Concern for the safety and public health of the environmental impact of the use of chemical pesticides has prompted more consideration of the use of environmentally friendly plant cultivation technologies as a natural approach to improve plant health. This study aimed to evaluate the effectiveness of seed treatment with rhizobacteria Bacillus sp.CKD061, Pseudomonas sp. SWRIIB04 and Pseudomonas fluorescens PG01 alone or in mixture on the growth and yield of hot pepper plants. The study was arranged using a randomized block design consisting of 8 rhizobacteria treatments, namely control, Bacillus sp.CKD061 (C), Pseudomonas sp. SWRIIB04 (S), P. fluorescens PG01 (P), mixture of Bacillus sp.CKD061 and Pseudomonas sp. SWRIIB04 (C+S), mixture of Bacillus sp.CKD061 and P. fluorescens PG01 (C+P), mixture of Pseudomonas sp. SWRIIB04 and P. fluorescens PG01 (S+P), mixture of Bacillus sp.CKD061, Pseudomonas sp. SWRIIB04 and P. fluorescens PG01 (C+S+P). The data obtained were analyzed using analysis of variance, followed by Duncan’s Multiple Range Test. The results showed that the seed treatment using a mixture of rhizobacteria Bacillus sp.CKD061 and Pseudomonas sp. SWRIIB04 was the most effective treatment in increasing the growth and yield of hot pepper plants. The increase in hot pepper production reached 90% compared to the control.