Increasing platelet aggregability after venepuncture is platelet, not plasma derived

Terres, Wolfram; Becker, Bernhard F.; Kratzer, M. A. A.; Gerlach, E.

doi:10.1016/0049-3848(86)90217-3

Cited by 4 publications

(1 citation statement)

References 12 publications

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“…Since the antibody could abolish the effects of exogenous prostacyclin, the authors concluded that endogenous prostacyclin does not exist in significant quantities in the circulating blood to modify platelet function ex-vivo. Terres et al (32) did not observe diminished platelet aggregability when stored platelets were added to fresh plasma, nor was it affected when the donors had ingested acetyl salicylic acid. Based on these observations, the authors have concluded that increased platelet aggregability with the time after venepuncture may not be attributed to the disappearance of endogenous prostacyclin or formation of Haslam and Lynham (21) have reported activation of human platelet adenylate cyclase by Ad and 2ClAd at similar concentrations (without investigating their effects on platelet aggregation).…”

Section: Discussionmentioning

confidence: 92%

Effect of Dipyridamole on Spontaneous Platelet Aggregation in Whole Blood Decreases with the Time After Venepuncture: Evidence for the Role of ADP

et al. 1987

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SummarySpontaneous platelet aggregation (SPA) was studied in human whole blood at 3, 5, 10, 20, 30, 40 and 60 minutes after venepuncture. Using a whole blood platelet counter, SPA was quantified by measuring the fall in single platelet count upon rollermixing aliquots of citrated blood at 37° C. The extent of SPA increased with the time after venepuncture, with a correlation coefficient of 0.819. The inhibitory effect of dipyridamole (Dipy) on SPA was studied: (a) 10 μM at each time interval; (b) 0.5-100 μM at 3 and 30 minutes and (c) 15 μM in combination with 100 μM adenosine, 8 μM 2-chloroadenosine (2ClAd, an ADP receptor blocker) and 50 μM aspirin. There was a rapid decrease in the inhibitory effect of Dipy with the time after venepuncture; the correlation coefficient was -0.533. At all the concentrations studied, Dipy was more effective at 3 minutes than at 30 minutes after venepuncture. A combination of Dipy with adenosine, 2ClAd or aspirin was a more effective inhibitor of SPA than either drug alone. However, when 15 μM Dipy and 10 μM Ad were added together, the inhibitory effect of Dipy was not increased significantly, suggesting that Dipy inhibits platelet aggregation independent of Ad. The increase in SPA with the time after venepuncture was abolished when blood was taken directly into the anticoagulant containing 5 μM 2ClAd. It is suggested that ADP released from the red blood cells is responsible for the increased platelet aggregability with the time after venepuncture and makes a serious contribution to the artifacts of in vitro platelet function studies.

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Section: Discussionmentioning

confidence: 92%