Increasing the Protein Amount of Chlorella vulgaris [Chlorphyta] Strains Isolated from Different Fresh Water Ponds

Abstract

Chlorella vulgaris is a single-cell, spherical green algae and one of the microalgae on which many applied studies are conducted. In the present study, five strains displaying fast and efficient reproduction were chosen among 11 C. vulgaris strains isolated from different fresh water ponds and their cell numbers and the amount of chlorophyll a, protein, lipid, cellulose and carbohydrate were examined. The main goal of the study is to investigate increasing the biochemical contents especially the protein content of C. vulgaris strains in different mediums. In the present study, cell densities were determined through cell count for five days. In parallel with cell count, their chlorophyll a content was determined. The highest cell density was observed with C. vulgaris TOH (Tourism and Hotel Management Pond) strain as 5.5 × 10 4 h/mL, and the chlorophyll a content as 4.3 × 10 2 mg/m 3 . The highest intracellular protein amount was determined with C. vulgaris GUH (Gazi University Rectorship Pond) (0.061 g/100 mL) and the highest lipid amount was attained with C. vulgaris UIK (Ulus Construction Well) strain as 0.019 g/100 mL. The process of increasing the intracellular protein amount in C. vulgaris GUH strain was carried out in Prat, Yagojinski and Chlorella medium. The results indicated that Chlorella medium increased the intracellular protein amount.