Increasing storage time of extended boar semen reduces sperm DNA integrity

Boe-Hansen, G.; Ersbøll, Annette Kjær; Greve, T.; Christensen, Preben

doi:10.1016/j.theriogenology.2004.09.006

Cited by 91 publications

(69 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similar results have been reported in previous research in boars (Boe-Hansen et al, 2005;De Ambrogi et al, 2006;Enciso et al, 2006) and in horses (López-Fernández et al, 2007).…”

Section: Discussionsupporting

confidence: 92%

Seasonal variation in sperm characteristics of boars in southern Uruguay

2015

View full text Add to dashboard Cite

-The objective of this study was to evaluate the effects of season, natural photoperiod, and room temperature at the housing facility on boar semen characteristics in Uruguay (34º66'S; 56º29'W). For this purpose, 117 ejaculates, obtained from eight adult males collected through 12 consecutive months, were assessed for sperm viability, DNA integrity, abnormalities (total, primary, and secondary), ejaculate volume, and sperm concentration. Viability, total and primary abnormalities, volume, and sperm concentration were affected by season. Sperm viability, volume, and sperm concentration were affected by natural photoperiod. In general, autumn and the decreasing photoperiod had a negative impact on most of the semen characteristics, except for volume. Housing temperature did not affect semen characteristics. In boars living in temperate climates, semen quality is negatively affected during autumn and is related to photoperiod changes; however, the effects of temperature changes in housingdo not affect these seminal characteristics. In this scenario, seasonal differences in semen quality may have a negative effect on sow fertilization. Consequently, semen quality control especially during autumn is imperative for the best boar selection to be used for insemination purposes. Seasonal differences in semen quality may have a negative effect on sow reproductive performance. This issue will be addressed in a future investigation.

show abstract

“…Similar results have been reported in previous research in boars (Boe-Hansen et al, 2005;De Ambrogi et al, 2006;Enciso et al, 2006) and in horses (López-Fernández et al, 2007).…”

Section: Discussionsupporting

confidence: 92%

Seasonal variation in sperm characteristics of boars in southern Uruguay

2015

View full text Add to dashboard Cite

show abstract

“…In our study, a significant reduction of the integrity of chromatin was found on the 2 nd day compared to the 1 st day of the experiment for all diluents. This finding reinforces the results of Boe-Hansen et al (2005) who found that after 72 h of sperm storage the integrity of sperm DNA chromatin was significantly reduced. In contrast, De Ambrogi et al (2006) did not reveal any difference in the integrity of the sperm chromatin associated with different diluents or preservation time after sperm storage for 96 h at 17 °C.…”

Section: P-valuesupporting

confidence: 92%

A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

et al. 2016

View full text Add to dashboard Cite

The present study compared the quality characteristics of boar semen diluted with three extenders of different proposed preservation times (short-term, medium-term and long-term). A part of extended semen was used for artificial insemination on the farm (30 sows/extender), while the remaining part was stored for three days (16-18 °C). Stored and used semen was also laboratory assessed at insemination time, on days 1 and 2 after the collection (day 0). The long-term extender was used for a short time, within 2 days from semen collection, with the aim to investigate a possible advantage over the others regarding laboratory or farm fertility indicators at the beginning of the preservation time. Viability, motility, kinetic indicators, morphology and DNA fragmentation were estimated. The results showed reduced viability, higher values for most of the kinetics, and higher immotile spermatozoa from day 1 to day 2 in all extenders; however, the long-term extender was superior compared to the other two on both days. With regard to morphology and chromatin integrity, the percentage of abnormal and fragmented spermatozoa increased on day 2 compared to day 1 for all of the extenders. However, based on the farrowing rate and the number of piglets born alive after the application of conventional artificial insemination within 2 days from semen collection/dilution, it was found that the medium-term diluents were more effective. In conclusion, it seems that the in vivo fertilization process involves more factors than simply the quality of laboratory evaluated sperm indicators, warranting further research. DNA fragmentation, ejaculate, in vitro quality, in vivo fertility, semen analysis, semen storageConventional examination of boar sperm on commercial farms aims to evaluate the fertilizing capacity and to control the conditions of breeding and sperm manipulation after the collection of the ejaculate (Tsakmakidis et al. 2010). Semen quality characteristics are affected by many factors, including the type of semen extender, storage duration and temperature (Fraser and Strzezek 2004).Boar semen diluents are classified by the commercial providers into three categories: short-term (ST), medium-term (MT) and long term (LT) extenders according to their preservation capacity (1-2, 3-4 and 7-10 days after collection, respectively). Long-term extenders allow for long distance sperm transportation. Moreover, the acquired time flexibility enables collection centres to conduct specialized diagnostic tests and to better organize semen procedures (Gadea 2003).In literature, only few studies compare the preservation capacity of different extenders in the laboratory and the relation with actual field conditions. To our knowledge, data on the efficacy of swine artificial insemination (AI) 1-2 days from collection and dilution in long-term extenders are even scarcer. This category of extenders is supposed to maintain sperm quality within acceptable levels (i.e. total sperm number > 35 × 10 9 sperm/ejaculate, gross motility > 70%, abnorma...

