategy to gain more motile spermatozoa for successful intracytoplasmic sperm injection (ICSI).…”

“…[15][16][17] Previous studies found that SARS, 1 of the 3 epidemic coronaviruses to emerge in the past 20 years and that shows similar clinical presentations to COVID-19, 18 could cause orchitis 19 and focal testicular atrophy. 20 Considering the tens of millions of COVID-19 cases and that men are more vulnerable to COVID-19 than women, [21][22][23] it is imperative to determine the effect of COVID-19 on male reproduction. 24 Several studies have been performed on this topic.…”

Impact of ejaculatory abstinence period and semen characteristic on the reproductive outcomes after intracytoplasmic sperm injection

“…Nevertheless, we found no significant association between sexual abstinence periods before ICSI and fertilization rate after the procedure. There are several studies with the same results in this regard (Colturato et al, 2007;Lee et al, 2015;Periyasamy et al, 2017;Borges Jr et al, 2019;Setti et al, 2020). Lee et al (2015) in a retrospective study have evaluated fertilization and pregnancy outcomes of 131 fresh ICSI cycles regarding different abstinence periods (2-4 days vs. 5-7 days).…”

“…However, several of the preceding studies have indicated the sexual abstinence period was associated with embryo development and pregnancy outcomes after the ICSI procedure (Colturato et al, 2007;Periyasamy et al, 2017;Borges Jr et al, 2019;Setti et al, 2020). Setti et al (2020) in a cohort study involving 427 oocyte recipient ICSI cycles showed that the abstinence period could affect embryo development and implantation. Moreover, in a prospective cohort study by Borges Jr et al (2019), data from 818 ICSI patients were analyzed and the results showed that the ejaculatory abstinence period could influence implantation and pregnancy rates.…”