Increasing the Productivity of Peroxidase‐Catalyzed Oxyfunctionalization: A Case Study on the Potential of Two‐Liquid‐Phase Systems

Abstract

Biocatalysis, which occurs mostly in aqueous reaction media, is generally incompatible with the hydrophobic nature of many reactants. This may be overcome by using the two‐liquid‐phase system (2 LPS) approach. We demonstrate the beneficial application of the 2 LPS approach for the photobiocatalytic oxyfunctionalization of thioanisole. Compared to aqueous reaction conditions, the 2 LPS approach not only allowed for higher substrate loadings (without the use of additional solvents) but also increased the productivity and robustness of the overall reaction.

Selective Photooxidation Reactions using Water‐Soluble Anthraquinone Photocatalysts

“…However, at present we also cannot fully rule out that the diffusion of O 2 into the reaction system may become overall rate limiting at high overall conversion rates. Previously, we observed similar effects in the case of photoenzymatic oxidation reactions …”

Photoenzymatic Hydroxylation of Ethylbenzene Catalyzed by Unspecific Peroxygenase: Origin of Enzyme Inactivation and the Impact of Light Intensity and Temperature

“…Many strategies are known for a H 2 O 2 supply in adequate concentrations, especially in situ generation by reduction of molecular oxygen is often applied e. g. via chemical, enzymatic, electrochemical or photochemical approaches to alleviate the instability issue, see Table S4 for a detailed comparison of the methods on the example of Aae UPO catalyzed hydroxylation of ethylbenzene.…”

Towards preparative peroxygenase-catalyzed oxyfunctionalization reactions in organic media

“…In a previous study using a comparable setup a volumetric productivity around 2.5 mM h -1 had been observed, which was attributed to diffusion limitation over the interphase. [20] Also the robustness of this preliminary setup was comparably poor with a complete loss of ethylbenzene hydroxylation activity after 24h.…”