show abstract

“…The cause of idiopathic infertility may be a high index of DNA fragmentation in chromatin [57][58][59][60][61]. The DNA fragmentation in spermatozoa also depends on numerous biotic and abiotic factors [62][63][64][65][66][67][68][69].…”

mentioning

confidence: 99%

Biological Integrity of Bison Epididymal Sperm Under Cryoconservation and Long Storage

Иолчиев¹,

Абилов²,

Таджиева³

et al. 2017

S-h. biol.

View full text Add to dashboard Cite

A b s t r a c tConservation of biodiversity is one of the global challenges of the modern world. The preservation of animal genetic resources is considered essential for the food supply, since sustainable food production appears to be the greatest problem due to the human population growth, depletion of the Earth's natural resources, and many species becoming endangered. In situ and ex situ methods of preservation of the species (i.e. in/out of their natural habitats, respectively) are two major approaches to animal biodiversity conservation. Ex situ strategy involves the techniques for the genetic material cryopreservation. Cryopreservation of the wildlife biomaterials allows to use these genetic resources not only for the conservation and the renewal, but also for the introduction into the genotype of the farm animals. The bison (Bison bonasus) is identified as the rare and endangered species. At present, the free-living bison population in Russia comprises more than 1500 animals. A research concept of the Russian bison gene pool preservation includes creating cryo-preserved pool of bison spermatozoa. In this paper we report findings on biological adequacy of the cryopreserved epididymal bison semen after the long storage (for more than 20 years). The sperm samples were collected postmortem from the testicular appendages of four bison males sustained the injuries incompatible with life or culled and used for hunting. For the assessment of semen motility we used a computerassisted semen analysis (CASA) device; the DNA fragmentation index was assessed in AO-test with the acridine orange staining. The acrosomal integrity was studied by Diff-Quik staining method. It was shown that the semen quality parameters differed significantly due to the individual peculiarities of the bison. The spermatozoa of A + B grade which performed good motility and rectilinear motion reached more than 28 % in the semen of the males Mutfil and Morus, while in the Avel's and Misir's semen over 67 % spermatozoa were non-motile and 12.1 % and 10.4 % spermatozoa exhibited rotational and vibrational motions, respectively. The frequency of spermatozoa with pathomorphological changes significantly varied depending on the individual properties of the bison, with the greatest and the lowest values of 14.6 % and 6.8 %, respectively. The DNA fragmentation index reflecting sperm chromatin integrity can depend on the numerous biotic and abiotic factors and may vary in great ranges. In our surveys, it varied from 7 % to 86 %. For all the morphometric parameters, except the head width, the bison spermatozoa were inferior to the spermatozoa of the bulls though the differences between animal groups were not statistically significant. However, the area of the spermatozoa head in bulls was 3.14 m 2 larger than that of bison.

show abstract

Increasing storage time of extended boar semen reduces sperm DNA integrity

Cited by 91 publications

References 28 publications

Seasonal variation in sperm characteristics of boars in southern Uruguay

Seasonal variation in sperm characteristics of boars in southern Uruguay

A comparative study of boar semen extenders with different proposed preservation times and their effect on semen quality and fertility

Biological Integrity of Bison Epididymal Sperm Under Cryoconservation and Long Storage

Contact Info

Product

Resources

About