Increasing Pregnancy by Improving Embryo Transfer Techniques

Madani, Tahereh; Jahangiri, Nadia

doi:10.5772/38165

Cited by 1 publication

(1 citation statement)

References 80 publications

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“…Embryo transfer was done according to Madani et al 2010 [16].Embryo / Blast cyst vitrification was done for good and fair embryos using the Irvine vitrification Vitrolife, Sweden) while thawing was done using Irvine warming medium (Irvine Scientific USA 90137-SO).…”

Section: Icsi Procedures and Embryo Scoringmentioning

confidence: 99%

Estimation of static Oxidation Reduction Potential in couples undergoing ICSI procedure.

et al. 2022

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There are some evidences suggest that an imbalance between levels of reactive oxygen species and antioxidants in semen plasma results in an oxidative state which leads to male infertility. Using this type of spermatozoa for Assisted Reproduction Techniques / Intra-Cytoplasmic Sperm Injection can result in losses before and after implantation, major / minor birth defects, and even childhood cancer. It is essential to develop techniques to detect cases with high free radical levels; a new promising analysis for assessing seminal oxidative stress is the static Oxidation-Reduction Potential estimation. Our study was conducted to identify clinical value of seminal oxidative stress estimation measured by MiOXSYS™ System and sperm DNA fragmentation in ICSI treatment strategy of male infertility. Semen analysis using World Health Organization (WHO) standard guidelines 2010 , Sperm DNA Fragmentation Indexby Sperm Chromatin Dispersion (SCD)test using Halosperm® Kit and static Oxidation-Reduction Potential by MiOXSYS™ System were assessed in potentially fertile (n = 25) and potentially infertile (n = 75) men. Potentially infertile patients had a significantly lower mean Sperm Count (47.06 10 6 sperm/ml vs. 96.88 10 6 sperm/ml), Normal Morphology (7.39% vs. 9.92%), Total Motility (42.17% vs. 53.92%), and A crosomal Index (45.6% vs. 57.6%). Conversely, potentially infertile patients had significantly higher ORP level (4.63 vs. 0.83) and non-significant (p = 0.388) higher Sperm DNA Fragmentation values than potentially fertile controls. Also potentially infertile patients had significantly higher Cumulative Pregnancy Result (62.67% vs. 36%) than potentially fertile controls. Oxidation Reduction Potential of the potentially infertile males showed a statistically significant negative correlation with each of Sperm Concentration (p<0.001), Normal Morphology (p<0.001), Total Motility (p=0.045), A crosomal Index (p=0.003) and statistically significant positive correlation with DNA Fragmentation Index (p=0.004). Oxidation Reduction Potential of the potentially fertile males showed statistically significant negative correlation with Sperm Count (p<0.001) only. Regarding ICSI outcome no correlation exists between seminal Oxidation Reduction Potential and Cumulative Pregnancy Results. The results of the study highlight the central value of oxidative stress estimation in male infertility, as it shows that assessing the semen antioxidant state and DNA integrity can be useful in the men visiting infertility centers for assessment of their fertility.

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Section: Icsi Procedures and Embryo Scoringmentioning

confidence: 99%