2011
DOI: 10.1002/anie.201101817
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Increasing the Efficacy of Bioorthogonal Click Reactions for Bioconjugation: A Comparative Study

Abstract: Raising the bar: The efficacy of bioorthogonal reactions for bioconjugation has been thoroughly evaluated in four different biological settings. Powered by the development of new biocompatible ligands, the copper‐catalyzed azide–alkyne cycloaddition (see picture) has brought about unsurpassed bioconjugation efficiency, and thus it holds great promise as a highly potent and adaptive tool for a broader spectrum of biological applications.

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Cited by 397 publications
(321 citation statements)
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“…21 We have recently shown that the isonitrile group can also be used as a novel chemical reporter group for glycan metabolic labelling. 22 Azides incorporated in cell surface glycoproteins can be detected using either the Staudinger ligation, 23 the copper catalysed [3 + 2] cycloaddition with alkynes (with biologically compatible catalysts) 24 or the copper-free [3 + 2] cycloaddition with strained cyclooctynes. 25 Cyclopropenes and alkenes, on the other hand, can react with tetrazines.…”
Section: Introductionmentioning
confidence: 99%
“…21 We have recently shown that the isonitrile group can also be used as a novel chemical reporter group for glycan metabolic labelling. 22 Azides incorporated in cell surface glycoproteins can be detected using either the Staudinger ligation, 23 the copper catalysed [3 + 2] cycloaddition with alkynes (with biologically compatible catalysts) 24 or the copper-free [3 + 2] cycloaddition with strained cyclooctynes. 25 Cyclopropenes and alkenes, on the other hand, can react with tetrazines.…”
Section: Introductionmentioning
confidence: 99%
“…tris(triazolylmethyl)amines) were developed. 146,[151][152][153] When these ligand accelerated CuAAC reactions were used in vitro, live cells showed normal functions after the treatment evidencing their applicability in living systems. 146,[151][152][153] The reaction time of CuAAC reaction with these accelerator ligands was optimized for 5-10 min, because in longer reaction time significant cell death was observed.…”
mentioning
confidence: 93%
“…146,[151][152][153] When these ligand accelerated CuAAC reactions were used in vitro, live cells showed normal functions after the treatment evidencing their applicability in living systems. 146,[151][152][153] The reaction time of CuAAC reaction with these accelerator ligands was optimized for 5-10 min, because in longer reaction time significant cell death was observed. 146,147,151,154 In Kennedy's work L-histidine (His) was used as accelerator ligand that resulted in a two-to threefold slower reaction as compared to other ligands (e.g.…”
mentioning
confidence: 93%
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